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B8IN60 (B8IN60_METNO) Unreviewed, UniProtKB/TrEMBL

Last modified February 19, 2014. Version 37. Feed History...

Clusters with 100%, 90%, 50% identity | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
D-alanine--D-alanine ligase HAMAP-Rule MF_00047

EC=6.3.2.4 HAMAP-Rule MF_00047
Alternative name(s):
D-Ala-D-Ala ligase HAMAP-Rule MF_00047
D-alanylalanine synthetase HAMAP-Rule MF_00047
Gene names
Name:ddl HAMAP-Rule MF_00047
Ordered Locus Names:Mnod_7439 EMBL ACL62176.1
OrganismMethylobacterium nodulans (strain ORS2060 / LMG 21967) [Complete proteome] [HAMAP] EMBL ACL62176.1
Taxonomic identifier460265 [NCBI]
Taxonomic lineageBacteriaProteobacteriaAlphaproteobacteriaRhizobialesMethylobacteriaceaeMethylobacterium

Protein attributes

Sequence length307 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Cell wall formation By similarity. HAMAP-Rule MF_00047 SAAS SAAS005905

Catalytic activity

ATP + 2 D-alanine = ADP + phosphate + D-alanyl-D-alanine. HAMAP-Rule MF_00047 SAAS SAAS005905

Cofactor

Binds 2 magnesium or manganese ions per subunit By similarity. HAMAP-Rule MF_00047 SAAS SAAS005905

Pathway

Cell wall biogenesis; peptidoglycan biosynthesis. HAMAP-Rule MF_00047 SAAS SAAS005905

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00047 SAAS SAAS005905.

Sequence similarities

Belongs to the D-alanine--D-alanine ligase family. HAMAP-Rule MF_00047

Contains 1 ATP-grasp domain. HAMAP-Rule MF_00047

Contains ATP-grasp domain. SAAS SAAS005905

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Regions

Domain101 – 301201ATP-grasp By similarity HAMAP-Rule MF_00047
Nucleotide binding128 – 18255ATP By similarity HAMAP-Rule MF_00047

Sites

Metal binding2511Magnesium or manganese 1 By similarity HAMAP-Rule MF_00047
Metal binding2681Magnesium or manganese 1 By similarity HAMAP-Rule MF_00047
Metal binding2681Magnesium or manganese 2 By similarity HAMAP-Rule MF_00047
Metal binding2701Magnesium or manganese 2 By similarity HAMAP-Rule MF_00047

Sequences

Sequence LengthMass (Da)Tools
B8IN60 [UniParc].

Last modified March 3, 2009. Version 1.
Checksum: 8F0128A84BAF4E5F

FASTA30733,057
        10         20         30         40         50         60 
MSKHVAVLMG GWSSEREVSL NSGRACARAL EAQGFRITPI DVQRNLAAVL AEVQPDIAFN 

        70         80         90        100        110        120 
ALHGPCGEDG TVQGLLELMA IPYTHSGVLA SALAMHKDRA KVVMRSAGVP VPEGRIVNRF 

       130        140        150        160        170        180 
EAASRHVLPP PYVLKPVAEG SSVGVVIVRE GRSHPPQELA REDWSYGDEV LAETYIPGRE 

       190        200        210        220        230        240 
LTCAVMGDTA LGVIDIRPAT GEWYDYDAKY AKGGSVHVLP AEIKPKIYQR VRELALTAHQ 

       250        260        270        280        290        300 
ALGCRGVSRA DLRYDDTPGG TGALVVLEVN TQPGMTETSL VPELAAHAGY SFGELVRWMV 


EDASLNR 

« Hide

References

[1]"Complete sequence of chromosome of Methylobacterium nodulans ORS 2060."
US DOE Joint Genome Institute
Lucas S., Copeland A., Lapidus A., Glavina del Rio T., Dalin E., Tice H., Bruce D., Goodwin L., Pitluck S., Sims D., Brettin T., Detter J.C., Han C., Larimer F., Land M., Hauser L., Kyrpides N., Ivanova N., Marx C.J., Richardson P.
Submitted (JAN-2009) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ORS2060 / LMG 21967.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP001349 Genomic DNA. Translation: ACL62176.1.
RefSeqYP_002502479.1. NC_011894.1.

3D structure databases

ProteinModelPortalB8IN60.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING460265.Mnod_7439.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaACL62176; ACL62176; Mnod_7439.
GeneID7307817.
KEGGmno:Mnod_7439.
PATRIC22556575. VBIMetNod76414_7951.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG1181.
HOGENOMHOG000011592.
KOK01921.
OMADTQYRIP.
OrthoDBEOG6ND0KB.
ProtClustDBPRK01372.

Enzyme and pathway databases

BioCycMNOD460265:GCZK-7525-MONOMER.
UniPathwayUPA00219.

Family and domain databases

Gene3D3.30.1490.20. 1 hit.
3.30.470.20. 2 hits.
3.40.50.20. 1 hit.
HAMAPMF_00047. Dala_Dala_lig.
InterProIPR011761. ATP-grasp.
IPR013815. ATP_grasp_subdomain_1.
IPR013816. ATP_grasp_subdomain_2.
IPR000291. D-Ala_lig_Van_CS.
IPR005905. D_ala_D_ala.
IPR011095. Dala_Dala_lig_C.
IPR011127. Dala_Dala_lig_N.
IPR016185. PreATP-grasp_dom.
[Graphical view]
PANTHERPTHR23132. PTHR23132. 1 hit.
PfamPF07478. Dala_Dala_lig_C. 1 hit.
PF01820. Dala_Dala_lig_N. 2 hits.
[Graphical view]
SUPFAMSSF52440. SSF52440. 1 hit.
TIGRFAMsTIGR01205. D_ala_D_alaTIGR. 1 hit.
PROSITEPS50975. ATP_GRASP. 1 hit.
PS00843. DALA_DALA_LIGASE_1. 1 hit.
PS00844. DALA_DALA_LIGASE_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameB8IN60_METNO
AccessionPrimary (citable) accession number: B8IN60
Entry history
Integrated into UniProtKB/TrEMBL: March 3, 2009
Last sequence update: March 3, 2009
Last modified: February 19, 2014
This is version 37 of the entry and version 1 of the sequence. [Complete history]
Entry statusUnreviewed (UniProtKB/TrEMBL)