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B8IJ20 (B8IJ20_METNO) Unreviewed, UniProtKB/TrEMBL

Last modified December 14, 2011. Version 24. Feed History...

Clusters with 100%, 90%, 50% identity | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
3-oxoacyl-[acyl-carrier-protein] synthase 3 HAMAP MF_01815
EC=2.3.1.180 HAMAP MF_01815
Alternative name(s):
3-oxoacyl-[acyl-carrier-protein] synthase III HAMAP MF_01815
Beta-ketoacyl-ACP synthase III HAMAP MF_01815
Gene names
Name:fabH HAMAP MF_01815
Ordered Locus Names:Mnod_7075
OrganismMethylobacterium nodulans (strain ORS2060 / LMG 21967) [Complete proteome] [HAMAP]
Taxonomic identifier460265 [NCBI]
Taxonomic lineageBacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Methylobacteriaceae › Methylobacterium

Protein attributes

Sequence length325 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the condensation reaction of fatty acid synthesis by the addition to an acyl acceptor of two carbons from malonyl-ACP. Catalyzes the first condensation reaction which initiates fatty acid synthesis and may therefore play a role in governing the total rate of fatty acid production. Possesses both acetoacetyl-ACP synthase and acetyl transacylase activities. Its substrate specificity determines the biosynthesis of branched-chain and/or straight-chain of fatty acids By similarity. HAMAP MF_01815

Catalytic activity

Acetyl-CoA + malonyl-[acyl-carrier-protein] = acetoacetyl-[acyl-carrier-protein] + CoA + CO2. HAMAP MF_01815

Pathway

Lipid metabolism; fatty acid biosynthesis. HAMAP MF_01815 SAAS SAAS013747

Subunit structure

Homodimer By similarity. HAMAP MF_01815 SAAS SAAS013747

Subcellular location

Cytoplasm By similarity HAMAP MF_01815 SAAS SAAS013747.

Domain

The last Arg residue of the ACP-binding site is essential for the weak association between ACP/AcpP and FabH By similarity. HAMAP MF_01815

Sequence similarities

Belongs to the FabH family. HAMAP MF_01815

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Regions

Region253 – 2575ACP-binding By similarity HAMAP MF_01815

Sites

Active site1151 By similarity HAMAP MF_01815
Active site2521 By similarity HAMAP MF_01815
Active site2821 By similarity HAMAP MF_01815

Sequences

Sequence LengthMass (Da)Tools
B8IJ20 [UniParc].

Last modified March 3, 2009. Version 1.
Checksum: 1247873EBF84CAD7

FASTA32534,194
        10         20         30         40         50         60 
MTRLRSVVRG CGSYLPRTVV TNDDLAARVD TSDEWIVQRT GIRQRHIAGP DETTSVLGIK 

        70         80         90        100        110        120 
AAAAALAAAN LDPASIDLII CATSTPDHTF PSTATQIQAG LGIHGGAAFD LQAVCAGFVY 

       130        140        150        160        170        180 
GVATADKFLT TGAARRALVV GAETFSRLLD WEDRTTCVLF GDGAGAIVLE AQGGEGHSGD 

       190        200        210        220        230        240 
RGVLTSHLRS DGRYREKLYV DGGPGSTGTT GKLRMVGKEV FRFAVGSVTD VIAEAFAATG 

       250        260        270        280        290        300 
TTAEDLDWFV PHQANRRIIE ASADKLGIAR EKVVLTVDRH GNTSAASIPL ALDAACRDGR 

       310        320 
IRRGDLVMIE AIGGGFAWGS ALIRW

« Hide

References

[1]"Complete sequence of chromosome of Methylobacterium nodulans ORS 2060."
Lucas S., Copeland A., Lapidus A., Glavina del Rio T., Dalin E., Tice H., Bruce D., Goodwin L., Pitluck S., Sims D., Brettin T., Detter J.C., Han C., Larimer F., Land M., Hauser L., Kyrpides N., Ivanova N., Marx C.J., Richardson P.
Submitted (JAN-2009) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ORS2060 / LMG 21967.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		CP001349 Genomic DNA. Translation: ACL61815.1.
RefSeqYP_002502118.1. NC_011894.1.

3D structure databases

ProteinModelPortalB8IJ20.
SMRB8IJ20. Positions 6-325.
ModBaseSearch...

Protein-protein interaction databases

STRINGB8IJ20.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID7302973.
GenomeReviewsGene locus Mnod_7075 in contig CP001349_GR.
KEGGmno:Mnod_7075.
PATRIC22555823. VBIMetNod76414_7580.

Organism-specific databases

CMRSearch...

Phylogenomic databases

HOGENOMHBG649927.
OMADAYIRGG.
ProtClustDBCLSK2330945.

Family and domain databases

HAMAPMF_01815. FabH.
[Tree]
InterProIPR013751. ACP_syn_III.
IPR013747. ACP_syn_III_C.
IPR004655. FabH_synth.
IPR016039. Thiolase-like.
IPR016038. Thiolase-like_subgr.
[Graphical view]
Gene3DG3DSA:3.40.47.10. Thiolase-like_subgr. 2 hits.
KOK00648.
PfamPF08545. ACP_syn_III. 1 hit.
PF08541. ACP_syn_III_C. 1 hit.
[Graphical view]
SUPFAMSSF53901. Thiolase-like. 1 hit.
TIGRFAMsTIGR00747. FabH. 1 hit.
ProtoNetSearch...

Entry information

Entry nameB8IJ20_METNO
AccessionPrimary (citable) accession number: B8IJ20
Entry history
Integrated into UniProtKB/TrEMBL: March 3, 2009
Last sequence update: March 3, 2009
Last modified: December 14, 2011
This is version 24 of the entry and version 1 of the sequence. [Complete history]
Entry statusUnreviewed (UniProtKB/TrEMBL)
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