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Protein

NAD kinase

Gene

nadK

Organism
Clostridium cellulolyticum (strain ATCC 35319 / DSM 5812 / JCM 6584 / H10)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Involved in the regulation of the intracellular balance of NAD and NADP, and is a key enzyme in the biosynthesis of NADP. Catalyzes specifically the phosphorylation on 2'-hydroxyl of the adenosine moiety of NAD to yield NADP.UniRule annotation

Catalytic activityi

ATP + NAD+ = ADP + NADP+.UniRule annotation

Cofactori

a divalent metal cationUniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei67 – 671Proton acceptorUniRule annotation
Binding sitei72 – 721NADUniRule annotation
Binding sitei152 – 1521NADUniRule annotation
Binding sitei171 – 1711NADUniRule annotation
Binding sitei242 – 2421NADUniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi67 – 682NADUniRule annotation
Nucleotide bindingi141 – 1422NADUniRule annotation
Nucleotide bindingi182 – 1876NADUniRule annotation

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. metal ion binding Source: UniProtKB-HAMAP
  3. NAD+ kinase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. NAD metabolic process Source: InterPro
  2. NADP biosynthetic process Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Ligandi

ATP-binding, NAD, NADP, Nucleotide-binding

Enzyme and pathway databases

BioCyciCCEL394503:GJET-1943-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
NAD kinaseUniRule annotation (EC:2.7.1.23UniRule annotation)
Alternative name(s):
ATP-dependent NAD kinaseUniRule annotation
Gene namesi
Name:nadKUniRule annotation
Ordered Locus Names:Ccel_1898
OrganismiClostridium cellulolyticum (strain ATCC 35319 / DSM 5812 / JCM 6584 / H10)
Taxonomic identifieri394503 [NCBI]
Taxonomic lineageiBacteriaFirmicutesClostridiaClostridialesClostridiaceaeClostridium
ProteomesiUP000001349: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 286286NAD kinasePRO_1000133565Add
BLAST

Proteomic databases

PRIDEiB8I3A3.

Interactioni

Protein-protein interaction databases

STRINGi394503.Ccel_1898.

Family & Domainsi

Sequence similaritiesi

Belongs to the NAD kinase family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0061.
HOGENOMiHOG000227222.
KOiK00858.
OMAiGMVELRV.
OrthoDBiEOG6PZXDR.

Family and domain databases

Gene3Di2.60.200.30. 1 hit.
3.40.50.10330. 1 hit.
HAMAPiMF_00361. NAD_kinase.
InterProiIPR017438. ATP-NAD_kinase_dom_1.
IPR016064. ATP-NAD_kinase_PpnK-typ.
IPR017437. ATP-NAD_kinase_PpnK-typ_all-b.
IPR002504. PolyP/ATP_NADK.
[Graphical view]
PANTHERiPTHR20275. PTHR20275. 1 hit.
PfamiPF01513. NAD_kinase. 1 hit.
[Graphical view]
SUPFAMiSSF111331. SSF111331. 1 hit.

Sequencei

Sequence statusi: Complete.

B8I3A3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKKIGVITNR EKDKGLKYTN QLVESIEKHG GQAVLPTYDG SFQMDDIDNQ
60 70 80 90 100
VVEICNNCDM VICLGGDGTF LRTARTAYLY GLPMLGINLG SLGFLTDVEK
110 120 130 140 150
GEIDKAVENI LNNRFCLEDR IMLTSKLYKD GKLIARDVAI NDIVISRGGI
160 170 180 190 200
PRILHLSTYI DNNLVEMFPG DGIVVATPTG STAYSLSAGG PIVEPTSGLI
210 220 230 240 250
LITPICPHIL SSRALITSDM RKIKICVSQG FEHKATVTVD GQKNLEITGG
260 270 280
DYLEIEKANS TVKIIRVNSK NFFTVLRSKI YERKEE
Length:286
Mass (Da):31,463
Last modified:March 3, 2009 - v1
Checksum:i350DFB1B7702DAA7
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001348 Genomic DNA. Translation: ACL76246.1.
RefSeqiWP_015925351.1. NC_011898.1.
YP_002506226.1. NC_011898.1.

Genome annotation databases

EnsemblBacteriaiACL76246; ACL76246; Ccel_1898.
GeneIDi7310621.
KEGGicce:Ccel_1898.
PATRICi19434701. VBICloCel57783_1953.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001348 Genomic DNA. Translation: ACL76246.1.
RefSeqiWP_015925351.1. NC_011898.1.
YP_002506226.1. NC_011898.1.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi394503.Ccel_1898.

Proteomic databases

PRIDEiB8I3A3.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiACL76246; ACL76246; Ccel_1898.
GeneIDi7310621.
KEGGicce:Ccel_1898.
PATRICi19434701. VBICloCel57783_1953.

Phylogenomic databases

eggNOGiCOG0061.
HOGENOMiHOG000227222.
KOiK00858.
OMAiGMVELRV.
OrthoDBiEOG6PZXDR.

Enzyme and pathway databases

BioCyciCCEL394503:GJET-1943-MONOMER.

Family and domain databases

Gene3Di2.60.200.30. 1 hit.
3.40.50.10330. 1 hit.
HAMAPiMF_00361. NAD_kinase.
InterProiIPR017438. ATP-NAD_kinase_dom_1.
IPR016064. ATP-NAD_kinase_PpnK-typ.
IPR017437. ATP-NAD_kinase_PpnK-typ_all-b.
IPR002504. PolyP/ATP_NADK.
[Graphical view]
PANTHERiPTHR20275. PTHR20275. 1 hit.
PfamiPF01513. NAD_kinase. 1 hit.
[Graphical view]
SUPFAMiSSF111331. SSF111331. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 35319 / DSM 5812 / JCM 6584 / H10.

Entry informationi

Entry nameiNADK_CLOCE
AccessioniPrimary (citable) accession number: B8I3A3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: March 3, 2009
Last modified: March 4, 2015
This is version 42 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.