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Protein

Glutamine-dependent NAD(+) synthetase

Gene

Mnod_2890

Organism
Methylobacterium nodulans (strain LMG 21967 / CNCM I-2342 / ORS 2060)
Status
Unreviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

ATP + deamido-NAD+ + L-glutamine + H2O = AMP + diphosphate + NAD+ + L-glutamate.UniRule annotation

Pathwayi: NAD(+) biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes NAD(+) from deamido-NAD(+) (L-Gln route).UniRule annotation
Proteins known to be involved in this subpathway in this organism are:
  1. Glutamine-dependent NAD(+) synthetase (Mnod_2890)
This subpathway is part of the pathway NAD(+) biosynthesis, which is itself part of Cofactor biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes NAD(+) from deamido-NAD(+) (L-Gln route), the pathway NAD(+) biosynthesis and in Cofactor biosynthesis.

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionLigaseUniRule annotationSAAS annotation
LigandATP-bindingUniRule annotationSAAS annotation, NADUniRule annotationSAAS annotation, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00253; UER00334.

Names & Taxonomyi

Protein namesi
Recommended name:
Glutamine-dependent NAD(+) synthetaseUniRule annotation (EC:6.3.5.1UniRule annotation)
Alternative name(s):
NAD(+) synthase [glutamine-hydrolyzing]UniRule annotation
Gene namesi
Ordered Locus Names:Mnod_2890Imported
OrganismiMethylobacterium nodulans (strain LMG 21967 / CNCM I-2342 / ORS 2060)Imported
Taxonomic identifieri460265 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesMethylobacteriaceaeMethylobacterium
Proteomesi
  • UP000008207 Componenti: Chromosome

Interactioni

Protein-protein interaction databases

STRINGi460265.Mnod_2890.

Structurei

3D structure databases

ProteinModelPortaliB8IGW6.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini11 – 299CN hydrolaseInterPro annotationAdd BLAST289

Sequence similaritiesi

In the C-terminal section; belongs to the NAD synthetase family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4108E9H. Bacteria.
COG0171. LUCA.
COG0388. LUCA.
HOGENOMiHOG000051304.
KOiK01950.
OMAiRLAQDCY.
OrthoDBiPOG091H026O.

Family and domain databases

CDDicd00553. NAD_synthase. 1 hit.
Gene3Di3.40.50.620. 1 hit.
3.60.110.10. 1 hit.
InterProiView protein in InterPro
IPR003010. C-N_Hydrolase.
IPR014445. Gln-dep_NAD_synthase.
IPR022310. NAD/GMP_synthase.
IPR003694. NAD_synthase.
IPR014729. Rossmann-like_a/b/a_fold.
PANTHERiPTHR23090. PTHR23090. 1 hit.
PfamiView protein in Pfam
PF00795. CN_hydrolase. 1 hit.
PF02540. NAD_synthase. 1 hit.
PIRSFiPIRSF006630. NADS_GAT. 1 hit.
SUPFAMiSSF56317. SSF56317. 1 hit.
PROSITEiView protein in PROSITE
PS50263. CN_HYDROLASE. 1 hit.

Sequencei

Sequence statusi: Complete.

B8IGW6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MFRSLYQHGF ARVAACVGQS HIAEPAANVA EVLALARRCH DQGVAVAVFP
60 70 80 90 100
ELCLSAYAIE DLLLQETLLD GVEAAVATLA EGTRDLLPLL VVGAPLRHGH
110 120 130 140 150
RVYNTAVMLH RGRVLGVVPK SYLPNYREFY EKRHFAPGAG ITGETIRLAG
160 170 180 190 200
AAIPFGTDLL FAAEDLPGLV IGVDVCEDMW IPVPPGALAA LAGATVLVNL
210 220 230 240 250
SGSPITIGRA ESRKLLCQSS SVRCLSAYIY AAAGPGESTT DLAWDGQTAI
260 270 280 290 300
YEDGTLLAES ERFPDGPQVT VADIDLDRLR QERAQMGSFD DNARAVLGER
310 320 330 340 350
PWRRIAFTVA PPRHDLGLAR TVERFPFVPA DPARLAQDCY EGYNIQVAGL
360 370 380 390 400
AQRLRAIRTS KVVIGISGGL DSTHALIVAA KAFDRLGLPR TNILTYTMPG
410 420 430 440 450
FATSAETKGN AHRLMASLGT TATELDIRPA ARQMLTDMGH PFGRGEPVYD
460 470 480 490 500
VTFENVQAGL RTDFLFRLAN QNDAIVVGTG DLSELALGWC TYGVGDQMSH
510 520 530 540 550
YAVNAGVPKT LIQHLIRWVV SSGQFDAAVN RTLEAILATE ISPELVPVEE
560 570 580 590 600
GGQIQSTEAR IGPYALQDFN LFYTLRFGYR PSKIAFLACH AWEDASRGPW
610 620 630 640 650
PPGFPDERRG AYALPEIRAW LKVFLRRFFG FSQFKRSALP NGPKVAAGGA
660 670
LSPRGDWRAP SDGNAAAWLA ELERNVPEA
Length:679
Mass (Da):73,496
Last modified:March 3, 2009 - v1
Checksum:iD8C386F5B930D769
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001349 Genomic DNA. Translation: ACL57841.1.
RefSeqiWP_015929516.1. NC_011894.1.

Genome annotation databases

EnsemblBacteriaiACL57841; ACL57841; Mnod_2890.
KEGGimno:Mnod_2890.

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.

Entry informationi

Entry nameiB8IGW6_METNO
AccessioniPrimary (citable) accession number: B8IGW6
Entry historyiIntegrated into UniProtKB/TrEMBL: March 3, 2009
Last sequence update: March 3, 2009
Last modified: June 7, 2017
This is version 51 of the entry and version 1 of the sequence. See complete history.
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteomeImported