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B8IDD6

- LIPA_METNO

UniProt

B8IDD6 - LIPA_METNO

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Protein
Lipoyl synthase
Gene
lipA, Mnod_6533
Organism
Methylobacterium nodulans (strain ORS2060 / LMG 21967)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes the radical-mediated insertion of two sulfur atoms into the C-6 and C-8 positions of the octanoyl moiety bound to the lipoyl domains of lipoate-dependent enzymes, thereby converting the octanoylated domains into lipoylated derivatives By similarity.UniRule annotation

Catalytic activityi

Protein N(6)-(octanoyl)lysine + 2 sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine = protein N(6)-(lipoyl)lysine + 2 (sulfur carrier) + 2 L-methionine + 2 5'-deoxyadenosine.UniRule annotation

Cofactori

Binds 2 4Fe-4S clusters per subunit. One cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine By similarity.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi60 – 601Iron-sulfur 1 (4Fe-4S) By similarity
Metal bindingi65 – 651Iron-sulfur 1 (4Fe-4S) By similarity
Metal bindingi71 – 711Iron-sulfur 1 (4Fe-4S) By similarity
Metal bindingi86 – 861Iron-sulfur 2 (4Fe-4S-S-AdoMet) By similarity
Metal bindingi90 – 901Iron-sulfur 2 (4Fe-4S-S-AdoMet) By similarity
Metal bindingi93 – 931Iron-sulfur 2 (4Fe-4S-S-AdoMet) By similarity

GO - Molecular functioni

  1. 4 iron, 4 sulfur cluster binding Source: UniProtKB-HAMAP
  2. lipoate synthase activity Source: UniProtKB-HAMAP
  3. metal ion binding Source: UniProtKB-KW
Complete GO annotation...

GO - Biological processi

  1. protein lipoylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Ligandi

4Fe-4S, Iron, Iron-sulfur, Metal-binding, S-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciMNOD460265:GCZK-6605-MONOMER.
UniPathwayiUPA00538; UER00593.

Names & Taxonomyi

Protein namesi
Recommended name:
Lipoyl synthase (EC:2.8.1.8)
Alternative name(s):
Lip-syn
Short name:
LS
Lipoate synthase
Lipoic acid synthase
Sulfur insertion protein LipA
Gene namesi
Name:lipA
Ordered Locus Names:Mnod_6533
OrganismiMethylobacterium nodulans (strain ORS2060 / LMG 21967)
Taxonomic identifieri460265 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesMethylobacteriaceaeMethylobacterium
ProteomesiUP000008207: Chromosome

Subcellular locationi

Cytoplasm Reviewed prediction UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 320320Lipoyl synthaseUniRule annotation
PRO_1000124639Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi460265.Mnod_6533.

Structurei

3D structure databases

ProteinModelPortaliB8IDD6.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0320.
HOGENOMiHOG000235997.
KOiK03644.
OMAiPEEPYNT.
OrthoDBiEOG6038ZS.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
HAMAPiMF_00206. Lipoyl_synth.
InterProiIPR013785. Aldolase_TIM.
IPR006638. Elp3/MiaB/NifB.
IPR003698. Lipoyl_synth.
IPR007197. rSAM.
[Graphical view]
PANTHERiPTHR10949. PTHR10949. 1 hit.
PfamiPF04055. Radical_SAM. 1 hit.
[Graphical view]
PIRSFiPIRSF005963. Lipoyl_synth. 1 hit.
SMARTiSM00729. Elp3. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00510. lipA. 1 hit.

Sequencei

Sequence statusi: Complete.

B8IDD6-1 [UniParc]FASTAAdd to Basket

« Hide

MAVVLDLLAN DARPRHPEKA HRPDQPIQRK PDWIRVRAPG SPGWAETNRI    50
VREHGLVTVC EEAGCPNIGE CWEKKHATFM IMGDTCTRAC AFCNVRTGLP 100
QGLDGDEPAR VADAVARLGL SHVVITSVDR DDLRDGGAGH FAAVIGAIRQ 150
ASPGTTIEVL TPDFLRKEGA LDVVVAAKPD VFNHNLETVP SKYLTVRPGA 200
RYFHSVRLLQ RVKELDPTMF TKSGIMVGLG EERNEVLQLM DDLRSAEVDF 250
LTIGQYLQPT KKHHPVKRFV PPDEFRAYET TAYAKGFLLV SATPLTRSSH 300
HAGADFARLK QARLDRLATA 320
Length:320
Mass (Da):35,337
Last modified:March 3, 2009 - v1
Checksum:i6B4A886D162D66D3
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP001349 Genomic DNA. Translation: ACL61302.1.
RefSeqiWP_015932875.1. NC_011894.1.
YP_002501605.1. NC_011894.1.

Genome annotation databases

EnsemblBacteriaiACL61302; ACL61302; Mnod_6533.
GeneIDi7307311.
KEGGimno:Mnod_6533.
PATRICi22554807. VBIMetNod76414_7082.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP001349 Genomic DNA. Translation: ACL61302.1 .
RefSeqi WP_015932875.1. NC_011894.1.
YP_002501605.1. NC_011894.1.

3D structure databases

ProteinModelPortali B8IDD6.
ModBasei Search...

Protein-protein interaction databases

STRINGi 460265.Mnod_6533.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ACL61302 ; ACL61302 ; Mnod_6533 .
GeneIDi 7307311.
KEGGi mno:Mnod_6533.
PATRICi 22554807. VBIMetNod76414_7082.

Phylogenomic databases

eggNOGi COG0320.
HOGENOMi HOG000235997.
KOi K03644.
OMAi PEEPYNT.
OrthoDBi EOG6038ZS.

Enzyme and pathway databases

UniPathwayi UPA00538 ; UER00593 .
BioCyci MNOD460265:GCZK-6605-MONOMER.

Family and domain databases

Gene3Di 3.20.20.70. 1 hit.
HAMAPi MF_00206. Lipoyl_synth.
InterProi IPR013785. Aldolase_TIM.
IPR006638. Elp3/MiaB/NifB.
IPR003698. Lipoyl_synth.
IPR007197. rSAM.
[Graphical view ]
PANTHERi PTHR10949. PTHR10949. 1 hit.
Pfami PF04055. Radical_SAM. 1 hit.
[Graphical view ]
PIRSFi PIRSF005963. Lipoyl_synth. 1 hit.
SMARTi SM00729. Elp3. 1 hit.
[Graphical view ]
TIGRFAMsi TIGR00510. lipA. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "Complete sequence of chromosome of Methylobacterium nodulans ORS 2060."
    US DOE Joint Genome Institute
    Lucas S., Copeland A., Lapidus A., Glavina del Rio T., Dalin E., Tice H., Bruce D., Goodwin L., Pitluck S., Sims D., Brettin T., Detter J.C., Han C., Larimer F., Land M., Hauser L., Kyrpides N., Ivanova N., Marx C.J., Richardson P.
    Submitted (JAN-2009) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ORS2060 / LMG 21967.

Entry informationi

Entry nameiLIPA_METNO
AccessioniPrimary (citable) accession number: B8IDD6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: March 3, 2009
Last modified: September 3, 2014
This is version 40 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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