B8I5A9 (ILVD_CLOCE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Dihydroxy-acid dehydratase
Short name=DAD
EC=4.2.1.9

Gene names

Name:	ilvD
Ordered Locus Names:	Ccel_0302

Organism

Clostridium cellulolyticum (strain ATCC 35319 / DSM 5812 / JCM 6584 / H10) [Complete proteome] [HAMAP]

Taxonomic identifier

394503 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Clostridia › Clostridiales › Clostridiaceae › Clostridium

Protein attributes

Sequence length	552 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	2,3-dihydroxy-3-methylbutanoate = 3-methyl-2-oxobutanoate + H2O. HAMAP MF_00012
Cofactor	Binds 1 4Fe-4S cluster Potential.
Pathway	Amino-acid biosynthesis; L-isoleucine biosynthesis; L-isoleucine from 2-oxobutanoate: step 3/4. HAMAP MF_00012 Amino-acid biosynthesis; L-valine biosynthesis; L-valine from pyruvate: step 3/4. HAMAP MF_00012
Sequence similarities	Belongs to the IlvD/Edd family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Branched-chain amino acid biosynthesis
Ligand	4Fe-4S Iron Iron-sulfur Metal-binding
Molecular function	Lyase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	branched chain family amino acid biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	4 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW dihydroxy-acid dehydratase activity Inferred from electronic annotation. Source: EC metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 552	552	Dihydroxy-acid dehydratase HAMAP MF_00012		PRO_1000190659
Sites
	Metal binding	119	1	Iron-sulfur (4Fe-4S) Potential
	Metal binding	191	1	Iron-sulfur (4Fe-4S) Potential

Sequences

	Sequence		Length	Mass (Da)
	B8I5A9 [UniParc]. Last modified March 3, 2009. Version 1. Checksum: D4925533E40E3CCC	FASTA	552	58,548
	10 20 30 40 50 60 MRSDIVKKGI EKAPHRSLFK AMGYTDEELE RPLIGVANSK SEIIPGHIHL DKLTEAVKAG 70 80 90 100 110 120 IRMAGGTPIE FGAIGVCDGI AMGHTGMKYS LATRELIADS CEAMSKAHSF DGMVFIPNCD 130 140 150 160 170 180 KIVPGMLMAA ARINIPSIVI SGGPMLSLNR DGKQLDLNSL FEAVGSYKAG TMTKEEVDDI 190 200 210 220 230 240 EDHACPGCGS CSGMFTANSM NCLTEVLGMG LTGNGTIPAV YAERIRLAKY AGMKIMELVE 250 260 270 280 290 300 KDIKPSDILT NEAFENALTV DMALGCSTNS VLHLPAIANE LGIEINLDII NEISSRTPNL 310 320 330 340 350 360 CKLAPAGKYH IQDLYSAGGV QAVMSELAKK DLLHLDLVTA TGKTIRENIQ NAKVKDYEIV 370 380 390 400 410 420 KSIDTPYSAT GGIAVLRGNI APDGAVVKKS AVAEKMLIHT GPARVFDSED EAITAIYSGQ 430 440 450 460 470 480 INKGDVVIIR YEGPKGGPGM REMLSPTSAI AGMGLDSDVA LITDGRFSGA SRGASIGHVS 490 500 510 520 530 540 PEAMEGGPIA LVQEGDIVDI DIPAGRINIQ VTNEEMVKRK ESWKAPKPKI TTGYLGRYAR 550 LVTSASTGAV LK

References

[1]

"Complete sequence of Clostridium cellulolyticum H10." US DOE Joint Genome Institute Lucas S., Copeland A., Lapidus A., Glavina del Rio T., Dalin E., Tice H., Bruce D., Goodwin L., Pitluck S., Chertkov O., Saunders E., Brettin T., Detter J.C., Han C., Larimer F., Land M., Hauser L., Kyrpides N. , Ivanova N., Zhou J., Richardson P. Submitted (JAN-2009) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 35319 / DSM 5812 / JCM 6584 / H10.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP001348 Genomic DNA. Translation: ACL74689.1.
RefSeq	YP_002504669.1. NC_011898.1.
3D structure databases
ProteinModelPortal	B8I5A9.
ModBase	Search...
Protein-protein interaction databases
STRING	B8I5A9.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	7309194.
GenomeReviews	Gene locus Ccel_0302 in contig CP001348_GR.
KEGG	cce:Ccel_0302.
PATRIC	19431355. VBICloCel57783_0318.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG671001.
OMA	MALVIRY.
ProtClustDB	PRK00911.
Family and domain databases
HAMAP	MF_00012. IlvD. [Tree]
InterPro	IPR015928. Aconitase/3IPM_dehydase_swvl. IPR004404. DihydroxyA_deHydtase. IPR000581. DiOHA_6PGluconate_deHydtase. IPR020558. DiOHA_6PGluconate_deHydtase_CS. [Graphical view]
KO	K01687.
PANTHER	PTHR21000. ILVD_EDD_family. 1 hit.
Pfam	PF00920. ILVD_EDD. 1 hit. [Graphical view]
SUPFAM	SSF52016. Aconitase/3IPM_dehydase_swvl. 1 hit.
TIGRFAMs	TIGR00110. IlvD. 1 hit.
PROSITE	PS00886. ILVD_EDD_1. 1 hit. PS00887. ILVD_EDD_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ILVD_CLOCE

Accession

Primary (citable) accession number: B8I5A9

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 28, 2009
Last sequence update:	March 3, 2009
Last modified:	January 25, 2012
This is version 22 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program