B8IVT3 (B8IVT3_METNO) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names

Recommended name:
Choline dehydrogenase HAMAP MF_00750
Short name=CDH HAMAP MF_00750
Short name=CHD HAMAP MF_00750
EC=1.1.99.1 HAMAP MF_00750

Gene names

Name:	betA HAMAP MF_00750
Ordered Locus Names:	Mnod_8399

Encoded on

Plasmid pMNOD01 EMBL ACL62523.1

Organism

Methylobacterium nodulans (strain ORS2060 / LMG 21967) [Complete proteome] [HAMAP]

Taxonomic identifier

460265 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Methylobacteriaceae › Methylobacterium

Protein attributes

Sequence length	574 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Can catalyze the oxidation of choline to betaine aldehyde and betaine aldehyde to glycine betaine By similarity. HAMAP MF_00750 SAAS SAAS007867
Catalytic activity	Choline + acceptor = betaine aldehyde + reduced acceptor. HAMAP MF_00750 RuleBase RU003969 SAAS SAAS007867
Cofactor	FAD By similarity. HAMAP MF_00750 SAAS SAAS007867 RuleBase RU003968
Pathway	Amine and polyamine biosynthesis; betaine biosynthesis via choline pathway; betaine aldehyde from choline (cytochrome c reductase route): step 1/1. HAMAP MF_00750 RuleBase RU003969 SAAS SAAS007867
Sequence similarities	Belongs to the GMC oxidoreductase family. HAMAP MF_00750 RuleBase RU003968

Ontologies

Keywords
Ligand	FAD HAMAP MF_00750 SAAS SAAS011533 Flavoprotein HAMAP MF_00750 SAAS SAAS011533 RuleBase RU003968
Molecular function	Oxidoreductase HAMAP MF_00750 SAAS SAAS007867
Technical term	Complete proteome Plasmid EMBL ACL62523.1
Gene Ontology (GO)
Biological process	glycine betaine biosynthetic process from choline Inferred from electronic annotation. Source: HAMAP
Molecular function	choline dehydrogenase activity Inferred from electronic annotation. Source: HAMAP flavin adenine dinucleotide binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Regions
	Nucleotide binding	10 – 39	30	FAD By similarity HAMAP MF_00750
Sites
	Active site	478	1	By similarity HAMAP MF_00750

Sequences

	Sequence		Length	Mass (Da)
	B8IVT3 [UniParc]. Last modified March 3, 2009. Version 1. Checksum: 310371EB1461DD2C	FASTA	574	62,758
	10 20 30 40 50 60 MPQTDAREYD FIIVGAGSAG NVLATRLTED PDTTVLLLEA GGPDYRLDFR TQMPAALAYP 70 80 90 100 110 120 LQGRRYNWAY RTDPEPHMNN RRMECGRGKG LGGSSLINGM CYIRGNALDY DGWAQRPGLA 130 140 150 160 170 180 DWSYHDCLPY FRKAESRDVG PDAYHGGDGP LSVTTAKPGS NVLFEAMVEA GVQAGYPRTP 190 200 210 220 230 240 DLNGHQQEGF GPMDRSVTRH GRRSSTARGY LDQARARPNL TIVTHALTDR ILFEGRRASG 250 260 270 280 290 300 VAYLRGGRGP VVARACREVL LCSGAIASPQ ILQRSGVGPA DLLRGLDIPV VHDLPGVGEN 310 320 330 340 350 360 LQDHLEIYVQ FECREPVSLA PALKLHNQPL IGAQWLFTGT GIGASNQFEA GGFIRSSPDF 370 380 390 400 410 420 AWPNLQYHFL PLAINYNGTN PIKSHGFQAH VGSMRSPSRG RIRLTSRDPN DHPSLLFNYM 430 440 450 460 470 480 SREQDWREFR DAIHITREII AQPALDPYRG REISPGADLR SDAELDAFVR AHAETAYHPS 490 500 510 520 530 540 CSCKMGEDAM AVVDGQGRVH GLEGLRVVDA SIMPLIVTGN LNAPTIMIAE KIADRIRGRA 550 560 570 PLPRSEAPYF VAGDTPTRCH PVRGGGASGR RPPS

References

[1]

"Complete sequence of plasmid 1 of Methylobacterium nodulans ORS 2060." Lucas S., Copeland A., Lapidus A., Glavina del Rio T., Dalin E., Tice H., Bruce D., Goodwin L., Pitluck S., Sims D., Brettin T., Detter J.C., Han C., Larimer F., Land M., Hauser L., Kyrpides N., Ivanova N., Marx C.J., Richardson P. Submitted (JAN-2009) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ORS2060 / LMG 21967.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP001350 Genomic DNA. Translation: ACL62523.1.
RefSeq	YP_002495015.1. NC_011892.1.
3D structure databases
ProteinModelPortal	B8IVT3.
ModBase	Search...
Protein-protein interaction databases
STRING	B8IVT3.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	7300664.
GenomeReviews	Gene locus Mnod_8399 in contig CP001350_GR.
KEGG	mno:Mnod_8399.
PATRIC	22541568. VBIMetNod76414_0540.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG734713.
OMA	EISPGAH.
ProtClustDB	PRK02106.
Family and domain databases
HAMAP	MF_00750. Choline_dehydrogen. [Tree]
InterPro	IPR011533. Choline_dehydrogenase. IPR012132. GMC_OxRdtase. IPR000172. GMC_OxRdtase_N. IPR007867. GMC_OxRtase_C. [Graphical view]
KO	K00108.
Pfam	PF05199. GMC_oxred_C. 1 hit. PF00732. GMC_oxred_N. 1 hit. [Graphical view]
PIRSF	PIRSF000137. Alcohol_oxidase. 1 hit.
TIGRFAMs	TIGR01810. BetA. 1 hit.
PROSITE	PS00623. GMC_OXRED_1. 1 hit. PS00624. GMC_OXRED_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

B8IVT3_METNO

Accession

Primary (citable) accession number: B8IVT3

Entry history

Integrated into UniProtKB/TrEMBL:	March 3, 2009
Last sequence update:	March 3, 2009
Last modified:	December 14, 2011
This is version 25 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)