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Protein

Catalase-peroxidase

Gene

katG

Organism
Methylobacterium nodulans (strain LMG 21967 / CNCM I-2342 / ORS 2060)
Status
Unreviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Bifunctional enzyme with both catalase and broad-spectrum peroxidase activity.UniRule annotationSAAS annotation

Catalytic activityi

2 H2O2 = O2 + 2 H2O.UniRule annotationSAAS annotation
Donor + H2O2 = oxidized donor + 2 H2O.UniRule annotationSAAS annotation

Cofactori

heme bUniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei93 – 931Transition state stabilizerUniRule annotation
Active sitei97 – 971Proton acceptorUniRule annotation
Metal bindingi259 – 2591Iron (heme axial ligand)UniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase, Peroxidase

Keywords - Biological processi

Hydrogen peroxideUniRule annotationSAAS annotation

Keywords - Ligandi

HemeUniRule annotationSAAS annotation, Iron, Metal-binding

Enzyme and pathway databases

BioCyciMNOD460265:GCZK-8680-MONOMER.

Protein family/group databases

PeroxiBasei7338. MnoCP01.

Names & Taxonomyi

Protein namesi
Recommended name:
Catalase-peroxidaseUniRule annotation (EC:1.11.1.21UniRule annotation)
Short name:
CPUniRule annotation
Alternative name(s):
Peroxidase/catalaseUniRule annotation
Gene namesi
Name:katGUniRule annotation
Ordered Locus Names:Mnod_8179Imported
Encoded oniPlasmid pMNOD02Imported
OrganismiMethylobacterium nodulans (strain LMG 21967 / CNCM I-2342 / ORS 2060)Imported
Taxonomic identifieri460265 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesMethylobacteriaceaeMethylobacterium
Proteomesi
  • UP000008207 Componenti: Plasmid pMNOD02

PTM / Processingi

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Cross-linki96 ↔ 218Tryptophyl-tyrosyl-methioninium (Trp-Tyr) (with M-244)UniRule annotation
Cross-linki218 ↔ 244Tryptophyl-tyrosyl-methioninium (Tyr-Met) (with W-96)UniRule annotation

Post-translational modificationi

The covalent Trp-Tyr-Met adduct is important for the catalase, but not the peroxidase activity of the enzyme.UniRule annotation

Structurei

3D structure databases

ProteinModelPortaliB8IXB4.
SMRiB8IXB4. Positions 21-727.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini130 – 413284PEROXIDASE_4InterPro annotationAdd
BLAST

Sequence similaritiesi

Belongs to the peroxidase family. Peroxidase/catalase subfamily.UniRule annotationSAAS annotation

Phylogenomic databases

HOGENOMiHOG000218110.
KOiK03782.
OMAiIAEVYAC.
OrthoDBiPOG091H05R1.

Family and domain databases

HAMAPiMF_01961. Catal_peroxid. 1 hit.
InterProiIPR000763. Catalase_peroxidase.
IPR010255. Haem_peroxidase.
IPR002016. Haem_peroxidase_pln/fun/bac.
IPR019794. Peroxidases_AS.
IPR019793. Peroxidases_heam-ligand_BS.
[Graphical view]
PfamiPF00141. peroxidase. 2 hits.
[Graphical view]
PRINTSiPR00460. BPEROXIDASE.
PR00458. PEROXIDASE.
SUPFAMiSSF48113. SSF48113. 2 hits.
TIGRFAMsiTIGR00198. cat_per_HPI. 1 hit.
PROSITEiPS00435. PEROXIDASE_1. 1 hit.
PS00436. PEROXIDASE_2. 1 hit.
PS50873. PEROXIDASE_4. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

