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B8JF03 (RUVC_ANAD2) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 34. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Crossover junction endodeoxyribonuclease RuvC

EC=3.1.22.4
Alternative name(s):
Holliday junction nuclease RuvC
Holliday junction resolvase RuvC
Gene names
Name:ruvC
Ordered Locus Names:A2cp1_1009
OrganismAnaeromyxobacter dehalogenans (strain 2CP-1 / ATCC BAA-258) [Complete proteome] [HAMAP]
Taxonomic identifier455488 [NCBI]
Taxonomic lineageBacteriaProteobacteriaDeltaproteobacteriaMyxococcalesCystobacterineaeMyxococcaceaeAnaeromyxobacter

Protein attributes

Sequence length185 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Nuclease that resolves Holliday junction intermediates in genetic recombination. Cleaves the cruciform structure in supercoiled DNA by nicking to strands with the same polarity at sites symmetrically opposed at the junction in the homologous arms and leaves a 5'-terminal phosphate and a 3'-terminal hydroxyl group By similarity. HAMAP-Rule MF_00034

Catalytic activity

Endonucleolytic cleavage at a junction such as a reciprocal single-stranded crossover between two homologous DNA duplexes (Holliday junction). HAMAP-Rule MF_00034

Cofactor

Binds 1 magnesium ion per subunit By similarity. HAMAP-Rule MF_00034

Sequence similarities

Belongs to the RuvC family.

Ontologies

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 185185Crossover junction endodeoxyribonuclease RuvC HAMAP-Rule MF_00034
PRO_1000195232

Sites

Metal binding71Magnesium By similarity
Metal binding661Magnesium By similarity
Metal binding1371Magnesium By similarity
Metal binding1401Magnesium By similarity

Sequences

Sequence LengthMass (Da)Tools
B8JF03 [UniParc].

Last modified March 3, 2009. Version 1.
Checksum: A51F32EF41AA9CB0

FASTA18518,924
        10         20         30         40         50         60 
MIVLGIDPGS RRCGYGVVAR EGARLTVVES GVLVPGDLPM AQRLGRILDG LDALIARARP 

        70         80         90        100        110        120 
LEASVESVFS GASPRSALVL GQARGVALAA AARAGLPVFE YAPSEVKLAF TGNGRAGKDQ 

       130        140        150        160        170        180 
MLRTARMLLG AAPGLSDEAD ALAIAVCHLA RRAFAVPAAG AGRAAAARAA AARLRPSRRD 


HRGTP 

« Hide

References

[1]"Complete sequence of Anaeromyxobacter dehalogenans 2CP-1."
Lucas S., Copeland A., Lapidus A., Glavina del Rio T., Dalin E., Tice H., Bruce D., Goodwin L., Pitluck S., Saunders E., Brettin T., Detter J.C., Han C., Larimer F., Land M., Hauser L., Kyrpides N., Ovchinnikova G., Beliaev A.S., Richardson P.
Submitted (JAN-2009) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: 2CP-1 / ATCC BAA-258.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP001359 Genomic DNA. Translation: ACL64360.1.
RefSeqYP_002491426.1. NC_011891.1.

3D structure databases

ProteinModelPortalB8JF03.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING455488.A2cp1_1009.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaACL64360; ACL64360; A2cp1_1009.
GeneID7297055.
KEGGacp:A2cp1_1009.
PATRIC20908882. VBIAnaDeh28364_1042.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0817.
HOGENOMHOG000012181.
KOK01159.
OMAARGACIT.
OrthoDBEOG6RG044.

Enzyme and pathway databases

BioCycADEH455488:GH35-1015-MONOMER.

Family and domain databases

Gene3D3.30.420.10. 1 hit.
HAMAPMF_00034. RuvC.
InterProIPR012337. RNaseH-like_dom.
IPR002176. X-over_junc_endoDNase_RuvC.
[Graphical view]
PfamPF02075. RuvC. 1 hit.
[Graphical view]
PRINTSPR00696. RSOLVASERUVC.
SUPFAMSSF53098. SSF53098. 1 hit.
TIGRFAMsTIGR00228. ruvC. 1 hit.
ProtoNetSearch...

Entry information

Entry nameRUVC_ANAD2
AccessionPrimary (citable) accession number: B8JF03
Entry history
Integrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: March 3, 2009
Last modified: May 14, 2014
This is version 34 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families