B8IHY4 (B8IHY4_METNO) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names

Recommended name:
Pantothenate synthetase HAMAP MF_00158
Short name=PS HAMAP MF_00158
EC=6.3.2.1 HAMAP MF_00158

Alternative name(s):
Pantoate--beta-alanine ligase HAMAP MF_00158
Pantoate-activating enzyme HAMAP MF_00158

Gene names

Name:	panC HAMAP MF_00158
Ordered Locus Names:	Mnod_1014

Organism

Methylobacterium nodulans (strain ORS2060 / LMG 21967) [Complete proteome] [HAMAP]

Taxonomic identifier

460265 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Methylobacteriaceae › Methylobacterium

Protein attributes

Sequence length	290 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the condensation of pantoate with beta-alanine in an ATP-dependent reaction via a pantoyl-adenylate intermediate By similarity. HAMAP MF_00158
Catalytic activity	ATP + (R)-pantoate + beta-alanine = AMP + diphosphate + (R)-pantothenate. HAMAP MF_00158
Pathway	Cofactor biosynthesis; (R)-pantothenate biosynthesis; (R)-pantothenate from (R)-pantoate and beta-alanine: step 1/1. HAMAP MF_00158
Subunit structure	Homodimer By similarity. HAMAP MF_00158
Subcellular location	Cytoplasm By similarity HAMAP MF_00158.
Miscellaneous	The reaction proceeds by a bi uni uni bi ping pong mechanism By similarity. HAMAP MF_00158
Sequence similarities	Belongs to the pantothenate synthetase family. HAMAP MF_00158

Ontologies

Keywords
Biological process	Pantothenate biosynthesis HAMAP MF_00158
Cellular component	Cytoplasm HAMAP MF_00158
Ligand	ATP-binding HAMAP MF_00158 Nucleotide-binding
Molecular function	Ligase HAMAP MF_00158 EMBL ACL56022.1
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	pantothenate biosynthetic process Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW pantoate-beta-alanine ligase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Regions
	Nucleotide binding	42 – 49	8	ATP By similarity HAMAP MF_00158
	Nucleotide binding	159 – 162	4	ATP By similarity HAMAP MF_00158
	Nucleotide binding	196 – 199	4	ATP By similarity HAMAP MF_00158
Sites
	Active site	49	1	Proton donor By similarity HAMAP MF_00158
	Binding site	73	1	Beta-alanine By similarity HAMAP MF_00158
	Binding site	73	1	Pantoate By similarity HAMAP MF_00158
	Binding site	165	1	Pantoate By similarity HAMAP MF_00158
	Binding site	188	1	ATP; via amide nitrogen and carbonyl oxygen By similarity HAMAP MF_00158

Sequences

	Sequence		Length	Mass (Da)
	B8IHY4 [UniParc]. Last modified March 3, 2009. Version 1. Checksum: 597B99F86CCAB535	FASTA	290	30,725
	10 20 30 40 50 60 MTALSPSPAA ETVARLGDVP ALRAQVRAWR AAGESVALVP TMGALHQGHL TLVRRARETC 70 80 90 100 110 120 RRVVVSIFVN PTQFGPNEDF ARYPRDPERD IALLGEAGAD AAYLPDVGTM YPPGFATTVS 130 140 150 160 170 180 VGGLTEVLCG PLRPGHFAGV ATVVTKLLLQ ALPDRALFGE KDYQQLQLIR RFVHDLDIPV 190 200 210 220 230 240 AIEGVPTVRE ADGLALSSRN QYLSAAERAV APRLNAVLRR VAEAVRGGAE TAPALAEGRA 250 260 270 280 290 TLEAAGFGPV QYLSVNDAES LAPLDRVAGP ARVLAAAYLG RTRLIDNVAV

References

[1]

"Complete sequence of chromosome of Methylobacterium nodulans ORS 2060." Lucas S., Copeland A., Lapidus A., Glavina del Rio T., Dalin E., Tice H., Bruce D., Goodwin L., Pitluck S., Sims D., Brettin T., Detter J.C., Han C., Larimer F., Land M., Hauser L., Kyrpides N., Ivanova N., Marx C.J., Richardson P. Submitted (JAN-2009) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ORS2060 / LMG 21967.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP001349 Genomic DNA. Translation: ACL56022.1.
RefSeq	YP_002496325.1. NC_011894.1.
3D structure databases
ProteinModelPortal	B8IHY4.
SMR	B8IHY4. Positions 24-290.
ModBase	Search...
Protein-protein interaction databases
STRING	B8IHY4.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	7302558.
GenomeReviews	Gene locus Mnod_1014 in contig CP001349_GR.
KEGG	mno:Mnod_1014.
PATRIC	22544232. VBIMetNod76414_1855.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG428839.
OMA	EMDGLAM.
ProtClustDB	PRK00380.
Family and domain databases
HAMAP	MF_00158. PanC. [Tree]
InterPro	IPR004821. Cyt_trans-rel. IPR003721. Pantoate_ligase. IPR014729. Rossmann-like_a/b/a_fold. [Graphical view]
Gene3D	G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 1 hit.
KO	K01918.
PANTHER	PTHR21299:SF1. Pantoate_ligase. 1 hit.
Pfam	PF02569. Pantoate_ligase. 1 hit. [Graphical view]
TIGRFAMs	TIGR00125. Cyt_tran_rel. 1 hit. TIGR00018. PanC. 1 hit.
ProtoNet	Search...

Entry information

Entry name

B8IHY4_METNO

Accession

Primary (citable) accession number: B8IHY4

Entry history

Integrated into UniProtKB/TrEMBL:	March 3, 2009
Last sequence update:	March 3, 2009
Last modified:	December 14, 2011
This is version 21 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)