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B8J7G1

- PCKG_ANAD2

UniProt

B8J7G1 - PCKG_ANAD2

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Protein

Phosphoenolpyruvate carboxykinase [GTP]

Gene

pckG

Organism
Anaeromyxobacter dehalogenans (strain 2CP-1 / ATCC BAA-258)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Catalyzes the conversion of oxaloacetate (OAA) to phosphoenolpyruvate (PEP), the rate-limiting step in the metabolic pathway that produces glucose from lactate and other precursors derived from the citric acid cycle.UniRule annotation

Catalytic activityi

GTP + oxaloacetate = GDP + phosphoenolpyruvate + CO2.UniRule annotation

Cofactori

Binds 1 manganese ion per subunit.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei77 – 771SubstrateUniRule annotation
Binding sitei207 – 2071Substrate; via amide nitrogenUniRule annotation
Metal bindingi214 – 2141ManganeseUniRule annotation
Binding sitei214 – 2141SubstrateUniRule annotation
Metal bindingi234 – 2341Manganese; via tele nitrogenUniRule annotation
Binding sitei256 – 2561SubstrateUniRule annotation
Active sitei258 – 2581UniRule annotation
Metal bindingi283 – 2831ManganeseUniRule annotation
Binding sitei375 – 3751GTPUniRule annotation
Binding sitei406 – 4061GTPUniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi257 – 2626GTPUniRule annotation
Nucleotide bindingi499 – 5024GTPUniRule annotation

GO - Molecular functioni

  1. GTP binding Source: UniProtKB-HAMAP
  2. manganese ion binding Source: UniProtKB-HAMAP
  3. phosphoenolpyruvate carboxykinase (GTP) activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. gluconeogenesis Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Decarboxylase, Lyase

Keywords - Biological processi

Gluconeogenesis

Keywords - Ligandi

GTP-binding, Manganese, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciADEH455488:GH35-3871-MONOMER.
UniPathwayiUPA00138.

Names & Taxonomyi

Protein namesi
Recommended name:
Phosphoenolpyruvate carboxykinase [GTP]UniRule annotation (EC:4.1.1.32UniRule annotation)
Short name:
PEP carboxykinaseUniRule annotation
Short name:
PEPCKUniRule annotation
Gene namesi
Name:pckGUniRule annotation
Ordered Locus Names:A2cp1_3817
OrganismiAnaeromyxobacter dehalogenans (strain 2CP-1 / ATCC BAA-258)
Taxonomic identifieri455488 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaDeltaproteobacteriaMyxococcalesCystobacterineaeAnaeromyxobacteraceaeAnaeromyxobacter
ProteomesiUP000007089: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 596596Phosphoenolpyruvate carboxykinase [GTP]PRO_1000192343Add
BLAST

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi455488.A2cp1_3817.

Structurei

3D structure databases

ProteinModelPortaliB8J7G1.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni373 – 3753Substrate bindingUniRule annotation

Sequence similaritiesi

Belongs to the phosphoenolpyruvate carboxykinase [GTP] family.UniRule annotation

Phylogenomic databases

eggNOGiCOG1274.
HOGENOMiHOG000191700.
KOiK01596.
OMAiWIELGEN.
OrthoDBiEOG6MPWQS.

Family and domain databases

Gene3Di3.40.449.10. 1 hit.
3.90.228.20. 2 hits.
HAMAPiMF_00452. PEPCK_GTP.
InterProiIPR018091. PEP_carboxykin_GTP_CS.
IPR013035. PEP_carboxykinase_C.
IPR008209. PEP_carboxykinase_GTP.
IPR008210. PEP_carboxykinase_N.
[Graphical view]
PANTHERiPTHR11561. PTHR11561. 1 hit.
PfamiPF00821. PEPCK. 1 hit.
[Graphical view]
PIRSFiPIRSF001348. PEP_carboxykinase_GTP. 1 hit.
SUPFAMiSSF68923. SSF68923. 1 hit.
PROSITEiPS00505. PEPCK_GTP. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

