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B8I590 (DDL_CLOCE) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 38. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
D-alanine--D-alanine ligase

EC=6.3.2.4
Alternative name(s):
D-Ala-D-Ala ligase
D-alanylalanine synthetase
Gene names
Name:ddl
Ordered Locus Names:Ccel_0283
OrganismClostridium cellulolyticum (strain ATCC 35319 / DSM 5812 / JCM 6584 / H10) [Complete proteome] [HAMAP]
Taxonomic identifier394503 [NCBI]
Taxonomic lineageBacteriaFirmicutesClostridiaClostridialesClostridiaceaeClostridium

Protein attributes

Sequence length376 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Cell wall formation By similarity. HAMAP-Rule MF_00047

Catalytic activity

ATP + 2 D-alanine = ADP + phosphate + D-alanyl-D-alanine. HAMAP-Rule MF_00047

Cofactor

Binds 2 magnesium or manganese ions per subunit By similarity.

Pathway

Cell wall biogenesis; peptidoglycan biosynthesis. HAMAP-Rule MF_00047

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00047.

Sequence similarities

Belongs to the D-alanine--D-alanine ligase family.

Contains 1 ATP-grasp domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 376376D-alanine--D-alanine ligase HAMAP-Rule MF_00047
PRO_1000189732

Regions

Domain150 – 358209ATP-grasp
Nucleotide binding183 – 23856ATP By similarity

Sites

Metal binding3111Magnesium or manganese 1 By similarity
Metal binding3251Magnesium or manganese 1 By similarity
Metal binding3251Magnesium or manganese 2 By similarity
Metal binding3271Magnesium or manganese 2 By similarity

Sequences

Sequence LengthMass (Da)Tools
B8I590 [UniParc].

Last modified March 3, 2009. Version 1.
Checksum: CC8C45AB08BB1613

FASTA37641,443
        10         20         30         40         50         60 
MSKKRVAIIF GGQSSEHEVS RVSAQSVIEN IDKQKYDVEI IGITKSGQWL KYDGPVEKIG 

        70         80         90        100        110        120 
NGDWEAIAQK KPVESLSQAG ISSSGITTVN SVRQIMPGNV KAPIDVIFPV LHGCNGEDGT 

       130        140        150        160        170        180 
IQGLFELAGI PYVGCGVLGS AVGMDKAYTK IIFEKEGLPQ GDYLVFNRKQ VYNRIEDVVA 

       190        200        210        220        230        240 
QIEGRLTYPC FVKPSNAGSS VGVNKASDRE SLVKALNIAA KNDRRILVEE FINGREIECA 

       250        260        270        280        290        300 
VLGNDNPVAS TVGEVVPCND FYDYEAKYQV GDSSKVVIPA PNLSKETVEK IREYAVRAFK 

       310        320        330        340        350        360 
CLDCAGLSRV DFFVHKETGE IYINEINTLP GFTQISMYPK LWAASGIPYS ELINKLIELA 

       370 
FERYEDTRRE YTNTLD 

« Hide

References

[1]"Complete sequence of Clostridium cellulolyticum H10."
US DOE Joint Genome Institute
Lucas S., Copeland A., Lapidus A., Glavina del Rio T., Dalin E., Tice H., Bruce D., Goodwin L., Pitluck S., Chertkov O., Saunders E., Brettin T., Detter J.C., Han C., Larimer F., Land M., Hauser L., Kyrpides N. expand/collapse author list , Ivanova N., Zhou J., Richardson P.
Submitted (JAN-2009) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 35319 / DSM 5812 / JCM 6584 / H10.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP001348 Genomic DNA. Translation: ACL74670.1.
RefSeqYP_002504650.1. NC_011898.1.

3D structure databases

ProteinModelPortalB8I590.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING394503.Ccel_0283.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaACL74670; ACL74670; Ccel_0283.
GeneID7309181.
KEGGcce:Ccel_0283.
PATRIC19431315. VBICloCel57783_0298.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG1181.
HOGENOMHOG000011593.
KOK01921.
OMAQIDVIFP.
OrthoDBEOG64BQ73.

Enzyme and pathway databases

BioCycCCEL394503:GJET-289-MONOMER.
UniPathwayUPA00219.

Family and domain databases

Gene3D3.30.1490.20. 1 hit.
3.30.470.20. 1 hit.
3.40.50.20. 1 hit.
HAMAPMF_00047. Dala_Dala_lig.
InterProIPR011761. ATP-grasp.
IPR013815. ATP_grasp_subdomain_1.
IPR013816. ATP_grasp_subdomain_2.
IPR000291. D-Ala_lig_Van_CS.
IPR005905. D_ala_D_ala.
IPR011095. Dala_Dala_lig_C.
IPR011127. Dala_Dala_lig_N.
IPR016185. PreATP-grasp_dom.
[Graphical view]
PANTHERPTHR23132. PTHR23132. 1 hit.
PfamPF07478. Dala_Dala_lig_C. 1 hit.
PF01820. Dala_Dala_lig_N. 1 hit.
[Graphical view]
SUPFAMSSF52440. SSF52440. 1 hit.
TIGRFAMsTIGR01205. D_ala_D_alaTIGR. 1 hit.
PROSITEPS50975. ATP_GRASP. 1 hit.
PS00843. DALA_DALA_LIGASE_1. 1 hit.
PS00844. DALA_DALA_LIGASE_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameDDL_CLOCE
AccessionPrimary (citable) accession number: B8I590
Entry history
Integrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: March 3, 2009
Last modified: May 14, 2014
This is version 38 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways