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B8J893 (TRMD_ANAD2) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 35. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
tRNA (guanine-N(1)-)-methyltransferase

EC=2.1.1.228
Alternative name(s):
M1G-methyltransferase
tRNA [GM37] methyltransferase
Gene names
Name:trmD
Ordered Locus Names:A2cp1_2051
OrganismAnaeromyxobacter dehalogenans (strain 2CP-1 / ATCC BAA-258) [Complete proteome] [HAMAP]
Taxonomic identifier455488 [NCBI]
Taxonomic lineageBacteriaProteobacteriaDeltaproteobacteriaMyxococcalesCystobacterineaeMyxococcaceaeAnaeromyxobacter

Protein attributes

Sequence length247 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Specifically methylates guanosine-37 in various tRNAs By similarity. HAMAP-Rule MF_00605

Catalytic activity

S-adenosyl-L-methionine + guanine37 in tRNA = S-adenosyl-L-homocysteine + N(1)-methylguanine37 in tRNA. HAMAP-Rule MF_00605

Subunit structure

Homodimer By similarity. HAMAP-Rule MF_00605

Subcellular location

Cytoplasm Potential HAMAP-Rule MF_00605.

Sequence similarities

Belongs to the RNA methyltransferase TrmD family.

Ontologies

Keywords
   Biological processtRNA processing
   Cellular componentCytoplasm
   LigandS-adenosyl-L-methionine
   Molecular functionMethyltransferase
Transferase
   Technical termComplete proteome
Gene Ontology (GO)
   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functiontRNA (guanine(37)-N(1))-methyltransferase activity

Inferred from electronic annotation. Source: UniProtKB-EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 247247tRNA (guanine-N(1)-)-methyltransferase HAMAP-Rule MF_00605
PRO_1000147066

Regions

Region134 – 1396S-adenosyl-L-methionine binding By similarity

Sites

Binding site1151S-adenosyl-L-methionine; via amide nitrogen By similarity

Sequences

Sequence LengthMass (Da)Tools
B8J893 [UniParc].

Last modified March 3, 2009. Version 1.
Checksum: 52AACEBD1E761B78

FASTA24726,861
        10         20         30         40         50         60 
MARLEVDILT LFPRMCAGYL GESILGKAQE AGLLAATITD IRDHATGKHR VCDDAPYGGG 

        70         80         90        100        110        120 
AGMVMKPEPL VEAIEAARAR LPGAWVVLTS PRGARLDQAL ARRFAEHGRL ILACGRYEGV 

       130        140        150        160        170        180 
DERVMTAVDM QVSIGDFVLT GGELAALCVV DAAARLVPGV LGNAASADAE SFAGVEGLLE 

       190        200        210        220        230        240 
HPQYTRPPEF RGMKVPEVLL SGDHRRIERW RRREALRATR ERRPDLFARL SLPESDLRLI 


EAGDDEL 

« Hide

References

[1]"Complete sequence of Anaeromyxobacter dehalogenans 2CP-1."
Lucas S., Copeland A., Lapidus A., Glavina del Rio T., Dalin E., Tice H., Bruce D., Goodwin L., Pitluck S., Saunders E., Brettin T., Detter J.C., Han C., Larimer F., Land M., Hauser L., Kyrpides N., Ovchinnikova G., Beliaev A.S., Richardson P.
Submitted (JAN-2009) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: 2CP-1 / ATCC BAA-258.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP001359 Genomic DNA. Translation: ACL65392.1.
RefSeqYP_002492458.1. NC_011891.1.

3D structure databases

ProteinModelPortalB8J893.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING455488.A2cp1_2051.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaACL65392; ACL65392; A2cp1_2051.
GeneID7300178.
KEGGacp:A2cp1_2051.
PATRIC20910990. VBIAnaDeh28364_2085.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0336.
HOGENOMHOG000016242.
KOK00554.
OMACGRFEGI.
OrthoDBEOG6J48RZ.
ProtClustDBPRK00026.

Enzyme and pathway databases

BioCycADEH455488:GH35-2070-MONOMER.

Family and domain databases

Gene3D1.10.1270.20. 1 hit.
HAMAPMF_00605. TrmD.
InterProIPR002649. tRNA_m1G_MeTrfase_bac.
IPR023148. tRNA_m1G_MeTrfase_C.
IPR016009. tRNA_MeTrfase_TRMD/TRM10.
[Graphical view]
PfamPF01746. tRNA_m1G_MT. 1 hit.
[Graphical view]
PIRSFPIRSF000386. tRNA_mtase. 1 hit.
TIGRFAMsTIGR00088. trmD. 1 hit.
ProtoNetSearch...

Entry information

Entry nameTRMD_ANAD2
AccessionPrimary (citable) accession number: B8J893
Entry history
Integrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: March 3, 2009
Last modified: April 16, 2014
This is version 35 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families