B8IEM1 (PROA_METNO) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Gamma-glutamyl phosphate reductase
Short name=GPR
EC=1.2.1.41

Alternative name(s):
Glutamate-5-semialdehyde dehydrogenase
Glutamyl-gamma-semialdehyde dehydrogenase
Short name=GSA dehydrogenase

Gene names

Name:	proA
Ordered Locus Names:	Mnod_4728

Organism

Methylobacterium nodulans (strain ORS2060 / LMG 21967) [Complete proteome] [HAMAP]

Taxonomic identifier

460265 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Methylobacteriaceae › Methylobacterium

Protein attributes

Sequence length	431 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the NADPH dependent reduction of L-gamma-glutamyl 5-phosphate into L-glutamate 5-semialdehyde and phosphate. The product spontaneously undergoes cyclization to form 1-pyrroline-5-carboxylate By similarity. HAMAP MF_00412
Catalytic activity	L-glutamate 5-semialdehyde + phosphate + NADP+ = L-glutamyl 5-phosphate + NADPH. HAMAP MF_00412
Pathway	Amino-acid biosynthesis; L-proline biosynthesis; L-glutamate 5-semialdehyde from L-glutamate: step 2/2. HAMAP MF_00412
Subcellular location	Cytoplasm By similarity HAMAP MF_00412.
Sequence similarities	Belongs to the gamma-glutamyl phosphate reductase family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Proline biosynthesis
Cellular component	Cytoplasm
Ligand	NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	proline biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	NADP binding Inferred from electronic annotation. Source: InterPro glutamate-5-semialdehyde dehydrogenase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 431	431	Gamma-glutamyl phosphate reductase HAMAP MF_00412		PRO_1000193623

Sequences

	Sequence		Length	Mass (Da)
	B8IEM1 [UniParc]. Last modified March 3, 2009. Version 1. Checksum: ED523112C512FD03	FASTA	431	44,840
	10 20 30 40 50 60 MSVLTLKARA GADDVDALMR EIGRQARAAA RRMALVSGQQ KDRGLRAAAA AIRAAAPAIL 70 80 90 100 110 120 EANRADLTEA RAKDLPAATL DRLELTPARV EAIAAAVEAI AGLPDPVGRR LAAFERPNGL 130 140 150 160 170 180 SIERIATPLG VVGVIFESRP NVTADAGALC LKAGNAAVLR AGSESLRSAA AIAAAMTEGL 190 200 210 220 230 240 VAEGLPADAI QLVPTRDRAA VGAMLSGLDG CIDVIVPRGG KNLVARVQNE AKVPVFAHLE 250 260 270 280 290 300 GICHVFVHAA ADLAMARTIV RNSKLRRTGI CGAAETLLVD RACASTHLAP LVLDLLEAGC 310 320 330 340 350 360 AVRGDEATRA VDPRVTPASE ADWRTEYLDA VIAVRVVEGL DAAIDHIETF GSHHTDAIVT 370 380 390 400 410 420 HDTGAAERFL AEVDSAIVVH NASTQFADGG EFGFGAEIGI ATGRMHARGP VGVEQLTTFK 430 YRVHGSGQIR P

References

[1]

"Complete sequence of chromosome of Methylobacterium nodulans ORS 2060." Lucas S., Copeland A., Lapidus A., Glavina del Rio T., Dalin E., Tice H., Bruce D., Goodwin L., Pitluck S., Sims D., Brettin T., Detter J.C., Han C., Larimer F., Land M., Hauser L., Kyrpides N., Ivanova N., Marx C.J., Richardson P. Submitted (JAN-2009) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ORS2060 / LMG 21967.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP001349 Genomic DNA. Translation: ACL59593.1.
RefSeq	YP_002499896.1. NC_011894.1.
3D structure databases
ProteinModelPortal	B8IEM1.
ModBase	Search...
Protein-protein interaction databases
STRING	B8IEM1.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	7307720.
GenomeReviews	Gene locus Mnod_4728 in contig CP001349_GR.
KEGG	mno:Mnod_4728.
PATRIC	22551298. VBIMetNod76414_5353.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG318080.
OMA	YGICGAM.
ProtClustDB	PRK00197.
Family and domain databases
HAMAP	MF_00412. ProA. [Tree]
InterPro	IPR016161. Ald_DH/histidinol_DH. IPR016163. Ald_DH_C. IPR016162. Ald_DH_N. IPR015590. Aldehyde_DH_dom. IPR000965. G-glutamylP_reductase. IPR020593. G-glutamylP_reductase_CS. IPR012134. Glu-5-SA_DH. [Graphical view]
Gene3D	G3DSA:3.40.309.10. Aldehyde_dehydrogenase_C. 1 hit. G3DSA:3.40.605.10. Aldehyde_dehydrogenase_N. 2 hits.
KO	K00147.
PANTHER	PTHR11063:SF1. GSA_DH. 1 hit.
Pfam	PF00171. Aldedh. 1 hit. [Graphical view]
PIRSF	PIRSF000151. GPR. 1 hit.
SUPFAM	SSF53720. Aldehyde_DH/Histidinol_DH. 1 hit.
TIGRFAMs	TIGR00407. ProA. 1 hit.
PROSITE	PS01223. PROA. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PROA_METNO

Accession

Primary (citable) accession number: B8IEM1

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 28, 2009
Last sequence update:	March 3, 2009
Last modified:	January 25, 2012
This is version 27 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program