B8HWK8 (B8HWK8_CYAP4) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names

Recommended name:
1,4-alpha-glucan branching enzyme GlgB HAMAP MF_00685
EC=2.4.1.18 HAMAP MF_00685

Alternative name(s):
1,4-alpha-D-glucan:1,4-alpha-D-glucan 6-glucosyl-transferase HAMAP MF_00685
Alpha-(1->4)-glucan branching enzyme HAMAP MF_00685
Glycogen branching enzyme HAMAP MF_00685

Gene names

Name:	glgB HAMAP MF_00685
Ordered Locus Names:	Cyan7425_4120

Organism

Cyanothece sp. (strain PCC 7425 / ATCC 29141) [Complete proteome] [HAMAP] EMBL ACL46434.1

Taxonomic identifier

395961 [NCBI]

Taxonomic lineage

Bacteria › Cyanobacteria › Chroococcales › Cyanothece

Protein attributes

Sequence length	764 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the formation of the alpha-1,6-glucosidic linkages in glycogen by scission of a 1,4-alpha-linked oligosaccharide from growing alpha-1,4-glucan chains and the subsequent attachment of the oligosaccharide to the alpha-1,6 position By similarity. HAMAP MF_00685
Catalytic activity	Transfers a segment of a (1->4)-alpha-D-glucan chain to a primary hydroxy group in a similar glucan chain. HAMAP MF_00685 SAAS SAAS006407
Pathway	Glycan biosynthesis; glycogen biosynthesis. HAMAP MF_00685 SAAS SAAS006407
Subunit structure	Monomer By similarity. HAMAP MF_00685
Sequence similarities	Belongs to the glycosyl hydrolase 13 family. GlgB subfamily. HAMAP MF_00685

Ontologies

Keywords
Biological process	Carbohydrate metabolism Glycogen biosynthesis HAMAP MF_00685 SAAS SAAS006407 Glycogen metabolism HAMAP MF_00685
Molecular function	Glycosyltransferase HAMAP MF_00685 SAAS SAAS006407 Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glycogen biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	1,4-alpha-glucan branching enzyme activity Inferred from electronic annotation. Source: HAMAP cation binding Inferred from electronic annotation. Source: InterPro hydrolase activity, hydrolyzing O-glycosyl compounds Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Sites
	Active site	431	1	Nucleophile By similarity HAMAP MF_00685
	Active site	484	1	Proton donor By similarity HAMAP MF_00685

Sequences

	Sequence		Length	Mass (Da)
	B8HWK8 [UniParc]. Last modified March 3, 2009. Version 1. Checksum: E751E9D758C831F5	FASTA	764	88,452
	10 20 30 40 50 60 MTLSPDQVDQ IISNRHQDPF AILGPHLLEA DGTPTWVVRA YLPNAEAAWV LCPDERKEYS 70 80 90 100 110 120 MQTLHHPHFF ECTIATPELA NYQLRVKEGD HERVIYDPYA FPSPRLTEFD LHLYAEGNHH 130 140 150 160 170 180 RIYEKLGAHP TEVDGVRGVY FAVWAPNARN VSVIGDFNLW DGRQHQMRKG ITGIWELFIP 190 200 210 220 230 240 GLSPGEHYKY EIKNAAGHIY EKSDPFGFQQ EVRPKTASIV TDLNTYQWQD REWMERRRHT 250 260 270 280 290 300 DPLTQPVSVY EVHLGSWLHE SADNPPCSVD GQPLAAVPVA DLKPGARFLT YRELAERLIP 310 320 330 340 350 360 YVKELGFTHI ELLPIAEHPF DGSWGYQVVG YYACTSRYGT PQDFMYFVDQ CHQNGIGVII 370 380 390 400 410 420 DWVPGHFPRD GHGLAFFDGT HLYEHADPRK GEHKEWGTLV FNYSRHEVSN YLIANVLFWM 430 440 450 460 470 480 DKYHIDGIRV DAVASMLYLD YCRKPGEWVA NEYGGRENIE AANFLRHLNS LLFSYFPGVL 490 500 510 520 530 540 AIAEESTAWP MVSWPTYVGG LGFNLKWNMG WMHDILDYFH MDPWFRQFHQ NNVTFSIWYH 550 560 570 580 590 600 HSENFMLALS HDEVVHGKSN MLGKMPGDEW QKFANLRCLY GFMFAHPGKK TLFMSMEFGQ 610 620 630 640 650 660 WSEWNVWGDL EWHLLQFEPH QKLKQFIQTL NHLYRSQPAL HALDLGQDGF EWIDCSDNRH 670 680 690 700 710 720 SVVSFIRRSK DPEDFVIVVC NFTPQPHSHY RVGVPESGFY TELLNSDSRE YGGSNLGNLG 730 740 750 760 GKWTQEWAFH NRPYSLDLCL PPLATLVFKL DRDKSQANLG VYGS

