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Protein

Glutamyl-tRNA(Gln) amidotransferase subunit A

Gene

gatA

Organism
Clostridium cellulolyticum (strain ATCC 35319 / DSM 5812 / JCM 6584 / H10)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Allows the formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu-tRNA(Gln) in organisms which lack glutaminyl-tRNA synthetase. The reaction takes place in the presence of glutamine and ATP through an activated gamma-phospho-Glu-tRNA(Gln).UniRule annotation

Catalytic activityi

ATP + L-glutamyl-tRNA(Gln) + L-glutamine = ADP + phosphate + L-glutaminyl-tRNA(Gln) + L-glutamate.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei78 – 781Charge relay systemUniRule annotation
Active sitei153 – 1531Charge relay systemUniRule annotation
Active sitei177 – 1771Acyl-ester intermediateUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciCCEL394503:GJET-2493-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Glutamyl-tRNA(Gln) amidotransferase subunit AUniRule annotation (EC:6.3.5.7UniRule annotation)
Short name:
Glu-ADT subunit AUniRule annotation
Gene namesi
Name:gatAUniRule annotation
Ordered Locus Names:Ccel_2436
OrganismiClostridium cellulolyticum (strain ATCC 35319 / DSM 5812 / JCM 6584 / H10)
Taxonomic identifieri394503 [NCBI]
Taxonomic lineageiBacteriaFirmicutesClostridiaClostridialesRuminococcaceaeRuminiclostridium
Proteomesi
  • UP000001349 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 486486Glutamyl-tRNA(Gln) amidotransferase subunit APRO_1000122475Add
BLAST

Interactioni

Subunit structurei

Heterotrimer of A, B and C subunits.UniRule annotation

Protein-protein interaction databases

STRINGi394503.Ccel_2436.

Structurei

3D structure databases

ProteinModelPortaliB8I601.
SMRiB8I601. Positions 1-485.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the amidase family. GatA subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105C3P. Bacteria.
COG0154. LUCA.
HOGENOMiHOG000116699.
KOiK02433.
OMAiHASPLEM.
OrthoDBiPOG091H00O9.

Family and domain databases

Gene3Di3.90.1300.10. 1 hit.
HAMAPiMF_00120. GatA. 1 hit.
InterProiIPR000120. Amidase.
IPR020556. Amidase_CS.
IPR023631. Amidase_dom.
IPR004412. GatA.
[Graphical view]
PANTHERiPTHR11895. PTHR11895. 1 hit.
PfamiPF01425. Amidase. 1 hit.
[Graphical view]
SUPFAMiSSF75304. SSF75304. 1 hit.
TIGRFAMsiTIGR00132. gatA. 1 hit.
PROSITEiPS00571. AMIDASES. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

B8I601-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDITRLTIKE ARKLLDNREI SALELTRTYL DRINTLDGKV ESYLSVTEDM
60 70 80 90 100
ALKQAEQAQN LIDYGKASLL TGIPLSIKDN ICIEGTKTTC ASKMLEDFVS
110 120 130 140 150
PYTATAVDKL FADNAVILGK TNLDEFAMGG STENSAFKIT KNPFDLTRVP
160 170 180 190 200
GGSSGGSAAC VSASLALGSL GSDTGGSVRQ PASFCGVVGM KPTYGLVSRY
210 220 230 240 250
GLVAFASSFD QIGPIAKTVE DCAIILDSIC GNDPKDATSL KYENDSSYSS
260 270 280 290 300
SVSGDIKGFK FGLPKEYLTE GLNAEVKESL YKSIDKLESM GAKIEEFSMP
310 320 330 340 350
VLKHAVPAYY LMSSAEASSN LSRYDGVKYG YRADNCKSFN ELIGKSRAEG
360 370 380 390 400
FGKEVKRRIL LGTYSLASGY YDAYYKKALK LRTLISNGFN EAFTKYDVVL
410 420 430 440 450
HPTTPETAFK IGQNSNSPLA MYLADIYTVA VNIAGLPSIS LPCGYDSNGL
460 470 480
PIGLSFTGKP LGEKDIFRAA ASFEAEFSDK FRVPAI
Length:486
Mass (Da):52,399
Last modified:March 3, 2009 - v1
Checksum:i64BBFA048A605F56
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001348 Genomic DNA. Translation: ACL76766.1.
RefSeqiWP_015925855.1. NC_011898.1.

Genome annotation databases

EnsemblBacteriaiACL76766; ACL76766; Ccel_2436.
KEGGicce:Ccel_2436.
PATRICi19435845. VBICloCel57783_2511.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001348 Genomic DNA. Translation: ACL76766.1.
RefSeqiWP_015925855.1. NC_011898.1.

3D structure databases

ProteinModelPortaliB8I601.
SMRiB8I601. Positions 1-485.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi394503.Ccel_2436.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiACL76766; ACL76766; Ccel_2436.
KEGGicce:Ccel_2436.
PATRICi19435845. VBICloCel57783_2511.

Phylogenomic databases

eggNOGiENOG4105C3P. Bacteria.
COG0154. LUCA.
HOGENOMiHOG000116699.
KOiK02433.
OMAiHASPLEM.
OrthoDBiPOG091H00O9.

Enzyme and pathway databases

BioCyciCCEL394503:GJET-2493-MONOMER.

Family and domain databases

Gene3Di3.90.1300.10. 1 hit.
HAMAPiMF_00120. GatA. 1 hit.
InterProiIPR000120. Amidase.
IPR020556. Amidase_CS.
IPR023631. Amidase_dom.
IPR004412. GatA.
[Graphical view]
PANTHERiPTHR11895. PTHR11895. 1 hit.
PfamiPF01425. Amidase. 1 hit.
[Graphical view]
SUPFAMiSSF75304. SSF75304. 1 hit.
TIGRFAMsiTIGR00132. gatA. 1 hit.
PROSITEiPS00571. AMIDASES. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiGATA_CLOCE
AccessioniPrimary (citable) accession number: B8I601
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: March 3, 2009
Last modified: September 7, 2016
This is version 49 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.