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B8IU36 (BIOB_METNO) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 38. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Biotin synthase

EC=2.8.1.6
Gene names
Name:bioB
Ordered Locus Names:Mnod_6069
OrganismMethylobacterium nodulans (strain ORS2060 / LMG 21967) [Complete proteome] [HAMAP]
Taxonomic identifier460265 [NCBI]
Taxonomic lineageBacteriaProteobacteriaAlphaproteobacteriaRhizobialesMethylobacteriaceaeMethylobacterium

Protein attributes

Sequence length333 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the conversion of dethiobiotin (DTB) to biotin by the insertion of a sulfur atom into dethiobiotin via a radical-based mechanism By similarity. HAMAP-Rule MF_01694

Catalytic activity

Dethiobiotin + sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine = biotin + (sulfur carrier) + 2 L-methionine + 2 5'-deoxyadenosine. HAMAP-Rule MF_01694

Cofactor

Binds 1 4Fe-4S cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine By similarity.

Binds 1 2Fe-2S cluster. The cluster is coordinated with 3 cysteines and 1 arginine By similarity.

Pathway

Cofactor biosynthesis; biotin biosynthesis; biotin from 7,8-diaminononanoate: step 2/2. HAMAP-Rule MF_01694

Subunit structure

Homodimer By similarity. HAMAP-Rule MF_01694

Sequence similarities

Belongs to the radical SAM superfamily. Biotin synthase family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 333333Biotin synthase HAMAP-Rule MF_01694
PRO_0000381467

Sites

Metal binding621Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
Metal binding661Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
Metal binding691Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
Metal binding1071Iron-sulfur 2 (2Fe-2S) By similarity
Metal binding1391Iron-sulfur 2 (2Fe-2S) By similarity
Metal binding1991Iron-sulfur 2 (2Fe-2S) By similarity
Metal binding2711Iron-sulfur 2 (2Fe-2S) By similarity

Sequences

Sequence LengthMass (Da)Tools
B8IU36 [UniParc].

Last modified March 3, 2009. Version 1.
Checksum: ADD7E0EC2489EDBD

FASTA33335,558
        10         20         30         40         50         60 
MTTTTERPDA PIRHDWTVAE IQAIYDLPLL DLVHRASLVH RAHHDPADIQ RASLLSIKTG 

        70         80         90        100        110        120 
GCPEDCAYCP QSAHHKEAGI GRQRLMPVEA VLREAEAAKA AGATRFCMGA AWRQPKDGPE 

       130        140        150        160        170        180 
FDAVLAMVRG VRGLGMEACV TLGMLTPSQA ERLAEAGLTA YNHNLDTGPD FYGDIISTRT 

       190        200        210        220        230        240 
YADRLNTLQA VRDAGIGVCC GGIIGMGEGV ADRAAMLQVL ANHAPHPESV PINALVAVAG 

       250        260        270        280        290        300 
TPLAERPPVD PLDLVRMCAT ARIVMPKARV RLSAGRRALT REAQVLCFLA GANSIFYGER 

       310        320        330 
LLTTANNEAD ADAQLLRDIG VPVPGIEVLE AAE 

« Hide

References

[1]"Complete sequence of chromosome of Methylobacterium nodulans ORS 2060."
US DOE Joint Genome Institute
Lucas S., Copeland A., Lapidus A., Glavina del Rio T., Dalin E., Tice H., Bruce D., Goodwin L., Pitluck S., Sims D., Brettin T., Detter J.C., Han C., Larimer F., Land M., Hauser L., Kyrpides N., Ivanova N., Marx C.J., Richardson P.
Submitted (JAN-2009) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ORS2060 / LMG 21967.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP001349 Genomic DNA. Translation: ACL60894.1.
RefSeqYP_002501197.1. NC_011894.1.

3D structure databases

ProteinModelPortalB8IU36.
SMRB8IU36. Positions 13-321.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING460265.Mnod_6069.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaACL60894; ACL60894; Mnod_6069.
GeneID7305563.
KEGGmno:Mnod_6069.
PATRIC22553951. VBIMetNod76414_6660.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0502.
HOGENOMHOG000239957.
KOK01012.
OMAADRFCMG.
OrthoDBEOG622PMP.
ProtClustDBCLSK2331944.

Enzyme and pathway databases

BioCycMNOD460265:GCZK-6136-MONOMER.
UniPathwayUPA00078; UER00162.

Family and domain databases

Gene3D3.20.20.70. 1 hit.
HAMAPMF_01694. BioB.
InterProIPR013785. Aldolase_TIM.
IPR010722. BATS_dom.
IPR002684. Biotin_synth/BioAB.
IPR024177. Biotin_synthase.
IPR006638. Elp3/MiaB/NifB.
IPR007197. rSAM.
[Graphical view]
PfamPF06968. BATS. 1 hit.
PF04055. Radical_SAM. 1 hit.
[Graphical view]
PIRSFPIRSF001619. Biotin_synth. 1 hit.
SMARTSM00876. BATS. 1 hit.
SM00729. Elp3. 1 hit.
[Graphical view]
TIGRFAMsTIGR00433. bioB. 1 hit.
ProtoNetSearch...

Entry information

Entry nameBIOB_METNO
AccessionPrimary (citable) accession number: B8IU36
Entry history
Integrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: March 3, 2009
Last modified: February 19, 2014
This is version 38 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways