B8HYK1 (GSA_CYAP4) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Glutamate-1-semialdehyde 2,1-aminomutase
Short name=GSA
EC=5.4.3.8

Alternative name(s):
Glutamate-1-semialdehyde aminotransferase
Short name=GSA-AT

Gene names

Name:	hemL
Ordered Locus Names:	Cyan7425_4345

Organism

Cyanothece sp. (strain PCC 7425 / ATCC 29141) [Complete proteome] [HAMAP]

Taxonomic identifier

395961 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Cyanobacteria › Oscillatoriophycideae › Chroococcales › Cyanothece

Protein attributes

Sequence length	432 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	(S)-4-amino-5-oxopentanoate = 5-aminolevulinate. HAMAP-Rule MF_00375
Cofactor	Pyridoxal phosphate By similarity.
Pathway	Porphyrin metabolism; protoporphyrin-IX biosynthesis; 5-aminolevulinate from L-glutamyl-tRNA(Glu): step 2/2. HAMAP-Rule MF_00375 Porphyrin biosynthesis; chlorophyll biosynthesis. HAMAP-Rule MF_00375
Subunit structure	Homodimer By similarity.
Subcellular location	Cytoplasm Potential HAMAP-Rule MF_00375.
Sequence similarities	Belongs to the class-III pyridoxal-phosphate-dependent aminotransferase family. HemL subfamily.

Ontologies

Keywords
Biological process	Chlorophyll biosynthesis Porphyrin biosynthesis
Cellular component	Cytoplasm
Ligand	Pyridoxal phosphate
Molecular function	Isomerase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	chlorophyll biosynthetic process Inferred from electronic annotation. Source: HAMAP protoporphyrinogen IX biosynthetic process Inferred from electronic annotation. Source: UniProtKB-UniPathway
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	glutamate-1-semialdehyde 2,1-aminomutase activity Inferred from electronic annotation. Source: HAMAP pyridoxal phosphate binding Inferred from electronic annotation. Source: InterPro transaminase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 432	432	Glutamate-1-semialdehyde 2,1-aminomutase HAMAP-Rule MF_00375		PRO_1000201016
Amino acid modifications
	Modified residue	272	1	N6-(pyridoxal phosphate)lysine By similarity

Sequences

	Sequence		Length	Mass (Da)
	B8HYK1 [UniParc]. Last modified March 3, 2009. Version 1. Checksum: 7BD7E1DCF31E7195	FASTA	432	45,811
	10 20 30 40 50 60 MLTSTFNTTK SEEIFAAAQK LMPGGVNSPV RAFKSVGGNP IVFDRVEGAY VWDVDGNQYI 70 80 90 100 110 120 DYVGSWGPAI CGHAHPEVIK ALHAALDKGT SFGAPCLLEN VLAEMVIDGV PSIEMVRFVN 130 140 150 160 170 180 SGTEACMAIL RLMRAYTGRE KIIKFEGCYH GHADMFLVKA GSGVATLGLP DSPGVPKSVT 190 200 210 220 230 240 ANTLTAPFND LEAVKALFAD HPDQIAGVIL EPVVGNAGFI PPDGGFLAGL REITQEQGAL 250 260 270 280 290 300 LVFDEVMTGF RIAYGGAQEK FGVLPDLTTL GKIIGGGLPV GAYGGRREIM SLVAPAGPMY 310 320 330 340 350 360 QAGTLSGNPL AMTAGIKTLE LIRQTGTYEY LDQITAKLIN GLLTIAREAG HQVCGGHISG 370 380 390 400 410 420 MFGLFFTAGP VHNYEDAKKS DLAKFSAFHR GMLEQGVYLA PSQFEAGFTS LAHTEADIDR 430 TLEAARVVLN QL

References

[1]

"Novel metabolic attributes of the genus Cyanothece, comprising a group of unicellular nitrogen-fixing Cyanobacteria." Bandyopadhyay A., Elvitigala T., Welsh E., Stockel J., Liberton M., Min H., Sherman L.A., Pakrasi H.B. MBio 2:E214-E214(2011) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: PCC 7425 / ATCC 29141.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP001344 Genomic DNA. Translation: ACL46655.1.
RefSeq	YP_002485016.1. NC_011884.1.
3D structure databases
ProteinModelPortal	B8HYK1.
ModBase	Search...
Protein-protein interaction databases
STRING	395961.Cyan7425_4345.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	ACL46655; ACL46655; Cyan7425_4345.
GeneID	7290297.
KEGG	cyn:Cyan7425_4345.
PATRIC	21569685. VBICyaSp30657_4333.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0001.
HOGENOM	HOG000020210.
KO	K01845.
OMA	FGHADEE.
ProtClustDB	PRK00062.
Enzyme and pathway databases
BioCyc	CSP395961:GJDE-4190-MONOMER.
UniPathway	UPA00251; UER00317. UPA00668.
Family and domain databases
Gene3D	3.40.640.10. 1 hit. 3.90.1150.10. 2 hits.
HAMAP	MF_00375. HemL_aminotrans_3.
InterPro	IPR004639. 4pyrrol_synth_GluAld_NH2Trfase. IPR005814. Aminotrans_3. IPR015424. PyrdxlP-dep_Trfase. IPR015421. PyrdxlP-dep_Trfase_major_sub1. IPR015422. PyrdxlP-dep_Trfase_major_sub2. [Graphical view]
PANTHER	PTHR11986. PTHR11986. 1 hit. PTHR11986:SF5. PTHR11986:SF5. 1 hit.
Pfam	PF00202. Aminotran_3. 1 hit. [Graphical view]
SUPFAM	SSF53383. PyrdxlP-dep_Trfase_major. 1 hit.
TIGRFAMs	TIGR00713. hemL. 1 hit.
PROSITE	PS00600. AA_TRANSFER_CLASS_3. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GSA_CYAP4

Accession

Primary (citable) accession number: B8HYK1

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 28, 2009
Last sequence update:	March 3, 2009
Last modified:	May 1, 2013
This is version 35 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program