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B8J9N1

- GLMU_ANAD2

UniProt

B8J9N1 - GLMU_ANAD2

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Protein

Bifunctional protein GlmU

Gene

glmU

Organism
Anaeromyxobacter dehalogenans (strain 2CP-1 / ATCC BAA-258)
Status
Reviewed - Annotation score: 4 out of 5- Protein inferred from homologyi

Functioni

Catalyzes the last two sequential reactions in the de novo biosynthetic pathway for UDP-N-acetylglucosamine (UDP-GlcNAc). The C-terminal domain catalyzes the transfer of acetyl group from acetyl coenzyme A to glucosamine-1-phosphate (GlcN-1-P) to produce N-acetylglucosamine-1-phosphate (GlcNAc-1-P), which is converted into UDP-GlcNAc by the transfer of uridine 5-monophosphate (from uridine 5-triphosphate), a reaction catalyzed by the N-terminal domain.UniRule annotation

Catalytic activityi

Acetyl-CoA + alpha-D-glucosamine 1-phosphate = CoA + N-acetyl-alpha-D-glucosamine 1-phosphate.UniRule annotation
UTP + N-acetyl-alpha-D-glucosamine 1-phosphate = diphosphate + UDP-N-acetyl-alpha-D-glucosamine.UniRule annotation

Cofactori

Binds 1 magnesium ion per subunit.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei27 – 271UDP-GlcNAcUniRule annotation
Binding sitei82 – 821UDP-GlcNAcUniRule annotation
Metal bindingi112 – 1121MagnesiumUniRule annotation
Binding sitei149 – 1491UDP-GlcNAc; via amide nitrogenUniRule annotation
Binding sitei164 – 1641UDP-GlcNAcUniRule annotation
Binding sitei179 – 1791UDP-GlcNAcUniRule annotation
Metal bindingi235 – 2351MagnesiumUniRule annotation
Binding sitei235 – 2351UDP-GlcNAcUniRule annotation
Binding sitei341 – 3411Acetyl-CoA; amide nitrogenUniRule annotation
Binding sitei359 – 3591Acetyl-CoAUniRule annotation
Active sitei371 – 3711Proton acceptorUniRule annotation
Binding sitei374 – 3741Acetyl-CoAUniRule annotation
Binding sitei385 – 3851Acetyl-CoAUniRule annotation
Binding sitei413 – 4131Acetyl-CoAUniRule annotation
Binding sitei431 – 4311Acetyl-CoA; via amide nitrogenUniRule annotation
Binding sitei448 – 4481Acetyl-CoAUniRule annotation

GO - Molecular functioni

  1. glucosamine-1-phosphate N-acetyltransferase activity Source: UniProtKB-HAMAP
  2. magnesium ion binding Source: UniProtKB-HAMAP
  3. UDP-N-acetylglucosamine diphosphorylase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. cell morphogenesis Source: UniProtKB-HAMAP
  2. cell wall organization Source: UniProtKB-KW
  3. lipid A biosynthetic process Source: UniProtKB-UniPathway
  4. lipopolysaccharide biosynthetic process Source: InterPro
  5. peptidoglycan biosynthetic process Source: UniProtKB-HAMAP
  6. regulation of cell shape Source: UniProtKB-KW
  7. UDP-N-acetylglucosamine biosynthetic process Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Acyltransferase, Nucleotidyltransferase, Transferase

Keywords - Biological processi

Cell shape, Cell wall biogenesis/degradation, Peptidoglycan synthesis

Keywords - Ligandi

Magnesium, Metal-binding

Enzyme and pathway databases

BioCyciADEH455488:GH35-4157-MONOMER.
UniPathwayiUPA00113; UER00532.
UPA00113; UER00533.
UPA00973.

Names & Taxonomyi

Protein namesi
Recommended name:
Bifunctional protein GlmUUniRule annotation
Including the following 2 domains:
UDP-N-acetylglucosamine pyrophosphorylaseUniRule annotation (EC:2.7.7.23UniRule annotation)
Alternative name(s):
N-acetylglucosamine-1-phosphate uridyltransferaseUniRule annotation
Glucosamine-1-phosphate N-acetyltransferaseUniRule annotation (EC:2.3.1.157UniRule annotation)
Gene namesi
Name:glmUUniRule annotation
Ordered Locus Names:A2cp1_4101
OrganismiAnaeromyxobacter dehalogenans (strain 2CP-1 / ATCC BAA-258)
Taxonomic identifieri455488 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaDeltaproteobacteriaMyxococcalesCystobacterineaeAnaeromyxobacteraceaeAnaeromyxobacter
ProteomesiUP000007089: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 488488Bifunctional protein GlmUPRO_1000186392Add
BLAST

Interactioni

Subunit structurei

Homotrimer.UniRule annotation

Protein-protein interaction databases

STRINGi455488.A2cp1_4101.

Structurei

3D structure databases

ProteinModelPortaliB8J9N1.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 237237PyrophosphorylaseUniRule annotationAdd
BLAST
Regioni13 – 164UDP-GlcNAc bindingUniRule annotation
Regioni87 – 882UDP-GlcNAc bindingUniRule annotation
Regioni110 – 1123UDP-GlcNAc bindingUniRule annotation
Regioni238 – 25821LinkerUniRule annotationAdd
BLAST
Regioni259 – 488230N-acetyltransferaseUniRule annotationAdd
BLAST
Regioni394 – 3952Acetyl-CoA bindingUniRule annotation

Sequence similaritiesi

In the N-terminal section; belongs to the N-acetylglucosamine-1-phosphate uridyltransferase family.UniRule annotation
In the C-terminal section; belongs to the transferase hexapeptide repeat family.UniRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiCOG1207.
HOGENOMiHOG000283476.
KOiK04042.
OMAiIGERAYI.
OrthoDBiEOG6Z6FQZ.

