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Protein

Glutamyl-tRNA(Gln) amidotransferase subunit A

Gene

gatA

Organism
Desulfovibrio desulfuricans (strain ATCC 27774 / DSM 6949)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Allows the formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu-tRNA(Gln) in organisms which lack glutaminyl-tRNA synthetase. The reaction takes place in the presence of glutamine and ATP through an activated gamma-phospho-Glu-tRNA(Gln).UniRule annotation

Catalytic activityi

ATP + L-glutamyl-tRNA(Gln) + L-glutamine = ADP + phosphate + L-glutaminyl-tRNA(Gln) + L-glutamate.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei78Charge relay systemUniRule annotation1
Active sitei153Charge relay systemUniRule annotation1
Active sitei177Acyl-ester intermediateUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Glutamyl-tRNA(Gln) amidotransferase subunit AUniRule annotation (EC:6.3.5.7UniRule annotation)
Short name:
Glu-ADT subunit AUniRule annotation
Gene namesi
Name:gatAUniRule annotation
Ordered Locus Names:Ddes_2154
OrganismiDesulfovibrio desulfuricans (strain ATCC 27774 / DSM 6949)
Taxonomic identifieri525146 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaDeltaproteobacteriaDesulfovibrionalesDesulfovibrionaceaeDesulfovibrio
Proteomesi
  • UP000002598 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_10001224781 – 486Glutamyl-tRNA(Gln) amidotransferase subunit AAdd BLAST486

Interactioni

Subunit structurei

Heterotrimer of A, B and C subunits.UniRule annotation

Protein-protein interaction databases

STRINGi525146.Ddes_2154.

Family & Domainsi

Sequence similaritiesi

Belongs to the amidase family. GatA subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105C3P. Bacteria.
COG0154. LUCA.
HOGENOMiHOG000116699.
KOiK02433.
OMAiKEYFGAG.
OrthoDBiPOG091H00O9.

Family and domain databases

Gene3Di3.90.1300.10. 1 hit.
HAMAPiMF_00120. GatA. 1 hit.
InterProiIPR000120. Amidase.
IPR023631. Amidase_dom.
IPR004412. GatA.
[Graphical view]
PANTHERiPTHR11895. PTHR11895. 1 hit.
PfamiPF01425. Amidase. 1 hit.
[Graphical view]
SUPFAMiSSF75304. SSF75304. 1 hit.
TIGRFAMsiTIGR00132. gatA. 1 hit.

Sequencei

Sequence statusi: Complete.

B8J405-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTDICSLSLT AVAQALQKKE LSAVDVTTAC LDRITATEPH LAALLYVNAE
60 70 80 90 100
NALARATTLD SEGPDAARPL WGVPVTLKDA FSTRGMPTTA GSRMLEGYTP
110 120 130 140 150
FYDAFAVQKL HEAGAVILGK TNLDEFAMGS STENSAFKVT RNPWNLNKVP
160 170 180 190 200
GGSSGGSAAS VTAGQCFASL GTDTGGSIRQ PASFCGCVGL KPTYGRVSRY
210 220 230 240 250
GVIAYGSSLD QVGPLTRSVE DCARVLTAIA GYDSRDNTCD PRPAEDYAAT
260 270 280 290 300
LSSRPLKGAR LGIPREFYGQ GLSDEVRAAC EAAIQAARNQ DAELVEVSLP
310 320 330 340 350
HTDAAIATYY IIAMAEASSN LARFDGVRFG HRSADIKNLD DLYVHSRTEG
360 370 380 390 400
FGPEVKRRIM LGAYVLSSGY YDAYYRKAAQ VRRLIRDELL AALGQCDALL
410 420 430 440 450
APVSPVTARD LGGNTADPLQ IYLMDAYTLS LNLAGLPGLS LPVGLGAKSG
460 470 480
MPVGMQIIGK PFEETQVLAL GHSLEQALPG IGRPAL
Length:486
Mass (Da):51,368
Last modified:March 3, 2009 - v1
Checksum:i5F7AE6592220810E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001358 Genomic DNA. Translation: ACL50050.1.
RefSeqiWP_015939159.1. NC_011883.1.

Genome annotation databases

EnsemblBacteriaiACL50050; ACL50050; Ddes_2154.
KEGGidds:Ddes_2154.
PATRICi21738366. VBIDesDes50650_2515.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001358 Genomic DNA. Translation: ACL50050.1.
RefSeqiWP_015939159.1. NC_011883.1.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi525146.Ddes_2154.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiACL50050; ACL50050; Ddes_2154.
KEGGidds:Ddes_2154.
PATRICi21738366. VBIDesDes50650_2515.

Phylogenomic databases

eggNOGiENOG4105C3P. Bacteria.
COG0154. LUCA.
HOGENOMiHOG000116699.
KOiK02433.
OMAiKEYFGAG.
OrthoDBiPOG091H00O9.

Family and domain databases

Gene3Di3.90.1300.10. 1 hit.
HAMAPiMF_00120. GatA. 1 hit.
InterProiIPR000120. Amidase.
IPR023631. Amidase_dom.
IPR004412. GatA.
[Graphical view]
PANTHERiPTHR11895. PTHR11895. 1 hit.
PfamiPF01425. Amidase. 1 hit.
[Graphical view]
SUPFAMiSSF75304. SSF75304. 1 hit.
TIGRFAMsiTIGR00132. gatA. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiGATA_DESDA
AccessioniPrimary (citable) accession number: B8J405
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: March 3, 2009
Last modified: November 2, 2016
This is version 43 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.