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B8J3Y7

- B8J3Y7_DESDA

UniProt

B8J3Y7 - B8J3Y7_DESDA

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Protein

Riboflavin biosynthesis protein RibBA

Gene
ribBA, Ddes_2136
Organism
Desulfovibrio desulfuricans (strain ATCC 27774 / DSM 6949)
Status
Unreviewed - Annotation score: 4 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes the conversion of D-ribulose 5-phosphate to formate and 3,4-dihydroxy-2-butanone 4-phosphate By similarity.UniRule annotationSAAS annotations
Catalyzes the conversion of GTP to 2,5-diamino-6-ribosylamino-4(3H)-pyrimidinone 5'-phosphate (DARP), formate and pyrophosphate By similarity.UniRule annotationSAAS annotations

Catalytic activityi

D-ribulose 5-phosphate = formate + L-3,4-dihydroxybutan-2-one 4-phosphate.UniRule annotation
GTP + 3 H2O = formate + 2,5-diamino-6-hydroxy-4-(5-phospho-D-ribosylamino)pyrimidine + diphosphate.UniRule annotationSAAS annotations

Cofactori

Binds 1 zinc ion per subunit By similarity.UniRule annotationSAAS annotations
Binds 2 divalent metal cations per subunit. Magnesium or manganese By similarity.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi28 – 281Magnesium or manganese 1 By similarityUniRule annotation
Metal bindingi28 – 281Magnesium or manganese 2 By similarityUniRule annotation
Binding sitei32 – 321D-ribulose 5-phosphate By similarityUniRule annotation
Sitei126 – 1261Essential for DHBP synthase activity By similarityUniRule annotation
Binding sitei164 – 1641D-ribulose 5-phosphate By similarityUniRule annotation
Sitei164 – 1641Essential for DHBP synthase activity By similarityUniRule annotation
Metal bindingi258 – 2581Zinc; catalytic By similarityUniRule annotation
Metal bindingi269 – 2691Zinc; catalytic By similarityUniRule annotation
Metal bindingi271 – 2711Zinc; catalytic By similarityUniRule annotation
Binding sitei274 – 2741GTP By similarityUniRule annotation
Binding sitei318 – 3181GTP By similarityUniRule annotation
Active sitei330 – 3301Proton acceptor; for GTP cyclohydrolase activity By similarityUniRule annotation
Active sitei332 – 3321Nucleophile; for GTP cyclohydrolase activity By similarityUniRule annotation
Binding sitei353 – 3531GTP By similarityUniRule annotation
Binding sitei358 – 3581GTP By similarityUniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi253 – 2575GTP By similarityUniRule annotation
Nucleotide bindingi296 – 2983GTP By similarityUniRule annotation

GO - Molecular functioni

  1. 3,4-dihydroxy-2-butanone-4-phosphate synthase activity Source: UniProtKB-HAMAP
  2. GTP binding Source: UniProtKB-KW
  3. GTP cyclohydrolase II activity Source: UniProtKB-HAMAP
  4. magnesium ion binding Source: UniProtKB-HAMAP
  5. manganese ion binding Source: UniProtKB-HAMAP
  6. zinc ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. riboflavin biosynthetic process Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

HydrolaseUniRule annotationImported, LyaseUniRule annotation

Keywords - Biological processi

Riboflavin biosynthesisUniRule annotationSAAS annotations

Keywords - Ligandi

GTP-bindingUniRule annotationSAAS annotations, MagnesiumUniRule annotation, ManganeseUniRule annotation, Metal-bindingUniRule annotationSAAS annotations, Nucleotide-binding, ZincUniRule annotationSAAS annotations

Enzyme and pathway databases

BioCyciDDES525146:GIWF-2202-MONOMER.
UniPathwayiUPA00275; UER00399.
UPA00275; UER00400.

Names & Taxonomyi

Protein namesi
Recommended name:
Riboflavin biosynthesis protein RibBAUniRule annotation
Gene namesi
Name:ribBAUniRule annotation
Ordered Locus Names:Ddes_2136Imported
OrganismiDesulfovibrio desulfuricans (strain ATCC 27774 / DSM 6949)Imported
Taxonomic identifieri525146 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaDeltaproteobacteriaDesulfovibrionalesDesulfovibrionaceaeDesulfovibrio
ProteomesiUP000002598: Chromosome

Interactioni

Protein-protein interaction databases

STRINGi525146.Ddes_2136.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 201201DHBP synthase By similarityUniRule annotationAdd
BLAST
Regioni27 – 282D-ribulose 5-phosphate binding By similarityUniRule annotation
Regioni202 – 404203GTP cyclohydrolase II By similarityUniRule annotationAdd
BLAST

Sequence similaritiesi

In the C-terminal section; belongs to the GTP cyclohydrolase II family.UniRule annotation
In the N-terminal section; belongs to the DHBP synthase family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0108.
HOGENOMiHOG000115440.
KOiK14652.
OrthoDBiEOG679TK8.