Family and domain databases

Gene3Di3.90.550.10. 1 hit.
HAMAPiMF_01631. GlmU.
InterProiIPR005882. Bifunctional_GlmU.
IPR001451. Hexapep_transf.
IPR018357. Hexapep_transf_CS.
IPR025877. MobA-like_NTP_Trfase_dom.
IPR029044. Nucleotide-diphossugar_trans.
IPR011004. Trimer_LpxA-like.
[Graphical view]
PfamiPF00132. Hexapep. 3 hits.
PF12804. NTP_transf_3. 1 hit.
[Graphical view]
SUPFAMiSSF51161. SSF51161. 1 hit.
SSF53448. SSF53448. 1 hit.
TIGRFAMsiTIGR01173. glmU. 1 hit.
PROSITEiPS00101. HEXAPEP_TRANSFERASES. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

B8J9N1-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MPRTRTPLAA IVLAAGKGTR MKSNKAKVLH EVAGRPLAYY PVKRALELGA
60 70 80 90 100
SPVVVVVGHQ AEAVEAALSA ALPEAPLRFA VQEQQLGTAH AVLAAKRALR
110 120 130 140 150
GYRGPVLILS GDTPLLRAET LEAVVSAGRR ARAAVSLATM TLEVPRGYGR
160 170 180 190 200
VVRDARGRPA RIVEEKDATE AERAVREVNA GLYCVDAELL WKKLAKVGTA
210 220 230 240 250
NAQREFYLTD LVPMAAQAGG VAGVEVPAEE ASGVNDRVEL SRANRVMVGR
260 270 280 290 300
LAEAFMRAGV TIEDPARFDC DEGVEIGADA VIEPNVRLRG RTRVGARTRV
310 320 330 340 350
GVGAVITDGV LADGVTVNPY TVISEAQVAE GAILGPFSRL RPGADIGPEA
360 370 380 390 400
HVGNFVEVKK SRLGKGAKAN HLAYLGDAEI GAGANIGAGT ITCNYDGERK
410 420 430 440 450
NPTRIGEGAF IGSDSILVAP IEIGAGAYVA AGSTLTDPVP AGALALGRAR
460 470 480
QVTKEGWVAQ RQAEKQMKGT ATGPASARKG RPAARRAS
Length:488
Mass (Da):50,902
Last modified:March 3, 2009 - v1
Checksum:i889645503870632F
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP001359 Genomic DNA. Translation: ACL67419.1.
RefSeqiYP_002494485.1. NC_011891.1.

Genome annotation databases

EnsemblBacteriaiACL67419; ACL67419; A2cp1_4101.
GeneIDi7297526.
KEGGiacp:A2cp1_4101.
PATRICi20915156. VBIAnaDeh28364_4136.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP001359 Genomic DNA. Translation: ACL67419.1 .
RefSeqi YP_002494485.1. NC_011891.1.

3D structure databases

ProteinModelPortali B8J9N1.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 455488.A2cp1_4101.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ACL67419 ; ACL67419 ; A2cp1_4101 .
GeneIDi 7297526.
KEGGi acp:A2cp1_4101.
PATRICi 20915156. VBIAnaDeh28364_4136.

Phylogenomic databases

eggNOGi COG1207.
HOGENOMi HOG000283476.
KOi K04042.
OMAi IGERAYI.
OrthoDBi EOG6Z6FQZ.

Enzyme and pathway databases

UniPathwayi UPA00113 ; UER00532 .
UPA00113 ; UER00533 .
UPA00973 .
BioCyci ADEH455488:GH35-4157-MONOMER.

Family and domain databases

Gene3Di 3.90.550.10. 1 hit.
HAMAPi MF_01631. GlmU.
InterProi IPR005882. Bifunctional_GlmU.
IPR001451. Hexapep_transf.
IPR018357. Hexapep_transf_CS.
IPR025877. MobA-like_NTP_Trfase_dom.
IPR029044. Nucleotide-diphossugar_trans.
IPR011004. Trimer_LpxA-like.
[Graphical view ]
Pfami PF00132. Hexapep. 3 hits.
PF12804. NTP_transf_3. 1 hit.
[Graphical view ]
SUPFAMi SSF51161. SSF51161. 1 hit.
SSF53448. SSF53448. 1 hit.
TIGRFAMsi TIGR01173. glmU. 1 hit.
PROSITEi PS00101. HEXAPEP_TRANSFERASES. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Complete sequence of Anaeromyxobacter dehalogenans 2CP-1."
    Lucas S., Copeland A., Lapidus A., Glavina del Rio T., Dalin E., Tice H., Bruce D., Goodwin L., Pitluck S., Saunders E., Brettin T., Detter J.C., Han C., Larimer F., Land M., Hauser L., Kyrpides N., Ovchinnikova G., Beliaev A.S., Richardson P.
    Submitted (JAN-2009) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 2CP-1 / ATCC BAA-258.

Entry informationi

Entry nameiGLMU_ANAD2
AccessioniPrimary (citable) accession number: B8J9N1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: March 3, 2009
Last modified: October 29, 2014
This is version 40 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Multifunctional enzyme

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3