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B8J7G1

- PCKG_ANAD2

UniProt

B8J7G1 - PCKG_ANAD2

Protein

Phosphoenolpyruvate carboxykinase [GTP]

Gene

pckG

Organism
Anaeromyxobacter dehalogenans (strain 2CP-1 / ATCC BAA-258)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 35 (01 Oct 2014)
      Sequence version 1 (03 Mar 2009)
      Previous versions | rss
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    Functioni

    Catalyzes the conversion of oxaloacetate (OAA) to phosphoenolpyruvate (PEP), the rate-limiting step in the metabolic pathway that produces glucose from lactate and other precursors derived from the citric acid cycle.UniRule annotation

    Catalytic activityi

    GTP + oxaloacetate = GDP + phosphoenolpyruvate + CO2.UniRule annotation

    Cofactori

    Binds 1 manganese ion per subunit.UniRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei77 – 771SubstrateUniRule annotation
    Binding sitei207 – 2071Substrate; via amide nitrogenUniRule annotation
    Metal bindingi214 – 2141ManganeseUniRule annotation
    Binding sitei214 – 2141SubstrateUniRule annotation
    Metal bindingi234 – 2341Manganese; via tele nitrogenUniRule annotation
    Binding sitei256 – 2561SubstrateUniRule annotation
    Active sitei258 – 2581UniRule annotation
    Metal bindingi283 – 2831ManganeseUniRule annotation
    Binding sitei375 – 3751GTPUniRule annotation
    Binding sitei406 – 4061GTPUniRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi257 – 2626GTPUniRule annotation
    Nucleotide bindingi499 – 5024GTPUniRule annotation

    GO - Molecular functioni

    1. GTP binding Source: UniProtKB-HAMAP
    2. manganese ion binding Source: UniProtKB-HAMAP
    3. phosphoenolpyruvate carboxykinase (GTP) activity Source: UniProtKB-HAMAP

    GO - Biological processi

    1. gluconeogenesis Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Decarboxylase, Lyase

    Keywords - Biological processi

    Gluconeogenesis

    Keywords - Ligandi

    GTP-binding, Manganese, Metal-binding, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciADEH455488:GH35-3871-MONOMER.
    UniPathwayiUPA00138.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Phosphoenolpyruvate carboxykinase [GTP]UniRule annotation (EC:4.1.1.32UniRule annotation)
    Short name:
    PEP carboxykinaseUniRule annotation
    Short name:
    PEPCKUniRule annotation
    Gene namesi
    Name:pckGUniRule annotation
    Ordered Locus Names:A2cp1_3817
    OrganismiAnaeromyxobacter dehalogenans (strain 2CP-1 / ATCC BAA-258)
    Taxonomic identifieri455488 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaDeltaproteobacteriaMyxococcalesCystobacterineaeAnaeromyxobacteraceaeAnaeromyxobacter
    ProteomesiUP000007089: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 596596Phosphoenolpyruvate carboxykinase [GTP]PRO_1000192343Add
    BLAST

    Interactioni

    Subunit structurei

    Monomer.UniRule annotation

    Protein-protein interaction databases

    STRINGi455488.A2cp1_3817.

    Structurei

    3D structure databases

    ProteinModelPortaliB8J7G1.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni373 – 3753Substrate bindingUniRule annotation

    Sequence similaritiesi

    Belongs to the phosphoenolpyruvate carboxykinase [GTP] family.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG1274.
    HOGENOMiHOG000191700.
    KOiK01596.
    OMAiWIELGEN.
    OrthoDBiEOG6MPWQS.

