Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

B8IKV1 (B8IKV1_METNO) Unreviewed, UniProtKB/TrEMBL

Last modified February 19, 2014. Version 36. Feed History...

Clusters with 100%, 90%, 50% identity | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry infoCustomize order

Names and origin

Protein namesRecommended name:
D-alanine--D-alanine ligase HAMAP-Rule MF_00047

EC=6.3.2.4 HAMAP-Rule MF_00047
Alternative name(s):
D-Ala-D-Ala ligase HAMAP-Rule MF_00047
D-alanylalanine synthetase HAMAP-Rule MF_00047
Gene names
Name:ddl HAMAP-Rule MF_00047
Ordered Locus Names:Mnod_1308 EMBL ACL56308.1
OrganismMethylobacterium nodulans (strain ORS2060 / LMG 21967) [Complete proteome] [HAMAP] EMBL ACL56308.1
Taxonomic identifier460265 [NCBI]
Taxonomic lineageBacteriaProteobacteriaAlphaproteobacteriaRhizobialesMethylobacteriaceaeMethylobacterium

Protein attributes

Sequence length378 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Cell wall formation By similarity. HAMAP-Rule MF_00047 SAAS SAAS005905

Catalytic activity

ATP + 2 D-alanine = ADP + phosphate + D-alanyl-D-alanine. HAMAP-Rule MF_00047 SAAS SAAS005905

Cofactor

Binds 2 magnesium or manganese ions per subunit By similarity. HAMAP-Rule MF_00047 SAAS SAAS005905

Pathway

Cell wall biogenesis; peptidoglycan biosynthesis. HAMAP-Rule MF_00047 SAAS SAAS005905

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00047 SAAS SAAS005905.

Sequence similarities

Belongs to the D-alanine--D-alanine ligase family. HAMAP-Rule MF_00047

Contains 1 ATP-grasp domain. HAMAP-Rule MF_00047

Contains ATP-grasp domain. SAAS SAAS005905

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Regions

Domain139 – 349211ATP-grasp By similarity HAMAP-Rule MF_00047
Nucleotide binding169 – 22456ATP By similarity HAMAP-Rule MF_00047

Sites

Metal binding3011Magnesium or manganese 1 By similarity HAMAP-Rule MF_00047
Metal binding3161Magnesium or manganese 1 By similarity HAMAP-Rule MF_00047
Metal binding3161Magnesium or manganese 2 By similarity HAMAP-Rule MF_00047
Metal binding3181Magnesium or manganese 2 By similarity HAMAP-Rule MF_00047

Sequences

Sequence LengthMass (Da)Tools
B8IKV1 [UniParc].

Last modified March 3, 2009. Version 1.
Checksum: EA63ADC57D670C59

FASTA37838,945
        10         20         30         40         50         60 
MRRKVALLFG GRSAEHEVSI LSAGNVARAL DPDRYAVTPI AIDKETGAWR LCPALAAGEA 

        70         80         90        100        110        120 
PAMGQDGPLV AFLPGGGGRL AILSDAAALS EPFDVVVPVL HGPNGEDGTV QGALDLAGVP 

       130        140        150        160        170        180 
YVGSGVVGSA AAMDKDVAKR LMRDAGLPMI VPFLAATARR PVSYADAAAA LESRTLFVKP 

       190        200        210        220        230        240 
ANMGSSVGVS RVTDPAQFDR AAAHAFGYDE KILIERAVPQ AREIECAVLE TPEGELRVSP 

       250        260        270        280        290        300 
PGEIAPAAAH GFYGYDAKYL DPDGAALLIP AALDAARADR MRELAARAFA ALACAGLARV 

       310        320        330        340        350        360 
DLFLDPADPA GIFVNEVNTL PGFTAISMYP KLWDAAGLAP PALMDALIAH ALARHARAVA 

       370 
TGRAAPRPGP DAAGAPMR 

« Hide

References

[1]"Complete sequence of chromosome of Methylobacterium nodulans ORS 2060."
US DOE Joint Genome Institute
Lucas S., Copeland A., Lapidus A., Glavina del Rio T., Dalin E., Tice H., Bruce D., Goodwin L., Pitluck S., Sims D., Brettin T., Detter J.C., Han C., Larimer F., Land M., Hauser L., Kyrpides N., Ivanova N., Marx C.J., Richardson P.
Submitted (JAN-2009) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ORS2060 / LMG 21967.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP001349 Genomic DNA. Translation: ACL56308.1.
RefSeqYP_002496611.1. NC_011894.1.

3D structure databases

ProteinModelPortalB8IKV1.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING460265.Mnod_1308.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaACL56308; ACL56308; Mnod_1308.
GeneID7308060.
KEGGmno:Mnod_1308.
PATRIC22544850. VBIMetNod76414_2163.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG1181.
HOGENOMHOG000011593.
KOK01921.
OMAMDKIAMK.
OrthoDBEOG64BQ73.
ProtClustDBPRK01966.

Enzyme and pathway databases

BioCycMNOD460265:GCZK-1319-MONOMER.
UniPathwayUPA00219.

Family and domain databases

Gene3D3.30.1490.20. 1 hit.
3.30.470.20. 1 hit.
3.40.50.20. 1 hit.
HAMAPMF_00047. Dala_Dala_lig.
InterProIPR011761. ATP-grasp.
IPR013815. ATP_grasp_subdomain_1.
IPR013816. ATP_grasp_subdomain_2.
IPR000291. D-Ala_lig_Van_CS.
IPR005905. D_ala_D_ala.
IPR011095. Dala_Dala_lig_C.
IPR011127. Dala_Dala_lig_N.
IPR016185. PreATP-grasp_dom.
[Graphical view]
PANTHERPTHR23132. PTHR23132. 1 hit.
PfamPF07478. Dala_Dala_lig_C. 1 hit.
PF01820. Dala_Dala_lig_N. 1 hit.
[Graphical view]
SUPFAMSSF52440. SSF52440. 1 hit.
TIGRFAMsTIGR01205. D_ala_D_alaTIGR. 1 hit.
PROSITEPS50975. ATP_GRASP. 1 hit.
PS00843. DALA_DALA_LIGASE_1. 1 hit.
PS00844. DALA_DALA_LIGASE_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameB8IKV1_METNO
AccessionPrimary (citable) accession number: B8IKV1
Entry history
Integrated into UniProtKB/TrEMBL: March 3, 2009
Last sequence update: March 3, 2009
Last modified: February 19, 2014
This is version 36 of the entry and version 1 of the sequence. [Complete history]
Entry statusUnreviewed (UniProtKB/TrEMBL)