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B8J3Y7 (B8J3Y7_DESDA) Unreviewed, UniProtKB/TrEMBL

Last modified June 11, 2014. Version 38. Feed History...

Clusters with 100%, 90%, 50% identity | Third-party data text xml rdf/xml gff fasta
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Protein attributes

Sequence length404 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the conversion of D-ribulose 5-phosphate to formate and 3,4-dihydroxy-2-butanone 4-phosphate By similarity. SAAS SAAS017945 HAMAP-Rule MF_01283

Catalyzes the conversion of GTP to 2,5-diamino-6-ribosylamino-4(3H)-pyrimidinone 5'-phosphate (DARP), formate and pyrophosphate By similarity. SAAS SAAS017945 HAMAP-Rule MF_01283

Catalytic activity

D-ribulose 5-phosphate = formate + L-3,4-dihydroxybutan-2-one 4-phosphate. HAMAP-Rule MF_01283

GTP + 3 H2O = formate + 2,5-diamino-6-hydroxy-4-(5-phospho-D-ribosylamino)pyrimidine + diphosphate. SAAS SAAS016299 HAMAP-Rule MF_01283

Cofactor

Binds 1 zinc ion per subunit By similarity. SAAS SAAS016299 HAMAP-Rule MF_01283

Binds 2 divalent metal cations per subunit. Magnesium or manganese By similarity. HAMAP-Rule MF_01283

Pathway

Cofactor biosynthesis; riboflavin biosynthesis; 2-hydroxy-3-oxobutyl phosphate from D-ribulose 5-phosphate: step 1/1. HAMAP-Rule MF_01283

Cofactor biosynthesis; riboflavin biosynthesis; 5-amino-6-(D-ribitylamino)uracil from GTP: step 1/4. SAAS SAAS016299 HAMAP-Rule MF_01283

Sequence similarities

In the C-terminal section; belongs to the GTP cyclohydrolase II family. HAMAP-Rule MF_01283

In the N-terminal section; belongs to the DHBP synthase family. HAMAP-Rule MF_01283

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Regions

Nucleotide binding253 – 2575GTP By similarity HAMAP-Rule MF_01283
Nucleotide binding296 – 2983GTP By similarity HAMAP-Rule MF_01283
Region1 – 201201DHBP synthase By similarity HAMAP-Rule MF_01283
Region27 – 282D-ribulose 5-phosphate binding By similarity HAMAP-Rule MF_01283
Region202 – 404203GTP cyclohydrolase II By similarity HAMAP-Rule MF_01283

Sites

Active site3301Proton acceptor; for GTP cyclohydrolase activity By similarity HAMAP-Rule MF_01283
Active site3321Nucleophile; for GTP cyclohydrolase activity By similarity HAMAP-Rule MF_01283
Metal binding281Magnesium or manganese 1 By similarity HAMAP-Rule MF_01283
Metal binding281Magnesium or manganese 2 By similarity HAMAP-Rule MF_01283
Metal binding2581Zinc; catalytic By similarity HAMAP-Rule MF_01283
Metal binding2691Zinc; catalytic By similarity HAMAP-Rule MF_01283
Metal binding2711Zinc; catalytic By similarity HAMAP-Rule MF_01283
Binding site321D-ribulose 5-phosphate By similarity HAMAP-Rule MF_01283
Binding site1641D-ribulose 5-phosphate By similarity HAMAP-Rule MF_01283
Binding site2741GTP By similarity HAMAP-Rule MF_01283
Binding site3181GTP By similarity HAMAP-Rule MF_01283
Binding site3531GTP By similarity HAMAP-Rule MF_01283
Binding site3581GTP By similarity HAMAP-Rule MF_01283
Site1261Essential for DHBP synthase activity By similarity HAMAP-Rule MF_01283
Site1641Essential for DHBP synthase activity By similarity HAMAP-Rule MF_01283

Sequences

Sequence LengthMass (Da)Tools
B8J3Y7 [UniParc].

