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B8I2R5

- PNP_CLOCE

UniProt

B8I2R5 - PNP_CLOCE

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Protein
Polyribonucleotide nucleotidyltransferase
Gene
pnp, Ccel_1707
Organism
Clostridium cellulolyticum (strain ATCC 35319 / DSM 5812 / JCM 6584 / H10)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Involved in mRNA degradation. Catalyzes the phosphorolysis of single-stranded polyribonucleotides processively in the 3'- to 5'-direction By similarity.UniRule annotation

Catalytic activityi

RNA(n+1) + phosphate = RNA(n) + a nucleoside diphosphate.UniRule annotation

Cofactori

Magnesium By similarity.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi486 – 4861Magnesium By similarity
Metal bindingi492 – 4921Magnesium By similarity

GO - Molecular functioni

  1. 3'-5'-exoribonuclease activity Source: InterPro
  2. RNA binding Source: UniProtKB-HAMAP
  3. magnesium ion binding Source: UniProtKB-HAMAP
  4. polyribonucleotide nucleotidyltransferase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. RNA processing Source: InterPro
  2. mRNA catabolic process Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Nucleotidyltransferase, Transferase

Keywords - Ligandi

Magnesium, Metal-binding, RNA-binding

Enzyme and pathway databases

BioCyciCCEL394503:GJET-1744-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Polyribonucleotide nucleotidyltransferase (EC:2.7.7.8)
Alternative name(s):
Polynucleotide phosphorylase
Short name:
PNPase
Gene namesi
Name:pnp
Ordered Locus Names:Ccel_1707
OrganismiClostridium cellulolyticum (strain ATCC 35319 / DSM 5812 / JCM 6584 / H10)
Taxonomic identifieri394503 [NCBI]
Taxonomic lineageiBacteriaFirmicutesClostridiaClostridialesClostridiaceaeClostridium
ProteomesiUP000001349: Chromosome

Subcellular locationi

Cytoplasm By similarity UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 703703Polyribonucleotide nucleotidyltransferaseUniRule annotation
PRO_1000185730Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi394503.Ccel_1707.

Structurei

3D structure databases

ProteinModelPortaliB8I2R5.
SMRiB8I2R5. Positions 619-693.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini554 – 61360KH
Add
BLAST
Domaini623 – 69169S1 motif
Add
BLAST

Sequence similaritiesi

Contains 1 KH domain.
Contains 1 S1 motif domain.

Phylogenomic databases

eggNOGiCOG1185.
HOGENOMiHOG000218326.
KOiK00962.
OMAiISMEDTH.
OrthoDBiEOG6WT8CC.

Family and domain databases

Gene3Di1.10.10.400. 1 hit.
2.40.50.140. 1 hit.
3.30.1370.10. 1 hit.
3.30.230.70. 2 hits.
HAMAPiMF_01595. PNPase.
InterProiIPR001247. ExoRNase_PH_dom1.
IPR015847. ExoRNase_PH_dom2.
IPR004087. KH_dom.
IPR004088. KH_dom_type_1.
IPR012340. NA-bd_OB-fold.
IPR012162. PNPase.
IPR027408. PNPase/RNase_PH_dom.
IPR015848. PNPase_PH_RNA-bd_bac/org-type.
IPR003029. Rbsml_prot_S1_RNA-bd_dom.
IPR020568. Ribosomal_S5_D2-typ_fold.
IPR022967. RNA-binding_domain_S1.
[Graphical view]
PANTHERiPTHR11252. PTHR11252. 1 hit.
PfamiPF00013. KH_1. 1 hit.
PF03726. PNPase. 1 hit.
PF01138. RNase_PH. 2 hits.
PF03725. RNase_PH_C. 1 hit.
PF00575. S1. 1 hit.
[Graphical view]
PIRSFiPIRSF005499. PNPase. 1 hit.
SMARTiSM00322. KH. 1 hit.
SM00316. S1. 1 hit.
[Graphical view]
SUPFAMiSSF46915. SSF46915. 1 hit.
SSF50249. SSF50249. 1 hit.
SSF54211. SSF54211. 2 hits.
SSF54791. SSF54791. 1 hit.
SSF55666. SSF55666. 2 hits.
TIGRFAMsiTIGR03591. polynuc_phos. 1 hit.
PROSITEiPS50084. KH_TYPE_1. 1 hit.
PS50126. S1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

