B8IRX8 (CYSD_METNO) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Sulfate adenylyltransferase subunit 2
EC=2.7.7.4

Alternative name(s):
ATP-sulfurylase small subunit
Sulfate adenylate transferase
Short name=SAT

Gene names

Name:	cysD
Ordered Locus Names:	Mnod_1800

Organism

Methylobacterium nodulans (strain ORS2060 / LMG 21967) [Complete proteome] [HAMAP]

Taxonomic identifier

460265 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Methylobacteriaceae › Methylobacterium

Protein attributes

Sequence length	312 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + sulfate = diphosphate + adenylyl sulfate. HAMAP MF_00064
Pathway	Sulfur metabolism; hydrogen sulfide biosynthesis; sulfite from sulfate: step 1/3. HAMAP MF_00064
Subunit structure	Heterodimer composed of CysD, the smaller subunit, and CysN By similarity.
Sequence similarities	Belongs to the PAPS reductase family. CysD subfamily.

Ontologies

Keywords
Ligand	ATP-binding Nucleotide-binding
Molecular function	Nucleotidyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	sulfate reduction Inferred from electronic annotation. Source: InterPro
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW sulfate adenylyltransferase (ATP) activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 312	312	Sulfate adenylyltransferase subunit 2 HAMAP MF_00064		PRO_1000117945

Sequences

	Sequence		Length	Mass (Da)
	B8IRX8 [UniParc]. Last modified March 3, 2009. Version 1. Checksum: A092CEAC548627FB	FASTA	312	35,546
	10 20 30 40 50 60 MSAALKPLAQ LDTPRLTHLQ RLEAESIHIM RETVAETENP VMLYSIGKDS SVLLHLALKA 70 80 90 100 110 120 FAPGRLPFPL LHVDTTWKFR EMIAFRDARA KELGLDLLVY TNPDGLARGI GPVSHGSEVH 130 140 150 160 170 180 TDVMKTQALR QALDKYKFDA AFGGARRDEE ASRAKERIVS LRTAQHRWDP KRQRAEPWHL 190 200 210 220 230 240 YNLKKKRGES LRVFPLSNWT ELDIWLYIEQ ERIPIVPLYF SAERPVVRRD GQLIMVDDER 250 260 270 280 290 300 LPLEPGETPE LRKVRFRTLG CYPLTGAVES DAATLPEIIG ETLAARTSER QGRVIDKDGA 310 GAMERKKQEG YF

References

[1]

"Complete sequence of chromosome of Methylobacterium nodulans ORS 2060." Lucas S., Copeland A., Lapidus A., Glavina del Rio T., Dalin E., Tice H., Bruce D., Goodwin L., Pitluck S., Sims D., Brettin T., Detter J.C., Han C., Larimer F., Land M., Hauser L., Kyrpides N., Ivanova N., Marx C.J., Richardson P. Submitted (JAN-2009) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ORS2060 / LMG 21967.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP001349 Genomic DNA. Translation: ACL56790.1.
RefSeq	YP_002497093.1. NC_011894.1.
3D structure databases
ProteinModelPortal	B8IRX8.
SMR	B8IRX8. Positions 15-222.
ModBase	Search...
Protein-protein interaction databases
STRING	B8IRX8.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	7302414.
GenomeReviews	Gene locus Mnod_1800 in contig CP001349_GR.
KEGG	mno:Mnod_1800.
PATRIC	22545838. VBIMetNod76414_2654.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG758022.
OMA	RVIDHDA.
ProtClustDB	PRK05253.
Family and domain databases
HAMAP	MF_00064. Sulf_adenylyltr_sub2. [Tree]
InterPro	IPR002500. PAPS_reduct. IPR014729. Rossmann-like_a/b/a_fold. IPR011784. SO4_adenylTrfase_ssu. [Graphical view]
Gene3D	G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 2 hits.
KO	K00957.
Pfam	PF01507. PAPS_reduct. 1 hit. [Graphical view]
PIRSF	PIRSF002936. CysDAde_trans. 1 hit.
TIGRFAMs	TIGR02039. CysD. 1 hit.
ProtoNet	Search...

Entry information

Entry name

CYSD_METNO

Accession

Primary (citable) accession number: B8IRX8

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 28, 2009
Last sequence update:	March 3, 2009
Last modified:	January 25, 2012
This is version 23 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program