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Protein

Phosphoenolpyruvate carboxykinase [GTP]

Gene

pckG

Organism
Anaeromyxobacter dehalogenans (strain 2CP-1 / ATCC BAA-258)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the conversion of oxaloacetate (OAA) to phosphoenolpyruvate (PEP), the rate-limiting step in the metabolic pathway that produces glucose from lactate and other precursors derived from the citric acid cycle.UniRule annotation

Catalytic activityi

GTP + oxaloacetate = GDP + phosphoenolpyruvate + CO2.UniRule annotation

Cofactori

Mn2+UniRule annotationNote: Binds 1 Mn2+ ion per subunit.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei77 – 771SubstrateUniRule annotation
Binding sitei207 – 2071Substrate; via amide nitrogenUniRule annotation
Metal bindingi214 – 2141ManganeseUniRule annotation
Binding sitei214 – 2141SubstrateUniRule annotation
Metal bindingi234 – 2341Manganese; via tele nitrogenUniRule annotation
Binding sitei256 – 2561SubstrateUniRule annotation
Active sitei258 – 2581UniRule annotation
Metal bindingi283 – 2831ManganeseUniRule annotation
Binding sitei375 – 3751GTPUniRule annotation
Binding sitei406 – 4061GTPUniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi257 – 2626GTPUniRule annotation
Nucleotide bindingi499 – 5024GTPUniRule annotation

GO - Molecular functioni

  1. GTP binding Source: UniProtKB-HAMAP
  2. manganese ion binding Source: UniProtKB-HAMAP
  3. phosphoenolpyruvate carboxykinase (GTP) activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. gluconeogenesis Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Decarboxylase, Lyase

Keywords - Biological processi

Gluconeogenesis

Keywords - Ligandi

GTP-binding, Manganese, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciADEH455488:GH35-3871-MONOMER.
UniPathwayiUPA00138.

Names & Taxonomyi

Protein namesi
Recommended name:
Phosphoenolpyruvate carboxykinase [GTP]UniRule annotation (EC:4.1.1.32UniRule annotation)
Short name:
PEP carboxykinaseUniRule annotation
Short name:
PEPCKUniRule annotation
Gene namesi
Name:pckGUniRule annotation
Ordered Locus Names:A2cp1_3817
OrganismiAnaeromyxobacter dehalogenans (strain 2CP-1 / ATCC BAA-258)
Taxonomic identifieri455488 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaDeltaproteobacteriaMyxococcalesCystobacterineaeAnaeromyxobacteraceaeAnaeromyxobacter
ProteomesiUP000007089 Componenti: Chromosome

Subcellular locationi

  1. Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 596596Phosphoenolpyruvate carboxykinase [GTP]PRO_1000192343Add
BLAST

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi455488.A2cp1_3817.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni373 – 3753Substrate bindingUniRule annotation

Sequence similaritiesi

Belongs to the phosphoenolpyruvate carboxykinase [GTP] family.UniRule annotation

Phylogenomic databases

eggNOGiCOG1274.
HOGENOMiHOG000191700.
KOiK01596.
OMAiWMRFGED.
OrthoDBiEOG6MPWQS.

Family and domain databases

Gene3Di3.40.449.10. 1 hit.
3.90.228.20. 2 hits.
HAMAPiMF_00452. PEPCK_GTP.
InterProiIPR018091. PEP_carboxykin_GTP_CS.
IPR013035. PEP_carboxykinase_C.
IPR008209. PEP_carboxykinase_GTP.
IPR008210. PEP_carboxykinase_N.
[Graphical view]
PANTHERiPTHR11561. PTHR11561. 1 hit.
PfamiPF00821. PEPCK. 1 hit.
[Graphical view]
PIRSFiPIRSF001348. PEP_carboxykinase_GTP. 1 hit.
SUPFAMiSSF68923. SSF68923. 1 hit.
PROSITEiPS00505. PEPCK_GTP. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

