Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Glutamyl-tRNA(Gln) amidotransferase subunit A

Gene

gatA

Organism
Cyanothece sp. (strain PCC 7425 / ATCC 29141)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Allows the formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu-tRNA(Gln) in organisms which lack glutaminyl-tRNA synthetase. The reaction takes place in the presence of glutamine and ATP through an activated gamma-phospho-Glu-tRNA(Gln).UniRule annotation

Catalytic activityi

ATP + L-glutamyl-tRNA(Gln) + L-glutamine = ADP + phosphate + L-glutaminyl-tRNA(Gln) + L-glutamate.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei75Charge relay systemUniRule annotation1
Active sitei150Charge relay systemUniRule annotation1
Active sitei174Acyl-ester intermediateUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Glutamyl-tRNA(Gln) amidotransferase subunit AUniRule annotation (EC:6.3.5.7UniRule annotation)
Short name:
Glu-ADT subunit AUniRule annotation
Gene namesi
Name:gatAUniRule annotation
Ordered Locus Names:Cyan7425_2535
OrganismiCyanothece sp. (strain PCC 7425 / ATCC 29141)
Taxonomic identifieri395961 [NCBI]
Taxonomic lineageiBacteriaCyanobacteriaOscillatoriophycideaeOscillatorialesCyanothecaceaeCyanothece
Proteomesi
  • UP000002511 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_10001224771 – 482Glutamyl-tRNA(Gln) amidotransferase subunit AAdd BLAST482

Interactioni

Subunit structurei

Heterotrimer of A, B and C subunits.UniRule annotation

Protein-protein interaction databases

STRINGi395961.Cyan7425_2535.

Family & Domainsi

Sequence similaritiesi

Belongs to the amidase family. GatA subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105C3P. Bacteria.
COG0154. LUCA.
HOGENOMiHOG000116699.
KOiK02433.
OMAiKEYFGAG.
OrthoDBiPOG091H00O9.

Family and domain databases

Gene3Di3.90.1300.10. 1 hit.
HAMAPiMF_00120. GatA. 1 hit.
InterProiIPR000120. Amidase.
IPR020556. Amidase_CS.
IPR023631. Amidase_dom.
IPR004412. GatA.
[Graphical view]
PANTHERiPTHR11895. PTHR11895. 1 hit.
PfamiPF01425. Amidase. 1 hit.
[Graphical view]
SUPFAMiSSF75304. SSF75304. 1 hit.
TIGRFAMsiTIGR00132. gatA. 1 hit.
PROSITEiPS00571. AMIDASES. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

B8HY89-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MASIRELHQQ LVSKERSAKE ITQDALEKIQ QLEPKVHAFL TLTAEQALAQ
60 70 80 90 100
AERVDQQIAT GTEIGLLAGI PIAIKDNLCT KGIPTTCGSK ILQGFIPPYE
110 120 130 140 150
STVTSRLAAA GAVMVGKTNL DEFAMGSSTE NSAYQLTANP WDLQRVPGGS
160 170 180 190 200
SGGSAAAVAA GETLIALGSD TGGSIRQPAS FCGVVGLKPT YGLVSRYGLV
210 220 230 240 250
AYASSLDQIG PFATNVEDAA LLLGAIAGHD PQDSTSLNVP IPDYTQFLIP
260 270 280 290 300
DLKGKKIGII QETYGEGLDP QVEQVTHKAI QQLEELGAEV REISCPRFRY
310 320 330 340 350
GLPTYYIIAP SEASANLARY DGVKYGFRSP DPENLLSMYT RTRAEGFGPE
360 370 380 390 400
VKRRIMIGTY ALSAGYYDAY YLKAQKVRTL IKQDFEAAFE QVDVLVCPTA
410 420 430 440 450
PTTAFAAGAK TADPLSMYLS DLMTIPVNLA GLPGLSLPCG FDQQGLPIGL
460 470 480
QLIGNVLRED LVFQVAYAYE QATPWHDRHP QL
Length:482
Mass (Da):51,767
Last modified:March 3, 2009 - v1
Checksum:iEB3D4D75F7837CBB
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001344 Genomic DNA. Translation: ACL44892.1.
RefSeqiWP_012627965.1. NC_011884.1.

Genome annotation databases

EnsemblBacteriaiACL44892; ACL44892; Cyan7425_2535.
KEGGicyn:Cyan7425_2535.
PATRICi21566033. VBICyaSp30657_2530.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001344 Genomic DNA. Translation: ACL44892.1.
RefSeqiWP_012627965.1. NC_011884.1.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi395961.Cyan7425_2535.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiACL44892; ACL44892; Cyan7425_2535.
KEGGicyn:Cyan7425_2535.
PATRICi21566033. VBICyaSp30657_2530.

Phylogenomic databases

eggNOGiENOG4105C3P. Bacteria.
COG0154. LUCA.
HOGENOMiHOG000116699.
KOiK02433.
OMAiKEYFGAG.
OrthoDBiPOG091H00O9.

Family and domain databases

Gene3Di3.90.1300.10. 1 hit.
HAMAPiMF_00120. GatA. 1 hit.
InterProiIPR000120. Amidase.
IPR020556. Amidase_CS.
IPR023631. Amidase_dom.
IPR004412. GatA.
[Graphical view]
PANTHERiPTHR11895. PTHR11895. 1 hit.
PfamiPF01425. Amidase. 1 hit.
[Graphical view]
SUPFAMiSSF75304. SSF75304. 1 hit.
TIGRFAMsiTIGR00132. gatA. 1 hit.
PROSITEiPS00571. AMIDASES. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiGATA_CYAP4
AccessioniPrimary (citable) accession number: B8HY89
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: March 3, 2009
Last modified: November 2, 2016
This is version 46 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.