B8J0C2 (FTHS_DESDA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Formate--tetrahydrofolate ligase
EC=6.3.4.3

Alternative name(s):
Formyltetrahydrofolate synthetase
Short name=FHS
Short name=FTHFS

Gene names

Name:	fhs
Ordered Locus Names:	Ddes_1296

Organism

Desulfovibrio desulfuricans (strain ATCC 27774 / DSM 6949) [Complete proteome] [HAMAP]

Taxonomic identifier

525146 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › delta/epsilon subdivisions › Deltaproteobacteria › Desulfovibrionales › Desulfovibrionaceae › Desulfovibrio › Desulfovibrio desulfuricans › Desulfovibrio desulfuricans subsp. desulfuricans

Protein attributes

Sequence length	591 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + formate + tetrahydrofolate = ADP + phosphate + 10-formyltetrahydrofolate. HAMAP-Rule MF_01543
Pathway	One-carbon metabolism; tetrahydrofolate interconversion. HAMAP-Rule MF_01543
Sequence similarities	Belongs to the formate--tetrahydrofolate ligase family.

Ontologies

Keywords
Biological process	One-carbon metabolism
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	folic acid-containing compound biosynthetic process Inferred from electronic annotation. Source: InterPro tetrahydrofolate interconversion Inferred from electronic annotation. Source: UniProtKB-UniPathway
Molecular_function	ATP binding Inferred from electronic annotation. Source: HAMAP formate-tetrahydrofolate ligase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 591	591	Formate--tetrahydrofolate ligase HAMAP-Rule MF_01543		PRO_1000185254
Regions
	Nucleotide binding	74 – 81	8	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	B8J0C2 [UniParc]. Last modified March 3, 2009. Version 1. Checksum: 5DE7F8F29F1E03F5	FASTA	591	63,964
	10 20 30 40 50 60 MTLDPTKHPD WKIALDAESR MKTVETLAAE LGLEKAELLP YGHYMGKVEQ QAVMQRLKDR 70 80 90 100 110 120 PNGKYIDVTA ITPTPLGEGK STTTIGLVQG LARRGRRSSA AIRQPSGGPT MGMKGSAAGG 130 140 150 160 170 180 GLSQCIPLTP YSLNFTGDLH AVGAAHNLGM TALTSRMQHE RNYDDATLEK LSGMARLNID 190 200 210 220 230 240 PTRINTGWVM DFCVQALRNI IIGIEGDGRR NDGFMMRSHF DITVASEVMC ILSIARDLRD 250 260 270 280 290 300 LRERMGRMVL AHDRNGKPVT TSDLGVAGAM TAWLVEAAKP NLIQTIEGQP VFVHTGPFAN 310 320 330 340 350 360 IALGQSSVIA DRVALKLSDY HVTESGFAAE MGYEKFWNLK CHYSGLVPDA AVVVATVRAL 370 380 390 400 410 420 KNHGGAPQPK PGQALPEPYT REDVGLVEAG CANLLHHLGI VRRSGVPAVV CINKFHTDTK 430 440 450 460 470 480 AEVDAIRRIC EQAGARVALS EHWEKGGEGA LELADAVTDA CNEKNEFRPL YNWNSPLTER 490 500 510 520 530 540 ITTIAQEVYG ADGVEFEPLA AQRLKDLQER PDADELGVCM VKTQYSLSDK PALKGVPKGW 550 560 570 580 590 RLHVRDVLFF GGAGLVAPVS GDISLMPGTG SKPAFRNIDV DVETGKVTGL F

References

[1]

"Complete sequence of Desulfovibrio desulfuricans subsp. desulfuricans str. ATCC 27774." US DOE Joint Genome Institute Lucas S., Copeland A., Lapidus A., Glavina del Rio T., Tice H., Bruce D., Goodwin L., Pitluck S., Sims D., Lu M., Kiss H., Meineke L., Brettin T., Detter J.C., Han C., Larimer F., Land M., Hauser L. , Kyrpides N., Ovchinnikova G., Hazen T.C. Submitted (JAN-2009) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 27774 / DSM 6949.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP001358 Genomic DNA. Translation: ACL49199.1.
RefSeq	YP_002479877.1. NC_011883.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	525146.Ddes_1296.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	ACL49199; ACL49199; Ddes_1296.
GeneID	7284988.
KEGG	dds:Ddes_1296.
PATRIC	21736306. VBIDesDes50650_1507.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG2759.
HOGENOM	HOG000040280.
KO	K01938.
ProtClustDB	PRK13507.
Enzyme and pathway databases
BioCyc	DDES525146:GIWF-1337-MONOMER.
UniPathway	UPA00193.
Family and domain databases
HAMAP	MF_01543. FTHFS.
InterPro	IPR000559. Formate_THF_ligase. IPR020628. Formate_THF_ligase_CS. [Graphical view]
Pfam	PF01268. FTHFS. 1 hit. [Graphical view]
PROSITE	PS00721. FTHFS_1. 1 hit. PS00722. FTHFS_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

FTHS_DESDA

Accession

Primary (citable) accession number: B8J0C2

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 28, 2009
Last sequence update:	March 3, 2009
Last modified:	May 1, 2013
This is version 23 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program