B8J7G1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSTSPAARPT SNAHLLGWVD EMAKLCKPDR VYWCDGSEAE KKRLTDDAVA
60 70 80 90 100
AKVLIPLDQQ KWPGCHYHHS NSSDVARVEH LTFICTPTKE QAGPTNNWME
110 120 130 140 150
PKEAYRKLGA IFDGSMKGRT MYVVPYVMGP STSPFAKVGI EITDSVYVAL
160 170 180 190 200
NMGIMARMGK VALDRLGDSD EFNRGLHSVA DCNPERRFIC HFPQDNTIWS
210 220 230 240 250
VGSGYGGNAL LGKKCLALRI ASYLAKNEGW LAEHMLILEA ESPTGEKQYV
260 270 280 290 300
AAAFPSACGK TNFAMMIPPA AFPGWKIRTV GDDISWMRVG EDGRLWAVNP
310 320 330 340 350
ENGYFGVAPG TNRKTNPNAM DSVRKDTIFT NVARTPDGDI WWEGMDHEAP
360 370 380 390 400
AELIDWKGQP WKKGSTEKAA HPNSRFTAPA KNNPAISPLV DDPKGVPISA
410 420 430 440 450
IIFGGRRSTT VPLVLEAFNW THGVYLGSTM GSETTAAATG QVGVVRRDPM
460 470 480 490 500
AMLPFIGYDC GSYLQHWLDM QSRIPNPPKI FLVNWFRKSA EGKFLWPGYG
510 520 530 540 550
DNMRVLKWML DRAAGRAPAK ETLLGYTPGD SGLDLHGLDV SKDAIAAATQ
560 570 580 590
IDLGEWEQEL ESQSEWFEKL GKTLPRPLAL QRELLLERVR AARKVK
Length:596
Mass (Da):65,869
Last modified:March 3, 2009 - v1
Checksum:i053D58D526ECC4A9
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001359 Genomic DNA. Translation: ACL67141.1.
RefSeqiWP_015934890.1. NC_011891.1.
YP_002494207.1. NC_011891.1.

Genome annotation databases

EnsemblBacteriaiACL67141; ACL67141; A2cp1_3817.
KEGGiacp:A2cp1_3817.
PATRICi20914591. VBIAnaDeh28364_3857.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001359 Genomic DNA. Translation: ACL67141.1.
RefSeqiWP_015934890.1. NC_011891.1.
YP_002494207.1. NC_011891.1.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi455488.A2cp1_3817.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiACL67141; ACL67141; A2cp1_3817.
KEGGiacp:A2cp1_3817.
PATRICi20914591. VBIAnaDeh28364_3857.

Phylogenomic databases

eggNOGiCOG1274.
HOGENOMiHOG000191700.
KOiK01596.
OMAiWMRFGED.
OrthoDBiEOG6MPWQS.

Enzyme and pathway databases

UniPathwayiUPA00138.
BioCyciADEH455488:GH35-3871-MONOMER.

Family and domain databases

Gene3Di3.40.449.10. 1 hit.
3.90.228.20. 2 hits.
HAMAPiMF_00452. PEPCK_GTP.
InterProiIPR018091. PEP_carboxykin_GTP_CS.
IPR013035. PEP_carboxykinase_C.
IPR008209. PEP_carboxykinase_GTP.
IPR008210. PEP_carboxykinase_N.
[Graphical view]
PANTHERiPTHR11561. PTHR11561. 1 hit.
PfamiPF00821. PEPCK. 1 hit.
[Graphical view]
PIRSFiPIRSF001348. PEP_carboxykinase_GTP. 1 hit.
SUPFAMiSSF68923. SSF68923. 1 hit.
PROSITEiPS00505. PEPCK_GTP. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Complete sequence of Anaeromyxobacter dehalogenans 2CP-1."
    Lucas S., Copeland A., Lapidus A., Glavina del Rio T., Dalin E., Tice H., Bruce D., Goodwin L., Pitluck S., Saunders E., Brettin T., Detter J.C., Han C., Larimer F., Land M., Hauser L., Kyrpides N., Ovchinnikova G., Beliaev A.S., Richardson P.
    Submitted (JAN-2009) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 2CP-1 / ATCC BAA-258.

Entry informationi

Entry nameiPCKG_ANAD2
AccessioniPrimary (citable) accession number: B8J7G1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: March 3, 2009
Last modified: April 29, 2015
This is version 42 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.