References

[1]

"Complete sequence of chromosome Cyanothece sp. PCC 7425." Lucas S., Copeland A., Lapidus A., Glavina del Rio T., Dalin E., Tice H., Bruce D., Goodwin L., Pitluck S., Sims D., Meineke L., Brettin T., Detter J.C., Han C., Larimer F., Land M., Hauser L., Kyrpides N. , Ovchinnikova G., Liberton M., Stoeckel J., Banerjee A., Singh A., Page L., Sato H., Zhao L., Sherman L., Pakrasi H.B., Richardson P. Submitted (JAN-2009) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: PCC 7425 / ATCC 29141.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP001344 Genomic DNA. Translation: ACL46434.1.
RefSeq	YP_002484795.1. NC_011884.1.
3D structure databases
ProteinModelPortal	B8HWK8.
ModBase	Search...
Protein-protein interaction databases
STRING	B8HWK8.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	7290070.
GenomeReviews	Gene locus Cyan7425_4120 in contig CP001344_GR.
KEGG	cyn:Cyan7425_4120.
PATRIC	21569223. VBICyaSp30657_4103.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG287139.
OMA	WEIFAPD.
PhylomeDB	B8HWK8.
ProtClustDB	PRK05402.
Family and domain databases
HAMAP	MF_00685. GlgB. [Tree]
InterPro	IPR006407. 1-4-A-glucan_branch_enz. IPR006048. A-amylase_b_C. IPR015902. Alpha_amylase. IPR013780. Glyco_hydro_13_b. IPR006047. Glyco_hydro_13_cat_dom. IPR004193. Glyco_hydro_13_N. IPR013781. Glyco_hydro_subgr_catalytic. IPR017853. Glycoside_hydrolase_SF. IPR013783. Ig-like_fold. IPR014756. Ig_E-set. [Graphical view]
Gene3D	G3DSA:2.60.40.1180. Glyco_hydro_13_b. 1 hit. G3DSA:3.20.20.80. Glyco_hydro_cat. 1 hit. G3DSA:2.60.40.10. Ig-like_fold. 2 hits.
KO	K00700.
PANTHER	PTHR10357. Alpha_amylase. 1 hit. PTHR10357:SF13. PTHR10357:SF13. 1 hit.
Pfam	PF00128. Alpha-amylase. 1 hit. PF02806. Alpha-amylase_C. 1 hit. PF02922. CBM_48. 1 hit. [Graphical view]
PIRSF	PIRSF000463. GlgB. 1 hit.
SUPFAM	SSF51445. Glyco_hydro_cat. 1 hit. SSF81296. Ig_E-set. 1 hit.
TIGRFAMs	TIGR01515. Branching_enzym. 1 hit.
ProtoNet	Search...

Entry information

Entry name

B8HWK8_CYAP4

Accession

Primary (citable) accession number: B8HWK8

Entry history

Integrated into UniProtKB/TrEMBL:	March 3, 2009
Last sequence update:	March 3, 2009
Last modified:	December 14, 2011
This is version 25 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)