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Protein

Lipid-A-disaccharide synthase

Gene

lpxB

Organism
Anaeromyxobacter dehalogenans (strain 2CP-1 / ATCC BAA-258)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Condensation of UDP-2,3-diacylglucosamine and 2,3-diacylglucosamine-1-phosphate to form lipid A disaccharide, a precursor of lipid A, a phosphorylated glycolipid that anchors the lipopolysaccharide to the outer membrane of the cell.UniRule annotation

Catalytic activityi

UDP-2-N,3-O-bis((3R)-3-hydroxytetradecanoyl)-alpha-D-glucosamine + 2-N,3-O-bis((3R)-3-hydroxytetradecanoyl)-alpha-D-glucosaminyl 1-phosphate = UDP + 2-N,3-O-bis((3R)-3-hydroxytetradecanoyl)-beta-D-glucosaminyl-(1->6)-2-N,3-O-bis((3R)-3-hydroxytetradecanoyl)-alpha-D-glucosaminyl 1-phosphate.UniRule annotation

Pathway:ilipid IV(A) biosynthesis

This protein is involved in step 5 of the subpathway that synthesizes lipid IV(A) from (3R)-3-hydroxytetradecanoyl-[acyl-carrier-protein] and UDP-N-acetyl-alpha-D-glucosamine.UniRule annotation
Proteins known to be involved in the 6 steps of the subpathway in this organism are:
  1. Acyl-[acyl-carrier-protein]--UDP-N-acetylglucosamine O-acyltransferase (lpxA)
  2. UDP-3-O-[3-hydroxymyristoyl] N-acetylglucosamine deacetylase (lpxC), UDP-3-O-[3-hydroxymyristoyl] N-acetylglucosamine deacetylase (lpxC)
  3. no protein annotated in this organism
  4. no protein annotated in this organism
  5. Lipid-A-disaccharide synthase (lpxB)
  6. Tetraacyldisaccharide 4'-kinase (lpxK)
This subpathway is part of the pathway lipid IV(A) biosynthesis, which is itself part of Glycolipid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes lipid IV(A) from (3R)-3-hydroxytetradecanoyl-[acyl-carrier-protein] and UDP-N-acetyl-alpha-D-glucosamine, the pathway lipid IV(A) biosynthesis and in Glycolipid biosynthesis.

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Keywords - Biological processi

Lipid A biosynthesis, Lipid biosynthesis, Lipid metabolism

Enzyme and pathway databases

BioCyciADEH455488:GH35-2843-MONOMER.
UniPathwayiUPA00359; UER00481.

Names & Taxonomyi

Protein namesi
Recommended name:
Lipid-A-disaccharide synthaseUniRule annotation (EC:2.4.1.182UniRule annotation)
Gene namesi
Name:lpxBUniRule annotation
Ordered Locus Names:A2cp1_2806
OrganismiAnaeromyxobacter dehalogenans (strain 2CP-1 / ATCC BAA-258)
Taxonomic identifieri455488 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaDeltaproteobacteriaMyxococcalesCystobacterineaeAnaeromyxobacteraceaeAnaeromyxobacter
ProteomesiUP000007089 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 383383Lipid-A-disaccharide synthasePRO_1000191460Add
BLAST

Structurei

3D structure databases

ProteinModelPortaliB8JE78.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the LpxB family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0763.
HOGENOMiHOG000018004.
KOiK00748.
OMAiTVHFVCP.
OrthoDBiEOG6FBWZR.

Family and domain databases

HAMAPiMF_00392. LpxB.
InterProiIPR003835. Glyco_trans_19.
[Graphical view]
PfamiPF02684. LpxB. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00215. lpxB. 1 hit.

Sequencei

Sequence statusi: Complete.

B8JE78-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLYSPRLTDQ ILIVAGEASA DLHAARTLHE LQRLRPGITA FGVGGPRLRE
60 70 80 90 100
AGLEALAPAE DISVMGLAEV LPRIPRILGI LRMLGRAAAE RRPRAALLVD
110 120 130 140 150
LPDFNLRLAA RLKKLGIPVV YYVSPTIWAW RQGRAKKIAR VVDRMLCILP
160 170 180 190 200
FEERFYEGTG VSARFVGHPF AERPPPGPAE AYRSALGLPA SRTTIAMVPG
210 220 230 240 250
SRPSELKRLL PPMLQAAERL RAAHPDAQFV VPVAPTLDRA ALEPYLAAHR
260 270 280 290 300
TLEVRLVDGR TEEVVGASDA ALVKSGTSTL EAGLMLRPMV VVYKLSWLSY
310 320 330 340 350
AVARMLVKIA HVALVNILAG RGIVPELLQG DASPERMAAE VERLLGDRAA
360 370 380
REAQIAALRE VRASLGEPGA PLRVAEEVLG VMR
Length:383
Mass (Da):41,563
Last modified:March 3, 2009 - v1
Checksum:i15B37DFA38E85FBB
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001359 Genomic DNA. Translation: ACL66143.1.
RefSeqiWP_012633900.1. NC_011891.1.

Genome annotation databases

EnsemblBacteriaiACL66143; ACL66143; A2cp1_2806.
KEGGiacp:A2cp1_2806.
PATRICi20912541. VBIAnaDeh28364_2845.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001359 Genomic DNA. Translation: ACL66143.1.
RefSeqiWP_012633900.1. NC_011891.1.

3D structure databases

ProteinModelPortaliB8JE78.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiACL66143; ACL66143; A2cp1_2806.
KEGGiacp:A2cp1_2806.
PATRICi20912541. VBIAnaDeh28364_2845.

Phylogenomic databases

eggNOGiCOG0763.
HOGENOMiHOG000018004.
KOiK00748.
OMAiTVHFVCP.
OrthoDBiEOG6FBWZR.

Enzyme and pathway databases

UniPathwayiUPA00359; UER00481.
BioCyciADEH455488:GH35-2843-MONOMER.

Family and domain databases

HAMAPiMF_00392. LpxB.
InterProiIPR003835. Glyco_trans_19.
[Graphical view]
PfamiPF02684. LpxB. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00215. lpxB. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. "Complete sequence of Anaeromyxobacter dehalogenans 2CP-1."
    Lucas S., Copeland A., Lapidus A., Glavina del Rio T., Dalin E., Tice H., Bruce D., Goodwin L., Pitluck S., Saunders E., Brettin T., Detter J.C., Han C., Larimer F., Land M., Hauser L., Kyrpides N., Ovchinnikova G., Beliaev A.S., Richardson P.
    Submitted (JAN-2009) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 2CP-1 / ATCC BAA-258.

Entry informationi

Entry nameiLPXB_ANAD2
AccessioniPrimary (citable) accession number: B8JE78
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: March 3, 2009
Last modified: July 22, 2015
This is version 40 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.