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Protein

Hydroxyethylthiazole kinase

Gene

thiM

Organism
Clostridium cellulolyticum (strain ATCC 35319 / DSM 5812 / JCM 6584 / H10)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the phosphorylation of the hydroxyl group of 4-methyl-5-beta-hydroxyethylthiazole (THZ).UniRule annotation

Catalytic activityi

ATP + 4-methyl-5-(2-hydroxyethyl)thiazole = ADP + 4-methyl-5-(2-phosphonooxyethyl)thiazole.UniRule annotation

Cofactori

Mg2+UniRule annotation

Pathwayi: thiamine diphosphate biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes 4-methyl-5-(2-phosphoethyl)-thiazole from 5-(2-hydroxyethyl)-4-methylthiazole.UniRule annotation
Proteins known to be involved in this subpathway in this organism are:
  1. Hydroxyethylthiazole kinase (thiM)
This subpathway is part of the pathway thiamine diphosphate biosynthesis, which is itself part of Cofactor biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes 4-methyl-5-(2-phosphoethyl)-thiazole from 5-(2-hydroxyethyl)-4-methylthiazole, the pathway thiamine diphosphate biosynthesis and in Cofactor biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei47Substrate; via amide nitrogenUniRule annotation1
Binding sitei123ATPUniRule annotation1
Binding sitei172ATPUniRule annotation1
Binding sitei199Substrate; via amide nitrogenUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionKinase, Transferase
Biological processThiamine biosynthesis
LigandATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciCCEL394503:G1GUL-2029-MONOMER
UniPathwayiUPA00060; UER00139

Names & Taxonomyi

Protein namesi
Recommended name:
Hydroxyethylthiazole kinaseUniRule annotation (EC:2.7.1.50UniRule annotation)
Alternative name(s):
4-methyl-5-beta-hydroxyethylthiazole kinaseUniRule annotation
Short name:
TH kinaseUniRule annotation
Short name:
Thz kinaseUniRule annotation
Gene namesi
Name:thiMUniRule annotation
Ordered Locus Names:Ccel_1990
OrganismiClostridium cellulolyticum (strain ATCC 35319 / DSM 5812 / JCM 6584 / H10)
Taxonomic identifieri394503 [NCBI]
Taxonomic lineageiBacteriaFirmicutesClostridiaClostridialesRuminococcaceaeRuminiclostridium
Proteomesi
  • UP000001349 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003838481 – 273Hydroxyethylthiazole kinaseAdd BLAST273

Proteomic databases

PRIDEiB8I3J5

Interactioni

Protein-protein interaction databases

STRINGi394503.Ccel_1990

Structurei

3D structure databases

ProteinModelPortaliB8I3J5
SMRiB8I3J5
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the Thz kinase family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105C4U Bacteria
COG2145 LUCA
HOGENOMiHOG000114352
KOiK00878
OMAiKRPLVHN
OrthoDBiPOG091H004S

Family and domain databases

CDDicd01170 THZ_kinase, 1 hit
Gene3Di3.40.1190.20, 1 hit
HAMAPiMF_00228 Thz_kinase, 1 hit
InterProiView protein in InterPro
IPR000417 Hyethyz_kinase
IPR029056 Ribokinase-like
PANTHERiPTHR44423 PTHR44423, 1 hit
PfamiView protein in Pfam
PF02110 HK, 1 hit
PIRSFiPIRSF000513 Thz_kinase, 1 hit
PRINTSiPR01099 HYETHTZKNASE
SUPFAMiSSF53613 SSF53613, 1 hit
TIGRFAMsiTIGR00694 thiM, 1 hit

Sequencei

Sequence statusi: Complete.

B8I3J5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSEYTKQITR LISEVRSKKP LIHNITNYVT VNDCANVTLA IGASPIMADD
60 70 80 90 100
IDEAADITSI SSALVINIGT LNKRTIESMI LSGKKANEKG IPVIFDPVGA
110 120 130 140 150
GASALRNETT STILDKIKIS VLRGNLSEIS YIAGRNASTK GVDASEADIQ
160 170 180 190 200
SNDSIAVAKA AAVKLGCVVA VTGAVDVISD GKNVVTVLNG HKMLSNVTGT
210 220 230 240 250
GCMTTALVGS FCGAVKDYFK AAVAGVTVMG ISGEIAYEAA GHKGTGSYHI
260 270
AIIDAISRMD ENIFGEKARI NEI
Length:273
Mass (Da):28,408
Last modified:March 3, 2009 - v1
Checksum:iC6214012038A56D9
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001348 Genomic DNA Translation: ACL76338.1
RefSeqiWP_015925442.1, NC_011898.1

Genome annotation databases

EnsemblBacteriaiACL76338; ACL76338; Ccel_1990
KEGGicce:Ccel_1990

Similar proteinsi

Entry informationi

Entry nameiTHIM_CLOCE
AccessioniPrimary (citable) accession number: B8I3J5
Entry historyiIntegrated into UniProtKB/Swiss-Prot: September 22, 2009
Last sequence update: March 3, 2009
Last modified: May 23, 2018
This is version 55 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health