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B8I3S9 (FTHS_CLOCE) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 29. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Formate--tetrahydrofolate ligase

EC=6.3.4.3
Alternative name(s):
Formyltetrahydrofolate synthetase
Short name=FHS
Short name=FTHFS
Gene names
Name:fhs
Ordered Locus Names:Ccel_0018
OrganismClostridium cellulolyticum (strain ATCC 35319 / DSM 5812 / JCM 6584 / H10) [Complete proteome] [HAMAP]
Taxonomic identifier394503 [NCBI]
Taxonomic lineageBacteriaFirmicutesClostridiaClostridialesClostridiaceaeClostridium

Protein attributes

Sequence length556 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + formate + tetrahydrofolate = ADP + phosphate + 10-formyltetrahydrofolate. HAMAP-Rule MF_01543

Pathway

One-carbon metabolism; tetrahydrofolate interconversion. HAMAP-Rule MF_01543

Sequence similarities

Belongs to the formate--tetrahydrofolate ligase family.

Ontologies

Keywords
   Biological processOne-carbon metabolism
   LigandATP-binding
Nucleotide-binding
   Molecular functionLigase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processfolic acid-containing compound biosynthetic process

Inferred from electronic annotation. Source: InterPro

tetrahydrofolate interconversion

Inferred from electronic annotation. Source: UniProtKB-UniPathway

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

formate-tetrahydrofolate ligase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 556556Formate--tetrahydrofolate ligase HAMAP-Rule MF_01543
PRO_1000185252

Regions

Nucleotide binding65 – 728ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
B8I3S9 [UniParc].

Last modified March 3, 2009. Version 1.
Checksum: 2A6328B365FD75A4

FASTA55659,961
        10         20         30         40         50         60 
MQTDIQIAQR CKMHHIADIA KNLGIDTEDI EFYGNYKAKL SDKLWDKVKN KKDGKLVLVT 

        70         80         90        100        110        120 
AINPTPAGEG KTTTTVGLGQ AMARIGKNAV IALREPSMGP VMGIKGGAAG GGYAQVVPME 

       130        140        150        160        170        180 
DINLHFTGDM HAITAANNLL SAAIDNHLQQ GNMLNIDSRQ IVWKRCMDMN DRALRNVIVG 

       190        200        210        220        230        240 
LGGKINGVPR EDGFNITVAS EIMAILCLAL DIKDLKKRLG RIIIGYTYEG KPVTAHDLKV 

       250        260        270        280        290        300 
DGAMTLLLKD AIKPNLVQTL EGTPALMHGG PFANIAHGCN SISATKLALK LSDYVITEAG 

       310        320        330        340        350        360 
FGADLGAEKF FDIKCRFAGF KPDAVVLVAT IRALKYNGGV RKEDLKEENI DALSKGFANA 

       370        380        390        400        410        420 
EKHIENLKQF GVPVMVAINH FDTDTEAEIK LIQEKCSSLG VEVAFSDVFL KGGEGGIELA 

       430        440        450        460        470        480 
EKLVALTDST VSNFAPIYDE KLPIKEKVQQ IVSKIYGGRN VIYNAAAEKS IAKIEEMGLD 

       490        500        510        520        530        540 
RLPICMAKTQ YSLSDNPALL GRPQDFDVTV KEVRISAGAG FLVVLTGDIM TMPGLPKVPA 

       550 
AERIDINESG VITGLF 

« Hide

References

[1]"Complete sequence of Clostridium cellulolyticum H10."
US DOE Joint Genome Institute
Lucas S., Copeland A., Lapidus A., Glavina del Rio T., Dalin E., Tice H., Bruce D., Goodwin L., Pitluck S., Chertkov O., Saunders E., Brettin T., Detter J.C., Han C., Larimer F., Land M., Hauser L., Kyrpides N. expand/collapse author list , Ivanova N., Zhou J., Richardson P.
Submitted (JAN-2009) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 35319 / DSM 5812 / JCM 6584 / H10.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP001348 Genomic DNA. Translation: ACL74406.1.
RefSeqYP_002504386.1. NC_011898.1.

3D structure databases

ProteinModelPortalB8I3S9.
SMRB8I3S9. Positions 4-554.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING394503.Ccel_0018.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaACL74406; ACL74406; Ccel_0018.
GeneID7308941.
KEGGcce:Ccel_0018.
PATRIC19430753. VBICloCel57783_0020.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG2759.
HOGENOMHOG000040280.
KOK01938.
OMACGEIMTM.
OrthoDBEOG6PCPSP.
ProtClustDBCLSK2471997.

Enzyme and pathway databases

BioCycCCEL394503:GJET-20-MONOMER.
UniPathwayUPA00193.

Family and domain databases

Gene3D3.40.50.300. 2 hits.
HAMAPMF_01543. FTHFS.
InterProIPR000559. Formate_THF_ligase.
IPR020628. Formate_THF_ligase_CS.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamPF01268. FTHFS. 1 hit.
[Graphical view]
SUPFAMSSF52540. SSF52540. 1 hit.
PROSITEPS00721. FTHFS_1. 1 hit.
PS00722. FTHFS_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameFTHS_CLOCE
AccessionPrimary (citable) accession number: B8I3S9
Entry history
Integrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: March 3, 2009
Last modified: April 16, 2014
This is version 29 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways