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B8I3J5 (THIM_CLOCE) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 34. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Hydroxyethylthiazole kinase

EC=2.7.1.50
Alternative name(s):
4-methyl-5-beta-hydroxyethylthiazole kinase
Short name=TH kinase
Short name=Thz kinase
Gene names
Name:thiM
Ordered Locus Names:Ccel_1990
OrganismClostridium cellulolyticum (strain ATCC 35319 / DSM 5812 / JCM 6584 / H10) [Complete proteome] [HAMAP]
Taxonomic identifier394503 [NCBI]
Taxonomic lineageBacteriaFirmicutesClostridiaClostridialesClostridiaceaeClostridium

Protein attributes

Sequence length273 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + 4-methyl-5-(2-hydroxyethyl)thiazole = ADP + 4-methyl-5-(2-phosphonooxyethyl)thiazole. HAMAP-Rule MF_00228

Cofactor

Magnesium By similarity. HAMAP-Rule MF_00228

Pathway

Cofactor biosynthesis; thiamine diphosphate biosynthesis; 4-methyl-5-(2-phosphoethyl)-thiazole from 5-(2-hydroxyethyl)-4-methylthiazole: step 1/1. HAMAP-Rule MF_00228

Sequence similarities

Belongs to the Thz kinase family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 273273Hydroxyethylthiazole kinase HAMAP-Rule MF_00228
PRO_0000383848

Sites

Binding site471Substrate; via amide nitrogen By similarity
Binding site1231ATP By similarity
Binding site1721ATP By similarity
Binding site1991Substrate; via amide nitrogen By similarity

Sequences

Sequence LengthMass (Da)Tools
B8I3J5 [UniParc].

Last modified March 3, 2009. Version 1.
Checksum: C6214012038A56D9

FASTA27328,408
        10         20         30         40         50         60 
MSEYTKQITR LISEVRSKKP LIHNITNYVT VNDCANVTLA IGASPIMADD IDEAADITSI 

        70         80         90        100        110        120 
SSALVINIGT LNKRTIESMI LSGKKANEKG IPVIFDPVGA GASALRNETT STILDKIKIS 

       130        140        150        160        170        180 
VLRGNLSEIS YIAGRNASTK GVDASEADIQ SNDSIAVAKA AAVKLGCVVA VTGAVDVISD 

       190        200        210        220        230        240 
GKNVVTVLNG HKMLSNVTGT GCMTTALVGS FCGAVKDYFK AAVAGVTVMG ISGEIAYEAA 

       250        260        270 
GHKGTGSYHI AIIDAISRMD ENIFGEKARI NEI 

« Hide

References

[1]"Complete sequence of Clostridium cellulolyticum H10."
US DOE Joint Genome Institute
Lucas S., Copeland A., Lapidus A., Glavina del Rio T., Dalin E., Tice H., Bruce D., Goodwin L., Pitluck S., Chertkov O., Saunders E., Brettin T., Detter J.C., Han C., Larimer F., Land M., Hauser L., Kyrpides N. expand/collapse author list , Ivanova N., Zhou J., Richardson P.
Submitted (JAN-2009) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 35319 / DSM 5812 / JCM 6584 / H10.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP001348 Genomic DNA. Translation: ACL76338.1.
RefSeqYP_002506318.1. NC_011898.1.

3D structure databases

ProteinModelPortalB8I3J5.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING394503.Ccel_1990.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaACL76338; ACL76338; Ccel_1990.
GeneID7310701.
KEGGcce:Ccel_1990.
PATRIC19434893. VBICloCel57783_2048.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG2145.
HOGENOMHOG000114352.
KOK00878.
OMALPLMATH.
OrthoDBEOG628F8M.
ProtClustDBPRK09355.

Enzyme and pathway databases

BioCycCCEL394503:GJET-2036-MONOMER.
UniPathwayUPA00060; UER00139.

Family and domain databases

HAMAPMF_00228. Thz_kinase.
InterProIPR000417. Hyethyz_kinase.
[Graphical view]
PfamPF02110. HK. 1 hit.
[Graphical view]
PIRSFPIRSF000513. Thz_kinase. 1 hit.
PRINTSPR01099. HYETHTZKNASE.
TIGRFAMsTIGR00694. thiM. 1 hit.
ProtoNetSearch...

Entry information

Entry nameTHIM_CLOCE
AccessionPrimary (citable) accession number: B8I3J5
Entry history
Integrated into UniProtKB/Swiss-Prot: September 22, 2009
Last sequence update: March 3, 2009
Last modified: February 19, 2014
This is version 34 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways