B8IHZ7 (RSMG_METNO) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ribosomal RNA small subunit methyltransferase G
EC=2.1.1.170

Alternative name(s):
16S rRNA 7-methylguanosine methyltransferase
Short name=16S rRNA m7G methyltransferase

Gene names

Name:	rsmG
Ordered Locus Names:	Mnod_1027

Organism

Methylobacterium nodulans (strain ORS2060 / LMG 21967) [Complete proteome] [HAMAP]

Taxonomic identifier

460265 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Methylobacteriaceae › Methylobacterium › Methylobacterium nodulans

Protein attributes

Sequence length	212 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Specifically methylates the N7 position of guanine in position 527 of 16S rRNA By similarity. HAMAP-Rule MF_00074
Catalytic activity	S-adenosyl-L-methionine + guanine(527) in 16S rRNA = S-adenosyl-L-homocysteine + N(7)-methylguanine(527) in 16S rRNA. HAMAP-Rule MF_00074
Subcellular location	Cytoplasm Potential HAMAP-Rule MF_00074.
Sequence similarities	Belongs to the methyltransferase superfamily. RNA methyltransferase RsmG family.

Ontologies

Keywords
Biological process	rRNA processing
Cellular component	Cytoplasm
Ligand	S-adenosyl-L-methionine
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	rRNA (guanine-N7)-methylation Inferred from electronic annotation. Source: GOC
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	rRNA (guanine-N7-)-methyltransferase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 212	212	Ribosomal RNA small subunit methyltransferase G HAMAP-Rule MF_00074		PRO_1000118194
Regions
	Region	127 – 128	2	S-adenosyl-L-methionine binding By similarity
Sites
	Binding site	73	1	S-adenosyl-L-methionine; via carbonyl oxygen By similarity
	Binding site	143	1	S-adenosyl-L-methionine By similarity

Sequences

	Sequence		Length	Mass (Da)
	B8IHZ7 [UniParc]. Last modified March 3, 2009. Version 1. Checksum: ECCE936084395FFF	FASTA	212	22,486
	10 20 30 40 50 60 MSASDRDRVL AAAAVSRETA AALDLYVAQL ARWQTVKNLV GPSTLPEVWT RHIADSLQLL 70 80 90 100 110 120 DAAPGATRWL DLGSGAGIPG LILAIAGREA RPQMQVDLVE SNARKGAFLQ ETARLTGASA 130 140 150 160 170 180 RIHVSRIESV IGRFTGVEVV CARALAPLPQ LLAWTAPLLK SGTIGLFPKG REAQSELTAA 190 200 210 RETWTFEADV IPSRTDSSAG IVRISSLSGQ LP

References

[1]

"Complete sequence of chromosome of Methylobacterium nodulans ORS 2060." US DOE Joint Genome Institute Lucas S., Copeland A., Lapidus A., Glavina del Rio T., Dalin E., Tice H., Bruce D., Goodwin L., Pitluck S., Sims D., Brettin T., Detter J.C., Han C., Larimer F., Land M., Hauser L., Kyrpides N., Ivanova N., Marx C.J., Richardson P. Submitted (JAN-2009) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ORS2060 / LMG 21967.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP001349 Genomic DNA. Translation: ACL56035.1.
RefSeq	YP_002496338.1. NC_011894.1.
3D structure databases
ProteinModelPortal	B8IHZ7.
ModBase	Search...
Protein-protein interaction databases
STRING	460265.Mnod_1027.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	ACL56035; ACL56035; Mnod_1027.
GeneID	7302571.
KEGG	mno:Mnod_1027.
PATRIC	22544258. VBIMetNod76414_1868.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0357.
HOGENOM	HOG000221015.
KO	K03501.
OMA	FHKGRDY.
ProtClustDB	CLSK2330754.
Enzyme and pathway databases
BioCyc	MNOD460265:GCZK-1038-MONOMER.
Family and domain databases
HAMAP	MF_00074. 16SrRNA_methyltr_G.
InterPro	IPR003682. rRNA_ssu_MeTfrase_G. [Graphical view]
Pfam	PF02527. GidB. 1 hit. [Graphical view]
PIRSF	PIRSF003078. GidB. 1 hit.
TIGRFAMs	TIGR00138. gidB. 1 hit.
ProtoNet	Search...

Entry information

Entry name

RSMG_METNO

Accession

Primary (citable) accession number: B8IHZ7

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 28, 2009
Last sequence update:	March 3, 2009
Last modified:	May 29, 2013
This is version 29 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program