Family and domain databases

Gene3Di3.90.870.10. 1 hit.
HAMAPiMF_00179. RibA.
MF_00180. RibB.
MF_01283. RibBA.
InterProiIPR017945. DHBP_synth_RibB-like_a/b_dom.
IPR000422. DHBP_synthase_RibB.
IPR000926. GTP_CycHdrlaseII_RibA.
IPR016299. Riboflavin_synth_RibBA.
[Graphical view]
PfamiPF00926. DHBP_synthase. 1 hit.
PF00925. GTP_cyclohydro2. 1 hit.
[Graphical view]
PIRSFiPIRSF001259. RibA. 1 hit.
SUPFAMiSSF55821. SSF55821. 1 hit.
TIGRFAMsiTIGR00505. ribA. 1 hit.
TIGR00506. ribB. 1 hit.

Sequencei

Sequence statusi: Complete.

B8J3Y7-1 [UniParc]FASTAAdd to Basket

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MPLCSIEEAV ADIKQGKMVI LVDDEGRENE GDLTMAAEFV TPEAINFMAK    50
YGRGLICLPM SPEMIDRLDL PLMTQRNGSR FGTNFTISIE AREGVTTGIS 100
AADRATTILA AVGDDVRPDD LVTPGHVFPL RAKAGGVLSR AGQTEGGVDL 150
SKLAGLKPAS VICEIMRDDG TMARMPDLET FAEEHGLKIV AVKDIIRHRL 200
NRGQVSVRNV AKAHLPTRFG DFTVYAYESE TEQGTHLALV KGDLSGPEPV 250
LARVHSECLT GDALGSLRCD CGGQLGAALR QIEKEGRGAL LYMRQEGRGI 300
GLANKIRAYA LQDEGYDTVE ANRKLGFPPD LRDYGTGAQM LVDLGIHKVR 350
LLTNNPKKIV GLSGYGIEIV ERVPIEMDAC PENEHYLRTK KEKMQHMLHC 400
SCCR 404
Length:404
Mass (Da):44,044
Last modified:March 3, 2009 - v1
Checksum:iD4526371C276D7E5
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP001358 Genomic DNA. Translation: ACL50032.1.
RefSeqiYP_002480710.1. NC_011883.1.

Genome annotation databases

EnsemblBacteriaiACL50032; ACL50032; Ddes_2136.
GeneIDi7285852.
KEGGidds:Ddes_2136.
PATRICi21738316. VBIDesDes50650_2490.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP001358 Genomic DNA. Translation: ACL50032.1 .
RefSeqi YP_002480710.1. NC_011883.1.

3D structure databases

ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 525146.Ddes_2136.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ACL50032 ; ACL50032 ; Ddes_2136 .
GeneIDi 7285852.
KEGGi dds:Ddes_2136.
PATRICi 21738316. VBIDesDes50650_2490.

Phylogenomic databases

eggNOGi COG0108.
HOGENOMi HOG000115440.
KOi K14652.
OrthoDBi EOG679TK8.

Enzyme and pathway databases

UniPathwayi UPA00275 ; UER00399 .
UPA00275 ; UER00400 .
BioCyci DDES525146:GIWF-2202-MONOMER.

Family and domain databases

Gene3Di 3.90.870.10. 1 hit.
HAMAPi MF_00179. RibA.
MF_00180. RibB.
MF_01283. RibBA.
InterProi IPR017945. DHBP_synth_RibB-like_a/b_dom.
IPR000422. DHBP_synthase_RibB.
IPR000926. GTP_CycHdrlaseII_RibA.
IPR016299. Riboflavin_synth_RibBA.
[Graphical view ]
Pfami PF00926. DHBP_synthase. 1 hit.
PF00925. GTP_cyclohydro2. 1 hit.
[Graphical view ]
PIRSFi PIRSF001259. RibA. 1 hit.
SUPFAMi SSF55821. SSF55821. 1 hit.
TIGRFAMsi TIGR00505. ribA. 1 hit.
TIGR00506. ribB. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "Complete sequence of Desulfovibrio desulfuricans subsp. desulfuricans str. ATCC 27774."
    US DOE Joint Genome Institute
    Lucas S., Copeland A., Lapidus A., Glavina del Rio T., Tice H., Bruce D., Goodwin L., Pitluck S., Sims D., Lu M., Kiss H., Meineke L., Brettin T., Detter J.C., Han C., Larimer F., Land M., Hauser L.
    , Kyrpides N., Ovchinnikova G., Hazen T.C.
    Submitted (JAN-2009) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 27774 / DSM 6949.

Entry informationi

Entry nameiB8J3Y7_DESDA
AccessioniPrimary (citable) accession number: B8J3Y7
Entry historyi
Integrated into UniProtKB/TrEMBL: March 3, 2009
Last sequence update: March 3, 2009
Last modified: June 11, 2014
This is version 38 of the entry and version 1 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Multifunctional enzymeUniRule annotation

External Data

Dasty 3

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