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B8I814 (TRUA_CLOCE) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 35. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
tRNA pseudouridine synthase A

EC=5.4.99.12
Alternative name(s):
tRNA pseudouridine(38-40) synthase
tRNA pseudouridylate synthase I
tRNA-uridine isomerase I
Gene names
Name:truA
Ordered Locus Names:Ccel_0793
OrganismClostridium cellulolyticum (strain ATCC 35319 / DSM 5812 / JCM 6584 / H10) [Complete proteome] [HAMAP]
Taxonomic identifier394503 [NCBI]
Taxonomic lineageBacteriaFirmicutesClostridiaClostridialesClostridiaceaeClostridium

Protein attributes

Sequence length245 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Formation of pseudouridine at positions 38, 39 and 40 in the anticodon stem and loop of transfer RNAs By similarity. HAMAP-Rule MF_00171

Catalytic activity

tRNA uridine(38-40) = tRNA pseudouridine(38-40). HAMAP-Rule MF_00171

Subunit structure

Homodimer By similarity. HAMAP-Rule MF_00171

Sequence similarities

Belongs to the tRNA pseudouridine synthase TruA family.

Ontologies

Keywords
   Biological processtRNA processing
   Molecular functionIsomerase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processtRNA pseudouridine synthesis

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Molecular_functionRNA binding

Inferred from electronic annotation. Source: InterPro

pseudouridine synthase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 245245tRNA pseudouridine synthase A HAMAP-Rule MF_00171
PRO_1000194540

Sites

Active site521Nucleophile By similarity
Binding site1101Substrate By similarity

Sequences

Sequence LengthMass (Da)Tools
B8I814 [UniParc].

Last modified March 3, 2009. Version 1.
Checksum: 9121A8E97EBD71C2

FASTA24527,417
        10         20         30         40         50         60 
MRKIRLTIEY DGTNYHGWQI QKNAKTVQEV IQKALSKLLG EDVGVTGCSR TDVGVHAYGQ 

        70         80         90        100        110        120 
VAHFLTDSKI PGDKFSYAIN NLLPDDIVIK NSEEVPEEFH ARYSAKGKKY RYLIYNSPHA 

       130        140        150        160        170        180 
SAIMRNRSCH VRPELNVAEM QKAAGYFKGE HDFLAFQATG GQVRSTVREI YGMEVYVKED 

       190        200        210        220        230        240 
NMISIEVSGN GFLYNMVRII AGTLIYVGMG KLHESEIPGI IAGLDRTKAG KTAPAQGLYL 


VQIYY 

« Hide

References

[1]"Complete sequence of Clostridium cellulolyticum H10."
US DOE Joint Genome Institute
Lucas S., Copeland A., Lapidus A., Glavina del Rio T., Dalin E., Tice H., Bruce D., Goodwin L., Pitluck S., Chertkov O., Saunders E., Brettin T., Detter J.C., Han C., Larimer F., Land M., Hauser L., Kyrpides N. expand/collapse author list , Ivanova N., Zhou J., Richardson P.
Submitted (JAN-2009) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 35319 / DSM 5812 / JCM 6584 / H10.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP001348 Genomic DNA. Translation: ACL75171.1.
RefSeqYP_002505151.1. NC_011898.1.

3D structure databases

ProteinModelPortalB8I814.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING394503.Ccel_0793.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaACL75171; ACL75171; Ccel_0793.
GeneID7309646.
KEGGcce:Ccel_0793.
PATRIC19432395. VBICloCel57783_0821.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0101.
HOGENOMHOG000248673.
KOK06173.
OMAHITADRF.
OrthoDBEOG6Z9B4R.
ProtClustDBPRK00021.

Enzyme and pathway databases

BioCycCCEL394503:GJET-818-MONOMER.

Family and domain databases

Gene3D3.30.70.580. 1 hit.
3.30.70.660. 1 hit.
HAMAPMF_00171. TruA.
InterProIPR020103. PsdUridine_synth_cat_dom.
IPR001406. PsdUridine_synth_TruA.
IPR020097. PsdUridine_synth_TruA_a/b_dom.
IPR020095. PsdUridine_synth_TruA_C.
IPR020094. PsdUridine_synth_TruA_N.
[Graphical view]
PANTHERPTHR11142. PTHR11142. 1 hit.
PfamPF01416. PseudoU_synth_1. 2 hits.
[Graphical view]
PIRSFPIRSF001430. tRNA_psdUrid_synth. 1 hit.
SUPFAMSSF55120. SSF55120. 1 hit.
TIGRFAMsTIGR00071. hisT_truA. 1 hit.
ProtoNetSearch...

Entry information

Entry nameTRUA_CLOCE
AccessionPrimary (citable) accession number: B8I814
Entry history
Integrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: March 3, 2009
Last modified: February 19, 2014
This is version 35 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families