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Protein

Glutamine-dependent NAD(+) synthetase

Gene

nadE

Organism
Methylobacterium nodulans (strain LMG 21967 / CNCM I-2342 / ORS 2060)
Status
Unreviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the ATP-dependent amidation of deamido-NAD to form NAD. Uses L-glutamine as a nitrogen source.UniRule annotation

Catalytic activityi

ATP + deamido-NAD+ + L-glutamine + H2O = AMP + diphosphate + NAD+ + L-glutamate.UniRule annotation

Pathwayi: NAD(+) biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes NAD(+) from deamido-NAD(+) (L-Gln route).UniRule annotation
Proteins known to be involved in this subpathway in this organism are:
  1. Glutamine-dependent NAD(+) synthetase (nadE)
This subpathway is part of the pathway NAD(+) biosynthesis, which is itself part of Cofactor biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes NAD(+) from deamido-NAD(+) (L-Gln route), the pathway NAD(+) biosynthesis and in Cofactor biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei51Proton acceptor; for glutaminase activityUniRule annotation1
Active sitei120For glutaminase activityUniRule annotation1
Binding sitei126L-glutamineUniRule annotation1
Active sitei176Nucleophile; for glutaminase activityUniRule annotation1
Binding sitei203L-glutamineUniRule annotation1
Binding sitei209L-glutamineUniRule annotation1
Binding sitei455Deamido-NADUniRule annotation1
Binding sitei479ATPUniRule annotation1
Binding sitei484Deamido-NADUniRule annotation1
Binding sitei635Deamido-NADUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi365 – 372ATPUniRule annotation8
Nucleotide bindingi489 – 492Deamido-NADUniRule annotation4

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionLigaseUniRule annotationSAAS annotation
LigandATP-bindingUniRule annotationSAAS annotation, NADUniRule annotationSAAS annotation, Nucleotide-binding

Enzyme and pathway databases

BioCyciMNOD460265:GCZK-2819-MONOMER
UniPathwayiUPA00253; UER00334

Names & Taxonomyi

Protein namesi
Recommended name:
Glutamine-dependent NAD(+) synthetaseUniRule annotation (EC:6.3.5.1UniRule annotation)
Alternative name(s):
NAD(+) synthase [glutamine-hydrolyzing]UniRule annotation
Gene namesi
Name:nadEUniRule annotation
Ordered Locus Names:Mnod_2890Imported
OrganismiMethylobacterium nodulans (strain LMG 21967 / CNCM I-2342 / ORS 2060)Imported
Taxonomic identifieri460265 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesMethylobacteriaceaeMethylobacterium
Proteomesi
  • UP000008207 Componenti: Chromosome

Interactioni

Protein-protein interaction databases

STRINGi460265.Mnod_2890

Structurei

3D structure databases

ProteinModelPortaliB8IGW6
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini11 – 276CN hydrolaseInterPro annotationAdd BLAST266

Sequence similaritiesi

In the C-terminal section; belongs to the NAD synthetase family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4108E9H Bacteria
COG0171 LUCA
COG0388 LUCA
HOGENOMiHOG000051304
KOiK01950
OMAiRLAQDCY
OrthoDBiPOG091H026O

Family and domain databases

CDDicd00553 NAD_synthase, 1 hit
Gene3Di3.40.50.620, 1 hit
3.60.110.10, 1 hit
HAMAPiMF_02090 NadE_glutamine_dep, 1 hit
InterProiView protein in InterPro
IPR003010 C-N_Hydrolase
IPR036526 C-N_Hydrolase_sf
IPR014445 Gln-dep_NAD_synthase
IPR022310 NAD/GMP_synthase
IPR003694 NAD_synthase
IPR014729 Rossmann-like_a/b/a_fold
PANTHERiPTHR23090 PTHR23090, 1 hit
PfamiView protein in Pfam
PF00795 CN_hydrolase, 1 hit
PF02540 NAD_synthase, 1 hit
PIRSFiPIRSF006630 NADS_GAT, 1 hit
SUPFAMiSSF56317 SSF56317, 1 hit
PROSITEiView protein in PROSITE
PS50263 CN_HYDROLASE, 1 hit

Sequencei

Sequence statusi: Complete.

B8IGW6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MFRSLYQHGF ARVAACVGQS HIAEPAANVA EVLALARRCH DQGVAVAVFP
60 70 80 90 100
ELCLSAYAIE DLLLQETLLD GVEAAVATLA EGTRDLLPLL VVGAPLRHGH
110 120 130 140 150
RVYNTAVMLH RGRVLGVVPK SYLPNYREFY EKRHFAPGAG ITGETIRLAG
160 170 180 190 200
AAIPFGTDLL FAAEDLPGLV IGVDVCEDMW IPVPPGALAA LAGATVLVNL
210 220 230 240 250
SGSPITIGRA ESRKLLCQSS SVRCLSAYIY AAAGPGESTT DLAWDGQTAI
260 270 280 290 300
YEDGTLLAES ERFPDGPQVT VADIDLDRLR QERAQMGSFD DNARAVLGER
310 320 330 340 350
PWRRIAFTVA PPRHDLGLAR TVERFPFVPA DPARLAQDCY EGYNIQVAGL
360 370 380 390 400
AQRLRAIRTS KVVIGISGGL DSTHALIVAA KAFDRLGLPR TNILTYTMPG
410 420 430 440 450
FATSAETKGN AHRLMASLGT TATELDIRPA ARQMLTDMGH PFGRGEPVYD
460 470 480 490 500
VTFENVQAGL RTDFLFRLAN QNDAIVVGTG DLSELALGWC TYGVGDQMSH
510 520 530 540 550
YAVNAGVPKT LIQHLIRWVV SSGQFDAAVN RTLEAILATE ISPELVPVEE
560 570 580 590 600
GGQIQSTEAR IGPYALQDFN LFYTLRFGYR PSKIAFLACH AWEDASRGPW
610 620 630 640 650
PPGFPDERRG AYALPEIRAW LKVFLRRFFG FSQFKRSALP NGPKVAAGGA
660 670
LSPRGDWRAP SDGNAAAWLA ELERNVPEA
Length:679
Mass (Da):73,496
Last modified:March 3, 2009 - v1
Checksum:iD8C386F5B930D769
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001349 Genomic DNA Translation: ACL57841.1
RefSeqiWP_015929516.1, NC_011894.1

Genome annotation databases

EnsemblBacteriaiACL57841; ACL57841; Mnod_2890
KEGGimno:Mnod_2890

Similar proteinsi

Entry informationi

Entry nameiB8IGW6_METNO
AccessioniPrimary (citable) accession number: B8IGW6
Entry historyiIntegrated into UniProtKB/TrEMBL: March 3, 2009
Last sequence update: March 3, 2009
Last modified: March 28, 2018
This is version 56 of the entry and version 1 of the sequence. See complete history.
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteomeImported
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Main funding by: National Institutes of Health