B8I6R1 (ASNA_CLOCE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Aspartate--ammonia ligase
EC=6.3.1.1

Alternative name(s):
Asparagine synthetase A

Gene names

Name:	asnA
Ordered Locus Names:	Ccel_2575

Organism

Clostridium cellulolyticum (strain ATCC 35319 / DSM 5812 / JCM 6584 / H10) [Complete proteome] [HAMAP]

Taxonomic identifier

394503 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Clostridia › Clostridiales › Clostridiaceae › Clostridium

Protein attributes

Sequence length	336 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + L-aspartate + NH3 = AMP + diphosphate + L-asparagine. HAMAP MF_00555
Pathway	Amino-acid biosynthesis; L-asparagine biosynthesis; L-asparagine from L-aspartate (ammonia route): step 1/1. HAMAP MF_00555
Subcellular location	Cytoplasm By similarity HAMAP MF_00555.
Sequence similarities	Belongs to the class-II aminoacyl-tRNA synthetase family. AsnA subfamily.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Asparagine biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	asparagine biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW tRNA aminoacylation for protein translation Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW aminoacyl-tRNA ligase activity Inferred from electronic annotation. Source: InterPro aspartate-ammonia ligase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 336	336	Aspartate--ammonia ligase HAMAP MF_00555		PRO_1000146691

Sequences

	Sequence		Length	Mass (Da)
	B8I6R1 [UniParc]. Last modified March 3, 2009. Version 1. Checksum: 50F85EA5C57D3A3A	FASTA	336	38,411
	10 20 30 40 50 60 MNSLIKPEGY KPSLDIRMTE VAIKKTKDYF ESELAKELNL TRVSAPLFVR PETGLNDNLN 70 80 90 100 110 120 GVERPVAFDV KGIGGDTVEV VHSLAKWKRM ALKRYGFAPG EGLYTDMNAI RRDEDMDNLH 130 140 150 160 170 180 SVYVDQWDWE QIILKSERTE ETLKTIVSKI FSVFKRTENY LSGLYPNLQK YLPEDIFFVT 190 200 210 220 230 240 TQELEDMYPE LTPKERETAL AKEKKAIFIM KIGDTLKSGI KHDGRAPDYD DWALNGDIVF 250 260 270 280 290 300 YYPVLDLAYE VSSMGIRVDE DSLVAQLKKA GCEDRKNLKF HKELINKELP YTIGGGIGQS 310 320 330 RICMFFLGKA HIGEVQASIW SDDMISECSQ HNIQLL

References

[1]

"Complete sequence of Clostridium cellulolyticum H10." US DOE Joint Genome Institute Lucas S., Copeland A., Lapidus A., Glavina del Rio T., Dalin E., Tice H., Bruce D., Goodwin L., Pitluck S., Chertkov O., Saunders E., Brettin T., Detter J.C., Han C., Larimer F., Land M., Hauser L., Kyrpides N. , Ivanova N., Zhou J., Richardson P. Submitted (JAN-2009) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 35319 / DSM 5812 / JCM 6584 / H10.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP001348 Genomic DNA. Translation: ACL76903.1.
RefSeq	YP_002506883.1. NC_011898.1.
3D structure databases
ProteinModelPortal	B8I6R1.
ModBase	Search...
Protein-protein interaction databases
STRING	B8I6R1.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	7311228.
GenomeReviews	Gene locus Ccel_2575 in contig CP001348_GR.
KEGG	cce:Ccel_2575.
PATRIC	19436123. VBICloCel57783_2650.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG288146.
OMA	LNDNLNG.
ProtClustDB	PRK05425.
Family and domain databases
HAMAP	MF_00555. AsnA. [Tree]
InterPro	IPR006195. aa-tRNA-synth_II. IPR004618. AsnA. [Graphical view]
KO	K01914.
Pfam	PF03590. AsnA. 1 hit. [Graphical view]
PIRSF	PIRSF001555. Asp_ammon_ligase. 1 hit.
TIGRFAMs	TIGR00669. AsnA. 1 hit.
PROSITE	PS50862. AA_TRNA_LIGASE_II. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ASNA_CLOCE

Accession

Primary (citable) accession number: B8I6R1

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 28, 2009
Last sequence update:	March 3, 2009
Last modified:	January 25, 2012
This is version 21 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program