    Family and domain databases

    Gene3Di3.40.449.10. 1 hit.
    3.90.228.20. 2 hits.
    HAMAPiMF_00452. PEPCK_GTP.
    InterProiIPR018091. PEP_carboxykin_GTP_CS.
    IPR013035. PEP_carboxykinase_C.
    IPR008209. PEP_carboxykinase_GTP.
    IPR008210. PEP_carboxykinase_N.
    [Graphical view]
    PANTHERiPTHR11561. PTHR11561. 1 hit.
    PfamiPF00821. PEPCK. 1 hit.
    [Graphical view]
    PIRSFiPIRSF001348. PEP_carboxykinase_GTP. 1 hit.
    SUPFAMiSSF68923. SSF68923. 1 hit.
    PROSITEiPS00505. PEPCK_GTP. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    B8J7G1-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSTSPAARPT SNAHLLGWVD EMAKLCKPDR VYWCDGSEAE KKRLTDDAVA    50
    AKVLIPLDQQ KWPGCHYHHS NSSDVARVEH LTFICTPTKE QAGPTNNWME 100
    PKEAYRKLGA IFDGSMKGRT MYVVPYVMGP STSPFAKVGI EITDSVYVAL 150
    NMGIMARMGK VALDRLGDSD EFNRGLHSVA DCNPERRFIC HFPQDNTIWS 200
    VGSGYGGNAL LGKKCLALRI ASYLAKNEGW LAEHMLILEA ESPTGEKQYV 250
    AAAFPSACGK TNFAMMIPPA AFPGWKIRTV GDDISWMRVG EDGRLWAVNP 300
    ENGYFGVAPG TNRKTNPNAM DSVRKDTIFT NVARTPDGDI WWEGMDHEAP 350
    AELIDWKGQP WKKGSTEKAA HPNSRFTAPA KNNPAISPLV DDPKGVPISA 400
    IIFGGRRSTT VPLVLEAFNW THGVYLGSTM GSETTAAATG QVGVVRRDPM 450
    AMLPFIGYDC GSYLQHWLDM QSRIPNPPKI FLVNWFRKSA EGKFLWPGYG 500
    DNMRVLKWML DRAAGRAPAK ETLLGYTPGD SGLDLHGLDV SKDAIAAATQ 550
    IDLGEWEQEL ESQSEWFEKL GKTLPRPLAL QRELLLERVR AARKVK 596
    Length:596
    Mass (Da):65,869
    Last modified:March 3, 2009 - v1
    Checksum:i053D58D526ECC4A9
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP001359 Genomic DNA. Translation: ACL67141.1.
    RefSeqiWP_015934890.1. NC_011891.1.
    YP_002494207.1. NC_011891.1.

    Genome annotation databases

    EnsemblBacteriaiACL67141; ACL67141; A2cp1_3817.
    GeneIDi7296193.
    KEGGiacp:A2cp1_3817.
    PATRICi20914591. VBIAnaDeh28364_3857.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP001359 Genomic DNA. Translation: ACL67141.1 .
    RefSeqi WP_015934890.1. NC_011891.1.
    YP_002494207.1. NC_011891.1.

    3D structure databases

    ProteinModelPortali B8J7G1.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 455488.A2cp1_3817.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai ACL67141 ; ACL67141 ; A2cp1_3817 .
    GeneIDi 7296193.
    KEGGi acp:A2cp1_3817.
    PATRICi 20914591. VBIAnaDeh28364_3857.

    Phylogenomic databases

    eggNOGi COG1274.
    HOGENOMi HOG000191700.
    KOi K01596.
    OMAi WIELGEN.
    OrthoDBi EOG6MPWQS.

    Enzyme and pathway databases

    UniPathwayi UPA00138 .
    BioCyci ADEH455488:GH35-3871-MONOMER.

    Family and domain databases

    Gene3Di 3.40.449.10. 1 hit.
    3.90.228.20. 2 hits.
    HAMAPi MF_00452. PEPCK_GTP.
    InterProi IPR018091. PEP_carboxykin_GTP_CS.
    IPR013035. PEP_carboxykinase_C.
    IPR008209. PEP_carboxykinase_GTP.
    IPR008210. PEP_carboxykinase_N.
    [Graphical view ]
    PANTHERi PTHR11561. PTHR11561. 1 hit.
    Pfami PF00821. PEPCK. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF001348. PEP_carboxykinase_GTP. 1 hit.
    SUPFAMi SSF68923. SSF68923. 1 hit.
    PROSITEi PS00505. PEPCK_GTP. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Complete sequence of Anaeromyxobacter dehalogenans 2CP-1."
      Lucas S., Copeland A., Lapidus A., Glavina del Rio T., Dalin E., Tice H., Bruce D., Goodwin L., Pitluck S., Saunders E., Brettin T., Detter J.C., Han C., Larimer F., Land M., Hauser L., Kyrpides N., Ovchinnikova G., Beliaev A.S., Richardson P.
      Submitted (JAN-2009) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: 2CP-1 / ATCC BAA-258.

    Entry informationi

    Entry nameiPCKG_ANAD2
    AccessioniPrimary (citable) accession number: B8J7G1
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 28, 2009
    Last sequence update: March 3, 2009
    Last modified: October 1, 2014
    This is version 35 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3