B8I2R5-1 [UniParc]FASTAAdd to Basket

« Hide

MFKSFSMELA GRTLTVETGK LAQLANGSAL IRYGDTVIMS SATASAAPRD    50
GIDFFPLSVD YEERLYAVGK IPGGFIKREG KPSEKAILTS RVIDRPIRPL 100
FPKDLRNDVA VVNTVMSVEQ DNSPEIAAMI GASVAISISD IPFNGPIGGV 150
VLGLVDGEVI INPNEEQRAK SKMYVTLAGT KEKITMIEAG ADIVPDDVMF 200
DAIKKGHEEI KKICDFINGI VKEVGKAKFT YESADVPADL FEVVKEFAYD 250
KMRIAVLATD KQDRDAKVSQ LTEETQAALA EQFPEMESKI NDALYKLEKK 300
VVREYILKEG KRIDGRRLDE IRTLSAEVGI LPRTHGSGLF ERGQTQVLTT 350
VTLGAMGDVQ MLDGIDTEET KRYMHHYNFP GYSVGEAKTS RGPGRREIGH 400
GALAERSLVP VIPTETEFPY AFRLVSEVLM SNGSTSQGSV CGSTLALMDA 450
GVPIKAPVAG ISAGLVIDEE NPDNFVTFMD IQGIEDFFGD MDFKVAGTTE 500
GITAIQMDIK VDGLSYEIIR QAFELTRMGR LQIINDVILK AIPQPRKELS 550
EYAPKIITTN IDPEKIRDVI GPGGKMINKI IAETGVKIDI EEDGRVYILT 600
PDSAAAQKAL KIIQGIAKDI EPGEVYLGKV VRITTFGAFV EILPGKDGLV 650
HISKLDKKRV EKVEDVVSIG DEILVKVTEI DKQGRINLSR KDAMAEENTT 700
EEK 703
Length:703
Mass (Da):76,926
Last modified:March 3, 2009 - v1
Checksum:i6F42F528BA2F22ED
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP001348 Genomic DNA. Translation: ACL76058.1.
RefSeqiYP_002506038.1. NC_011898.1.

Genome annotation databases

EnsemblBacteriaiACL76058; ACL76058; Ccel_1707.
GeneIDi7310448.
KEGGicce:Ccel_1707.
PATRICi19434293. VBICloCel57783_1758.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP001348 Genomic DNA. Translation: ACL76058.1 .
RefSeqi YP_002506038.1. NC_011898.1.

3D structure databases

ProteinModelPortali B8I2R5.
SMRi B8I2R5. Positions 619-693.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 394503.Ccel_1707.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ACL76058 ; ACL76058 ; Ccel_1707 .
GeneIDi 7310448.
KEGGi cce:Ccel_1707.
PATRICi 19434293. VBICloCel57783_1758.

Phylogenomic databases

eggNOGi COG1185.
HOGENOMi HOG000218326.
KOi K00962.
OMAi ISMEDTH.
OrthoDBi EOG6WT8CC.

Enzyme and pathway databases

BioCyci CCEL394503:GJET-1744-MONOMER.

Family and domain databases

Gene3Di 1.10.10.400. 1 hit.
2.40.50.140. 1 hit.
3.30.1370.10. 1 hit.
3.30.230.70. 2 hits.
HAMAPi MF_01595. PNPase.
InterProi IPR001247. ExoRNase_PH_dom1.
IPR015847. ExoRNase_PH_dom2.
IPR004087. KH_dom.
IPR004088. KH_dom_type_1.
IPR012340. NA-bd_OB-fold.
IPR012162. PNPase.
IPR027408. PNPase/RNase_PH_dom.
IPR015848. PNPase_PH_RNA-bd_bac/org-type.
IPR003029. Rbsml_prot_S1_RNA-bd_dom.
IPR020568. Ribosomal_S5_D2-typ_fold.
IPR022967. RNA-binding_domain_S1.
[Graphical view ]
PANTHERi PTHR11252. PTHR11252. 1 hit.
Pfami PF00013. KH_1. 1 hit.
PF03726. PNPase. 1 hit.
PF01138. RNase_PH. 2 hits.
PF03725. RNase_PH_C. 1 hit.
PF00575. S1. 1 hit.
[Graphical view ]
PIRSFi PIRSF005499. PNPase. 1 hit.
SMARTi SM00322. KH. 1 hit.
SM00316. S1. 1 hit.
[Graphical view ]
SUPFAMi SSF46915. SSF46915. 1 hit.
SSF50249. SSF50249. 1 hit.
SSF54211. SSF54211. 2 hits.
SSF54791. SSF54791. 1 hit.
SSF55666. SSF55666. 2 hits.
TIGRFAMsi TIGR03591. polynuc_phos. 1 hit.
PROSITEi PS50084. KH_TYPE_1. 1 hit.
PS50126. S1. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 35319 / DSM 5812 / JCM 6584 / H10.

Entry informationi

Entry nameiPNP_CLOCE
AccessioniPrimary (citable) accession number: B8I2R5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: March 3, 2009
Last modified: May 14, 2014
This is version 40 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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