B8J7G1-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MSTSPAARPT SNAHLLGWVD EMAKLCKPDR VYWCDGSEAE KKRLTDDAVA
60 70 80 90 100
AKVLIPLDQQ KWPGCHYHHS NSSDVARVEH LTFICTPTKE QAGPTNNWME
110 120 130 140 150
PKEAYRKLGA IFDGSMKGRT MYVVPYVMGP STSPFAKVGI EITDSVYVAL
160 170 180 190 200
NMGIMARMGK VALDRLGDSD EFNRGLHSVA DCNPERRFIC HFPQDNTIWS
210 220 230 240 250
VGSGYGGNAL LGKKCLALRI ASYLAKNEGW LAEHMLILEA ESPTGEKQYV
260 270 280 290 300
AAAFPSACGK TNFAMMIPPA AFPGWKIRTV GDDISWMRVG EDGRLWAVNP
310 320 330 340 350
ENGYFGVAPG TNRKTNPNAM DSVRKDTIFT NVARTPDGDI WWEGMDHEAP
360 370 380 390 400
AELIDWKGQP WKKGSTEKAA HPNSRFTAPA KNNPAISPLV DDPKGVPISA
410 420 430 440 450
IIFGGRRSTT VPLVLEAFNW THGVYLGSTM GSETTAAATG QVGVVRRDPM
460 470 480 490 500
AMLPFIGYDC GSYLQHWLDM QSRIPNPPKI FLVNWFRKSA EGKFLWPGYG
510 520 530 540 550
DNMRVLKWML DRAAGRAPAK ETLLGYTPGD SGLDLHGLDV SKDAIAAATQ
560 570 580 590
IDLGEWEQEL ESQSEWFEKL GKTLPRPLAL QRELLLERVR AARKVK
Length:596
Mass (Da):65,869
Last modified:March 3, 2009 - v1
Checksum:i053D58D526ECC4A9
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP001359 Genomic DNA. Translation: ACL67141.1.
RefSeqiYP_002494207.1. NC_011891.1.

Genome annotation databases

EnsemblBacteriaiACL67141; ACL67141; A2cp1_3817.
GeneIDi7296193.
KEGGiacp:A2cp1_3817.
PATRICi20914591. VBIAnaDeh28364_3857.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP001359 Genomic DNA. Translation: ACL67141.1 .
RefSeqi YP_002494207.1. NC_011891.1.

3D structure databases

ProteinModelPortali B8J7G1.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 455488.A2cp1_3817.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ACL67141 ; ACL67141 ; A2cp1_3817 .
GeneIDi 7296193.
KEGGi acp:A2cp1_3817.
PATRICi 20914591. VBIAnaDeh28364_3857.

Phylogenomic databases

eggNOGi COG1274.
HOGENOMi HOG000191700.
KOi K01596.
OMAi WIELGEN.
OrthoDBi EOG6MPWQS.

Enzyme and pathway databases

UniPathwayi UPA00138 .
BioCyci ADEH455488:GH35-3871-MONOMER.

Family and domain databases

Gene3Di 3.40.449.10. 1 hit.
3.90.228.20. 2 hits.
HAMAPi MF_00452. PEPCK_GTP.
InterProi IPR018091. PEP_carboxykin_GTP_CS.
IPR013035. PEP_carboxykinase_C.
IPR008209. PEP_carboxykinase_GTP.
IPR008210. PEP_carboxykinase_N.
[Graphical view ]
PANTHERi PTHR11561. PTHR11561. 1 hit.
Pfami PF00821. PEPCK. 1 hit.
[Graphical view ]
PIRSFi PIRSF001348. PEP_carboxykinase_GTP. 1 hit.
SUPFAMi SSF68923. SSF68923. 1 hit.
PROSITEi PS00505. PEPCK_GTP. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Complete sequence of Anaeromyxobacter dehalogenans 2CP-1."
    Lucas S., Copeland A., Lapidus A., Glavina del Rio T., Dalin E., Tice H., Bruce D., Goodwin L., Pitluck S., Saunders E., Brettin T., Detter J.C., Han C., Larimer F., Land M., Hauser L., Kyrpides N., Ovchinnikova G., Beliaev A.S., Richardson P.
    Submitted (JAN-2009) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 2CP-1 / ATCC BAA-258.

Entry informationi

Entry nameiPCKG_ANAD2
AccessioniPrimary (citable) accession number: B8J7G1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: March 3, 2009
Last modified: October 29, 2014
This is version 36 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3