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B8IP71 (PUR5_METNO) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 37. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Phosphoribosylformylglycinamidine cyclo-ligase

EC=6.3.3.1
Alternative name(s):
AIR synthase
AIRS
Phosphoribosyl-aminoimidazole synthetase
Gene names
Name:purM
Ordered Locus Names:Mnod_5547
OrganismMethylobacterium nodulans (strain ORS2060 / LMG 21967) [Complete proteome] [HAMAP]
Taxonomic identifier460265 [NCBI]
Taxonomic lineageBacteriaProteobacteriaAlphaproteobacteriaRhizobialesMethylobacteriaceaeMethylobacterium

Protein attributes

Sequence length355 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + 2-(formamido)-N(1)-(5-phospho-D-ribosyl)acetamidine = ADP + phosphate + 5-amino-1-(5-phospho-D-ribosyl)imidazole. HAMAP-Rule MF_00741

Pathway

Purine metabolism; IMP biosynthesis via de novo pathway; 5-amino-1-(5-phospho-D-ribosyl)imidazole from N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide: step 2/2. HAMAP-Rule MF_00741

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00741.

Sequence similarities

Belongs to the AIR synthase family.

Ontologies

Keywords
   Biological processPurine biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionLigase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_process'de novo' IMP biosynthetic process

Inferred from electronic annotation. Source: UniProtKB-UniPathway

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-KW

phosphoribosylformylglycinamidine cyclo-ligase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 355355Phosphoribosylformylglycinamidine cyclo-ligase HAMAP-Rule MF_00741
PRO_1000148286

Sequences

Sequence LengthMass (Da)Tools
B8IP71 [UniParc].

Last modified March 3, 2009. Version 1.
Checksum: 01DF13AEF52EF4AB

FASTA35536,228
        10         20         30         40         50         60 
MTGERGLTYA EAGVDIDAGN AMVEAIKPLV RSTRRPGADA EIGGFGGLFD LKAAGFKDPI 

        70         80         90        100        110        120 
LVAANDGVGT KVKIAIETGR HDTIGIDLVA MCVNDIVVQG AEPLFFLDYY ATGKLVPGVG 

       130        140        150        160        170        180 
ADIVRGIAEG CRQAGCALIG GETAEMPGLY DGSDYDLAGF SVGAAERGAL LPRPGIAPGD 

       190        200        210        220        230        240 
LVLGLPSSGV HSNGFSLVRR IVAASGLGWD APAPFAPGRS LGEALLTPTR IYVRPLLAAL 

       250        260        270        280        290        300 
KATGAGAIKA LAHITGGGFP DNLPRVLPEG VGIALDLDEI AVPPVFGWLA RTGKVAEAEM 

       310        320        330        340        350 
LRTFNCGIGM VVVVAADRIA SVEAALREAG EAPVRLGRIT PRGEAPVTFS GSLAL 

« Hide

References

[1]"Complete sequence of chromosome of Methylobacterium nodulans ORS 2060."
US DOE Joint Genome Institute
Lucas S., Copeland A., Lapidus A., Glavina del Rio T., Dalin E., Tice H., Bruce D., Goodwin L., Pitluck S., Sims D., Brettin T., Detter J.C., Han C., Larimer F., Land M., Hauser L., Kyrpides N., Ivanova N., Marx C.J., Richardson P.
Submitted (JAN-2009) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ORS2060 / LMG 21967.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP001349 Genomic DNA. Translation: ACL60389.1.
RefSeqYP_002500692.1. NC_011894.1.

3D structure databases

ProteinModelPortalB8IP71.
SMRB8IP71. Positions 47-342.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING460265.Mnod_5547.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaACL60389; ACL60389; Mnod_5547.
GeneID7306974.
KEGGmno:Mnod_5547.
PATRIC22552925. VBIMetNod76414_6155.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0150.
HOGENOMHOG000229090.
KOK01933.
OMANPQENDI.
OrthoDBEOG61CM1V.

Enzyme and pathway databases

BioCycMNOD460265:GCZK-5605-MONOMER.
UniPathwayUPA00074; UER00129.

Family and domain databases

Gene3D3.30.1330.10. 1 hit.
3.90.650.10. 1 hit.
HAMAPMF_00741_B. AIRS_B.
InterProIPR010918. AIR_synth_C_dom.
IPR000728. AIR_synth_N_dom.
IPR004733. PurM_cligase.
IPR016188. PurM_N-like.
[Graphical view]
PfamPF00586. AIRS. 1 hit.
PF02769. AIRS_C. 1 hit.
[Graphical view]
SUPFAMSSF55326. SSF55326. 1 hit.
SSF56042. SSF56042. 1 hit.
TIGRFAMsTIGR00878. purM. 1 hit.
ProtoNetSearch...

Entry information

Entry namePUR5_METNO
AccessionPrimary (citable) accession number: B8IP71
Entry history
Integrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: March 3, 2009
Last modified: May 14, 2014
This is version 37 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways