B8IL44 (B8IL44_METNO) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names

Recommended name:
DNA topoisomerase RuleBase RU000518
EC=5.99.1.2 RuleBase RU000518

Gene names

Ordered Locus Names:

Mnod_3309

Organism

Methylobacterium nodulans (strain ORS2060 / LMG 21967) [Complete proteome] [HAMAP]

Taxonomic identifier

460265 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Methylobacteriaceae › Methylobacterium

Protein attributes

Sequence length	888 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Releases the supercoiling and torsional tension of DNA introduced during the DNA replication and transcription by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand. The free DNA strand than undergoes passage around the unbroken strand thus removing DNA supercoils. Finally, in the religation step, the DNA 3'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone By similarity. SAAS SAAS000380
Catalytic activity	ATP-independent breakage of single-stranded DNA, followed by passage and rejoining. RuleBase RU000518 SAAS SAAS005733
Sequence similarities	Belongs to the prokaryotic type I/III topoisomerase family. RuleBase RU004206 Contains 1 Toprim domain. SAAS SAAS023406

Ontologies

Keywords
Ligand	ATP-binding SAAS SAAS005733 DNA-binding SAAS SAAS005733 RuleBase RU000518 Nucleotide-binding
Molecular function	Isomerase Topoisomerase SAAS SAAS005733 RuleBase RU000518
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	DNA topological change Inferred from electronic annotation. Source: InterPro
Cellular component	chromosome Inferred from electronic annotation. Source: InterPro
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW DNA topoisomerase type I activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequences

	Sequence		Length	Mass (Da)
	B8IL44 [UniParc]. Last modified March 3, 2009. Version 1. Checksum: B0A9FB9C4C754576	FASTA	888	96,979
	10 20 30 40 50 60 MKVVVVESPA KAKTINKYLG HGYEVLASFG HVRDLPAKDG SVDPEQDFKM LWELEDRGAK 70 80 90 100 110 120 RVSEIARAVK GADKLILATD PDREGEAISW HVLEALQAKR VLKDVSVERV TFNAITKDAV 130 140 150 160 170 180 QTAMAQPRQI DQALVDAYLA RRALDYLVGF NLSPVLWRKL PGARSAGRVQ SVALRLVCDR 190 200 210 220 230 240 EAEIEAFRPR EFWSLVATLA TARGAVFDAR LVGADGKRIQ RLDIGTAEEA EAFRRDLELA 250 260 270 280 290 300 TFTVGNVEAK PARRHPQPPF TTSTLQQEAS RKLGLAPAQT MRIAQRLYEG VEIGGETVGL 310 320 330 340 350 360 ITYMRTDGVD MAPEAIQDAR RVIGAEFGDA YVPAAPRSYS VKAKNAQEAH EAVRPTDLGR 370 380 390 400 410 420 LPKEVARFLE PEQARLYELI WIRTVASQME SAELERTTVD IAAQVGPRRL DLRATGQVVK 430 440 450 460 470 480 FDGFLTLYQE GKDDEEDEES RRLPSMAAGE ALRRERIAAT QHFTEPPPRY SEASLVKRME 490 500 510 520 530 540 ELGIGRPSTY AAVLQVLRDR EYVRLDKKRL VPEDKGRLVT GFLESFFRRY VEYDFTADLE 550 560 570 580 590 600 EQLDRISNAE IDWRAVLRDF WRDFSAAIAG TKELRTAEVL EALNQLLGPH IFPPRADGAN 610 620 630 640 650 660 PRACPNCASG TLSLKLGKFG AFVGCSNYPE CKYTRQLSAA GVEGEGDGSS GEGGQPGQRV 670 680 690 700 710 720 LGEDPATGLP VTLREGRFGP FVQLGEASAE KGAEKPKRSS LPRGLSPAEV SLETALKLLA 730 740 750 760 770 780 LPREVARHPE TGEPILVNLG RFGPYVQHGK TYANLGKGDD LLEIGANRAI DLIVAKEQGG 790 800 810 820 830 840 GRPAADPGRV LGNDPESGRA VTVKAGRYGP YVTDGEVNAT LPKGADRDAI TLDEALRLIA 850 860 870 880 ARREAGGGTK KKATTRTRKS ETAAKPAGKA TKKAPTKASA AKKKAAAE

References

[1]

"Complete sequence of chromosome of Methylobacterium nodulans ORS 2060." Lucas S., Copeland A., Lapidus A., Glavina del Rio T., Dalin E., Tice H., Bruce D., Goodwin L., Pitluck S., Sims D., Brettin T., Detter J.C., Han C., Larimer F., Land M., Hauser L., Kyrpides N., Ivanova N., Marx C.J., Richardson P. Submitted (JAN-2009) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ORS2060 / LMG 21967.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP001349 Genomic DNA. Translation: ACL58232.1.
RefSeq	YP_002498535.1. NC_011894.1.
3D structure databases
ProteinModelPortal	B8IL44.
ModBase	Search...
Protein-protein interaction databases
STRING	B8IL44.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	7301734.
GenomeReviews	Gene locus Mnod_3309 in contig CP001349_GR.
KEGG	mno:Mnod_3309.
PATRIC	22548621. VBIMetNod76414_4034.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG512935.
OMA	KKVMPRL.
ProtClustDB	PRK07561.
Family and domain databases
InterPro	IPR000380. Topo_IA. IPR003601. Topo_IA_2. IPR023406. Topo_IA_AS. IPR013497. Topo_IA_cen. IPR013824. Topo_IA_cen_sub1. IPR013825. Topo_IA_cen_sub2. IPR023405. Topo_IA_core_domain. IPR003602. Topo_IA_DNA-bd. IPR013498. Topo_IA_Znf. IPR005733. TopoI_bac-type. IPR006171. Toprim_domain. [Graphical view]
Gene3D	G3DSA:1.10.460.10. Topo_IA_cen_sub1. 2 hits. G3DSA:2.70.20.10. Topo_IA_cen_sub2. 2 hits.
KO	K03168.
PANTHER	PTHR11390. Topo_IA. 1 hit.
Pfam	PF01131. Topoisom_bac. 1 hit. PF01751. Toprim. 1 hit. PF01396. zf-C4_Topoisom. 1 hit. [Graphical view]
PRINTS	PR00417. PRTPISMRASEI.
SMART	SM00437. TOP1Ac. 1 hit. SM00436. TOP1Bc. 1 hit. SM00493. TOPRIM. 1 hit. [Graphical view]
SUPFAM	SSF56712. Topo_IA_core. 1 hit.
TIGRFAMs	TIGR01051. TopA_bact. 1 hit.
PROSITE	PS00396. TOPOISOMERASE_I_PROK. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

B8IL44_METNO

Accession

Primary (citable) accession number: B8IL44

Entry history

Integrated into UniProtKB/TrEMBL:	March 3, 2009
Last sequence update:	March 3, 2009
Last modified:	December 14, 2011
This is version 26 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)