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Protein

Malate dehydrogenase

Gene

mdh

Organism
Methylobacterium nodulans (strain LMG 21967 / CNCM I-2342 / ORS 2060)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the reversible oxidation of malate to oxaloacetate.UniRule annotation

Catalytic activityi

(S)-malate + NAD+ = oxaloacetate + NADH.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei34NADUniRule annotation1
Binding sitei83SubstrateUniRule annotation1
Binding sitei89SubstrateUniRule annotation1
Binding sitei96NADUniRule annotation1
Binding sitei121SubstrateUniRule annotation1
Binding sitei152SubstrateUniRule annotation1
Active sitei176Proton acceptorUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi10 – 15NADUniRule annotation6
Nucleotide bindingi119 – 121NADUniRule annotation3

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Tricarboxylic acid cycle

Keywords - Ligandi

NAD

Names & Taxonomyi

Protein namesi
Recommended name:
Malate dehydrogenaseUniRule annotation (EC:1.1.1.37UniRule annotation)
Gene namesi
Name:mdhUniRule annotation
Ordered Locus Names:Mnod_1123
OrganismiMethylobacterium nodulans (strain LMG 21967 / CNCM I-2342 / ORS 2060)
Taxonomic identifieri460265 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesMethylobacteriaceaeMethylobacterium
Proteomesi
  • UP000008207 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_10001916501 – 320Malate dehydrogenaseAdd BLAST320

Interactioni

Protein-protein interaction databases

STRINGi460265.Mnod_1123.

Structurei

3D structure databases

ProteinModelPortaliB8IJB4.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the LDH/MDH superfamily. MDH type 3 family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105C80. Bacteria.
COG0039. LUCA.
HOGENOMiHOG000213794.
KOiK00024.
OMAiMDLMQTA.
OrthoDBiPOG091H02N6.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
3.90.110.10. 1 hit.
HAMAPiMF_00487. Malate_dehydrog_3. 1 hit.
InterProiIPR001557. L-lactate/malate_DH.
IPR022383. Lactate/malate_DH_C.
IPR001236. Lactate/malate_DH_N.
IPR015955. Lactate_DH/Glyco_Ohase_4_C.
IPR011275. Malate_DH_type3.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PANTHERiPTHR11540. PTHR11540. 1 hit.
PfamiPF02866. Ldh_1_C. 1 hit.
PF00056. Ldh_1_N. 1 hit.
[Graphical view]
PIRSFiPIRSF000102. Lac_mal_DH. 1 hit.
PRINTSiPR00086. LLDHDRGNASE.
SUPFAMiSSF51735. SSF51735. 1 hit.
SSF56327. SSF56327. 1 hit.
TIGRFAMsiTIGR01763. MalateDH_bact. 1 hit.

Sequencei

Sequence statusi: Complete.

B8IJB4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MARKKIALIG AGQIGGTLAH LAGLKELGDV VLFDIADGVP QGKGLDIAES
60 70 80 90 100
APVDGFDAKY SGASDYSAIA GADVVIVTAG VPRKPGMSRD DLIGINLKVM
110 120 130 140 150
EAVGTGIKTH APNAFVICIT NPLDAMVWAL QKFSGLDPKK IVGMAGVLDS
160 170 180 190 200
ARFRHFLAEE FSVSVEDVTA FVLGGHGDDM VPLVRYSTVA GIPLPDLVKM
210 220 230 240 250
GWTTQEKLDA MVERTRKGGG EIVNLLKTGS AFYAPAASAI AMAESYLKDK
260 270 280 290 300
KRVLPCAAYL TGQYGVDGLF IGVPIVIGEN GVERIVEVAF SAEEKAMFDK
310 320
SVNSVKGLVE ACKGINAALA
Length:320
Mass (Da):33,469
Last modified:March 3, 2009 - v1
Checksum:i9076DE9ACD30527A
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001349 Genomic DNA. Translation: ACL56129.1.
RefSeqiWP_015927827.1. NC_011894.1.

Genome annotation databases

EnsemblBacteriaiACL56129; ACL56129; Mnod_1123.
KEGGimno:Mnod_1123.
PATRICi22544462. VBIMetNod76414_1970.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001349 Genomic DNA. Translation: ACL56129.1.
RefSeqiWP_015927827.1. NC_011894.1.

3D structure databases

ProteinModelPortaliB8IJB4.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi460265.Mnod_1123.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiACL56129; ACL56129; Mnod_1123.
KEGGimno:Mnod_1123.
PATRICi22544462. VBIMetNod76414_1970.

Phylogenomic databases

eggNOGiENOG4105C80. Bacteria.
COG0039. LUCA.
HOGENOMiHOG000213794.
KOiK00024.
OMAiMDLMQTA.
OrthoDBiPOG091H02N6.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
3.90.110.10. 1 hit.
HAMAPiMF_00487. Malate_dehydrog_3. 1 hit.
InterProiIPR001557. L-lactate/malate_DH.
IPR022383. Lactate/malate_DH_C.
IPR001236. Lactate/malate_DH_N.
IPR015955. Lactate_DH/Glyco_Ohase_4_C.
IPR011275. Malate_DH_type3.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PANTHERiPTHR11540. PTHR11540. 1 hit.
PfamiPF02866. Ldh_1_C. 1 hit.
PF00056. Ldh_1_N. 1 hit.
[Graphical view]
PIRSFiPIRSF000102. Lac_mal_DH. 1 hit.
PRINTSiPR00086. LLDHDRGNASE.
SUPFAMiSSF51735. SSF51735. 1 hit.
SSF56327. SSF56327. 1 hit.
TIGRFAMsiTIGR01763. MalateDH_bact. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiMDH_METNO
AccessioniPrimary (citable) accession number: B8IJB4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: March 3, 2009
Last modified: November 2, 2016
This is version 51 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.