Last modified March 3, 2009. Version 1.
Checksum: D4526371C276D7E5

FASTA40444,044
        10         20         30         40         50         60 
MPLCSIEEAV ADIKQGKMVI LVDDEGRENE GDLTMAAEFV TPEAINFMAK YGRGLICLPM 

        70         80         90        100        110        120 
SPEMIDRLDL PLMTQRNGSR FGTNFTISIE AREGVTTGIS AADRATTILA AVGDDVRPDD 

       130        140        150        160        170        180 
LVTPGHVFPL RAKAGGVLSR AGQTEGGVDL SKLAGLKPAS VICEIMRDDG TMARMPDLET 

       190        200        210        220        230        240 
FAEEHGLKIV AVKDIIRHRL NRGQVSVRNV AKAHLPTRFG DFTVYAYESE TEQGTHLALV 

       250        260        270        280        290        300 
KGDLSGPEPV LARVHSECLT GDALGSLRCD CGGQLGAALR QIEKEGRGAL LYMRQEGRGI 

       310        320        330        340        350        360 
GLANKIRAYA LQDEGYDTVE ANRKLGFPPD LRDYGTGAQM LVDLGIHKVR LLTNNPKKIV 

       370        380        390        400 
GLSGYGIEIV ERVPIEMDAC PENEHYLRTK KEKMQHMLHC SCCR 

« Hide

References

[1]"Complete sequence of Desulfovibrio desulfuricans subsp. desulfuricans str. ATCC 27774."
US DOE Joint Genome Institute
Lucas S., Copeland A., Lapidus A., Glavina del Rio T., Tice H., Bruce D., Goodwin L., Pitluck S., Sims D., Lu M., Kiss H., Meineke L., Brettin T., Detter J.C., Han C., Larimer F., Land M., Hauser L. expand/collapse author list , Kyrpides N., Ovchinnikova G., Hazen T.C.
Submitted (JAN-2009) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 27774 / DSM 6949.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP001358 Genomic DNA. Translation: ACL50032.1.
RefSeqYP_002480710.1. NC_011883.1.

3D structure databases

ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING525146.Ddes_2136.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaACL50032; ACL50032; Ddes_2136.
GeneID7285852.
KEGGdds:Ddes_2136.
PATRIC21738316. VBIDesDes50650_2490.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0108.
HOGENOMHOG000115440.
KOK14652.
OrthoDBEOG679TK8.

Enzyme and pathway databases

BioCycDDES525146:GIWF-2202-MONOMER.
UniPathwayUPA00275; UER00399.
UPA00275; UER00400.

Family and domain databases

Gene3D3.90.870.10. 1 hit.
HAMAPMF_00179. RibA.
MF_00180. RibB.
MF_01283. RibBA.
InterProIPR017945. DHBP_synth_RibB-like_a/b_dom.
IPR000422. DHBP_synthase_RibB.
IPR000926. GTP_CycHdrlaseII_RibA.
IPR016299. Riboflavin_synth_RibBA.
[Graphical view]
PfamPF00926. DHBP_synthase. 1 hit.
PF00925. GTP_cyclohydro2. 1 hit.
[Graphical view]
PIRSFPIRSF001259. RibA. 1 hit.
SUPFAMSSF55821. SSF55821. 1 hit.
TIGRFAMsTIGR00505. ribA. 1 hit.
TIGR00506. ribB. 1 hit.
ProtoNetSearch...

Entry information

Entry nameB8J3Y7_DESDA
AccessionPrimary (citable) accession number: B8J3Y7
Entry history
Integrated into UniProtKB/TrEMBL: March 3, 2009
Last sequence update: March 3, 2009
Last modified: June 11, 2014
This is version 38 of the entry and version 1 of the sequence. [Complete history]
Entry statusUnreviewed (UniProtKB/TrEMBL)