B8ID77 (B8ID77_METNO) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names

Recommended name:
Glutamate--tRNA ligase 2 HAMAP MF_00022
EC=6.1.1.17 HAMAP MF_00022

Alternative name(s):
Glutamyl-tRNA synthetase 2 HAMAP MF_00022

Gene names

Name:	gltX2 HAMAP MF_00022
Ordered Locus Names:	Mnod_4602

Organism

Methylobacterium nodulans (strain ORS2060 / LMG 21967) [Complete proteome] [HAMAP]

Taxonomic identifier

460265 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Methylobacteriaceae › Methylobacterium

Protein attributes

Sequence length	447 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction: glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu) By similarity. HAMAP MF_00022
Catalytic activity	ATP + L-glutamate + tRNA(Glu) = AMP + diphosphate + L-glutamyl-tRNA(Glu). HAMAP MF_00022
Subunit structure	Monomer By similarity. HAMAP MF_00022
Subcellular location	Cytoplasm By similarity HAMAP MF_00022.
Sequence similarities	Belongs to the class-I aminoacyl-tRNA synthetase family. HAMAP MF_00022 RuleBase RU003489

Ontologies

Keywords
Biological process	Protein biosynthesis HAMAP MF_00022 RuleBase RU003489
Cellular component	Cytoplasm HAMAP MF_00022
Ligand	ATP-binding HAMAP MF_00022 RuleBase RU003489 Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase HAMAP MF_00022 RuleBase RU003489 EMBL ACL59469.1 Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glutamyl-tRNA aminoacylation Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP glutamate-tRNA ligase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Regions
	Motif	9 – 19	11	"HIGH" region By similarity HAMAP MF_00022
	Motif	240 – 244	5	"KMSKS" region By similarity HAMAP MF_00022
Sites
	Binding site	243	1	ATP By similarity HAMAP MF_00022

Sequences

	Sequence		Length	Mass (Da)
	B8ID77 [UniParc]. Last modified March 3, 2009. Version 1. Checksum: 161878CAAD7F3FED	FASTA	447	48,477
	10 20 30 40 50 60 MAPVVRFAPS PTGYLHIGNA RTALFNALFA RREGGTFVLR LDDTDTARST PEFAAAIAED 70 80 90 100 110 120 LRWLGIAPDR SVRQSDRIAL YDAAAERLRA SGRLYPAYET PDELERRRRR QLGRGQPPVY 130 140 150 160 170 180 DRAALRLTQA ERTALEAEGR RPHWRFRLDP GPVTWTDLVR GPCHVEAESL SDPVLVREDG 190 200 210 220 230 240 SYLYTLPSVV DDAEMGITHV IRGEDHVTNT AVQIQIFAAL GAPVPVFAHH NLLTTASGEG 250 260 270 280 290 300 LSKRLGHLSL RGLRADGYEP GAVASLAVLT GSAEAVRAVA DLDELARLLD LAHVSRAPAK 310 320 330 340 350 360 FDPHDLDQLN ARIIHGMDAA EALPRLAALG IPPARAEAFW TAVRANLTKL SDAAAWWRVV 370 380 390 400 410 420 EGPVMPVVAD AAFAAQAAAL LPPEPWDGGT WKAWGDAVKA RIGLKGKALF LPLRLALTGL 430 440 DHGPDLSGLL PLIGRERALR RLRGEAA

References

[1]

"Complete sequence of chromosome of Methylobacterium nodulans ORS 2060." Lucas S., Copeland A., Lapidus A., Glavina del Rio T., Dalin E., Tice H., Bruce D., Goodwin L., Pitluck S., Sims D., Brettin T., Detter J.C., Han C., Larimer F., Land M., Hauser L., Kyrpides N., Ivanova N., Marx C.J., Richardson P. Submitted (JAN-2009) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ORS2060 / LMG 21967.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP001349 Genomic DNA. Translation: ACL59469.1.
RefSeq	YP_002499772.1. NC_011894.1.
3D structure databases
ProteinModelPortal	B8ID77.
ModBase	Search...
Protein-protein interaction databases
STRING	B8ID77.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	7303698.
GenomeReviews	Gene locus Mnod_4602 in contig CP001349_GR.
KEGG	mno:Mnod_4602.
PATRIC	22551044. VBIMetNod76414_5226.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG628189.
OMA	DQERIEW.
ProtClustDB	PRK12558.
Family and domain databases
HAMAP	MF_00022_B. Glu_tRNA_synth_B. [Tree]
InterPro	IPR008925. aa-tRNA-synth_I_codon-bd. IPR020751. aa-tRNA-synth_I_codon-bd_sub2. IPR001412. aa-tRNA-synth_I_CS. IPR004527. Glu-tRNA-synth_Ib_bac/mito. IPR000924. Glu/Gln-tRNA-synth_Ib. IPR020061. Glu/Gln-tRNA-synth_Ib_a-bdl. IPR020058. Glu/Gln-tRNA-synth_Ib_cat-dom. IPR014729. Rossmann-like_a/b/a_fold. [Graphical view]
Gene3D	G3DSA:1.10.1160.10. Glu/Gln-tRNA-synth_Ic_a-bdl. 1 hit. G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 2 hits. G3DSA:1.10.10.350. tRNA_synt_bd. 1 hit.
KO	K01885.
PANTHER	PTHR10119. Glu_tRNA-synt_1c. 1 hit. PTHR10119:SF1. PTHR10119:SF1. 1 hit.
Pfam	PF00749. tRNA-synt_1c. 1 hit. [Graphical view]
PRINTS	PR00987. TRNASYNTHGLU.
SUPFAM	SSF48163. tRNA-synt_bind. 1 hit.
TIGRFAMs	TIGR00464. GltX_bact. 1 hit.
PROSITE	PS00178. AA_TRNA_LIGASE_I. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

B8ID77_METNO

Accession

Primary (citable) accession number: B8ID77

Entry history

Integrated into UniProtKB/TrEMBL:	March 3, 2009
Last sequence update:	March 3, 2009
Last modified:	January 25, 2012
This is version 29 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)