B8IXB4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDAKVDDTGE GKCPITGSRR GRTNRDWWPN HLDIQVLHRN SSLSDPMGED
60 70 80 90 100
FDYAKEFKTL DLNAVIKDLH ALMTDSQDWW PADFGHYGGL MIRMAWHSAG
110 120 130 140 150
TYRITDGRGG AGAGQQRFAP LNSWPDNANL DKARRLLWPI KQKYGRKISW
160 170 180 190 200
ADLMILAGNV ALESMGFKTF GFAGGRKDVW EPEELYWGPE GTWLGDERYS
210 220 230 240 250
GERQLQEPLG AVQMGLIYVN PEGPNGKPDP VAAARDIRET FFRMAMNDEE
260 270 280 290 300
TVALIAGGHT FGKTHGAGDP SFMGPEPEAG ALEDQGLGWK SRHGRGFGPD
310 320 330 340 350
TITGGPEVIW SQTPTKWSNY FFDNLFKYEW ELTKSPAGAW QWQAKGAEPT
360 370 380 390 400
IPDPFDESKK RMPTMLITDL ALRLDPAYEK ISRRFYEHPD QFADAFARAW
410 420 430 440 450
FKLTHRDMGP IQRYLGPLVP KEVLIWQDPI PALDHEVVNE QDIAVLKAKI
460 470 480 490 500
LASGPSVPQL VSTAWASAST FRGSDKRGGA NGARIRLAPQ KDWEVNQPAE
510 520 530 540 550
LAKVLQKLEV IQKEFNASQK GGKKVSLADL IVLGGCAAVE KAAKDAETPV
560 570 580 590 600
TVPFTPGRMD ASQEQTDVDS FAPLEPRADG FRNYISGRRQ FMMPEEALVD
610 620 630 640 650
RAQLLRLTAP EMTVLVGGLR VLGANVGQSK HGVFTNRPGM LTNDFFLNLL
660 670 680 690 700
DMGTQWHHAG GDVYEGRDRK TNELRWTGTR VDLIFGSHSQ LRALAEVYGC
710 720 730 740
ADSQEKFVKD FVAAWTKVMN LDRFDVAGRI AEKAADLKVS VDAAL
Length:745
Mass (Da):82,850
Last modified:March 3, 2009 - v1
Checksum:i9AFADF79A2DA029A
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001351 Genomic DNA. Translation: ACL63155.1.
RefSeqiWP_012631354.1. NC_011887.1.

Genome annotation databases

EnsemblBacteriaiACL63155; ACL63155; Mnod_8179.
KEGGimno:Mnod_8179.
PATRICi22541154. VBIMetNod76414_0335.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001351 Genomic DNA. Translation: ACL63155.1.
RefSeqiWP_012631354.1. NC_011887.1.

3D structure databases

ProteinModelPortaliB8IXB4.
SMRiB8IXB4. Positions 21-727.
ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

PeroxiBasei7338. MnoCP01.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiACL63155; ACL63155; Mnod_8179.
KEGGimno:Mnod_8179.
PATRICi22541154. VBIMetNod76414_0335.

Phylogenomic databases

HOGENOMiHOG000218110.
KOiK03782.
OMAiIAEVYAC.
OrthoDBiPOG091H05R1.

Enzyme and pathway databases

BioCyciMNOD460265:GCZK-8680-MONOMER.

Family and domain databases

HAMAPiMF_01961. Catal_peroxid. 1 hit.
InterProiIPR000763. Catalase_peroxidase.
IPR010255. Haem_peroxidase.
IPR002016. Haem_peroxidase_pln/fun/bac.
IPR019794. Peroxidases_AS.
IPR019793. Peroxidases_heam-ligand_BS.
[Graphical view]
PfamiPF00141. peroxidase. 2 hits.
[Graphical view]
PRINTSiPR00460. BPEROXIDASE.
PR00458. PEROXIDASE.
SUPFAMiSSF48113. SSF48113. 2 hits.
TIGRFAMsiTIGR00198. cat_per_HPI. 1 hit.
PROSITEiPS00435. PEROXIDASE_1. 1 hit.
PS00436. PEROXIDASE_2. 1 hit.
PS50873. PEROXIDASE_4. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiB8IXB4_METNO
AccessioniPrimary (citable) accession number: B8IXB4
Entry historyi
Integrated into UniProtKB/TrEMBL: March 3, 2009
Last sequence update: March 3, 2009
Last modified: September 7, 2016
This is version 60 of the entry and version 1 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteome, PlasmidImported, Reference proteomeImported

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.