B8HU41 (ISPE_CYAP4) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
4-diphosphocytidyl-2-C-methyl-D-erythritol kinase
Short name=CMK
EC=2.7.1.148

Alternative name(s):
4-(cytidine-5'-diphospho)-2-C-methyl-D-erythritol kinase

Gene names

Name:	ispE
Ordered Locus Names:	Cyan7425_0570

Organism

Cyanothece sp. (strain PCC 7425 / ATCC 29141) [Complete proteome] [HAMAP]

Taxonomic identifier

395961 [NCBI]

Taxonomic lineage

Bacteria › Cyanobacteria › Chroococcales › Cyanothece

Protein attributes

Sequence length	324 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the phosphorylation of the position 2 hydroxy group of 4-diphosphocytidyl-2C-methyl-D-erythritol By similarity. HAMAP MF_00061
Catalytic activity	ATP + 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol = ADP + 2-phospho-4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol. HAMAP MF_00061
Pathway	Isoprenoid biosynthesis; isopentenyl diphosphate biosynthesis via DXP pathway; isopentenyl diphosphate from 1-deoxy-D-xylulose 5-phosphate: step 3/6. HAMAP MF_00061
Sequence similarities	Belongs to the GHMP kinase family. IspE subfamily.

Ontologies

Keywords
Biological process	Isoprene biosynthesis
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	terpenoid biosynthetic process Inferred from electronic annotation. Source: InterPro
Molecular function	4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol kinase activity Inferred from electronic annotation. Source: EC ATP binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 324	324	4-diphosphocytidyl-2-C-methyl-D-erythritol kinase HAMAP MF_00061		PRO_1000190683
Regions
	Nucleotide binding	108 – 118	11	ATP Potential
Sites
	Active site	11	1	By similarity
	Active site	150	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	B8HU41 [UniParc]. Last modified March 3, 2009. Version 1. Checksum: 903F8A02CA77F5EB	FASTA	324	35,268
	10 20 30 40 50 60 MRAYTLIAPA KINLFLQIIG DHLQQDQPTG YHNLVMVLQS VSLSDELQLR PLSGEARSLR 70 80 90 100 110 120 LDPPILLHCD HPQVPLDQTN LVYRAAALMW QKFPGQAGVE ITLHKRIPIG AGLAGGSTDA 130 140 150 160 170 180 AAVLVGLNLM WELGLTQLEL QELGSQLGAD VPFCIRGGTS LAVGRGDQLS PLPDLEGIYV 190 200 210 220 230 240 VLGKYHDLSV STPWAYQTYR QQFQASYAQT LEEQEQRRQQ GGSGALLKAI AHRDGGQIGQ 250 260 270 280 290 300 LLHNDLEKVV LPAYPRVEYL RQQFANQSPL GTMMSGSGPT VFALADSAAA AEEIYAGVRG 310 320 AIADPYLDLW ICQLCNQGIQ VQPL

References

[1]

"Complete sequence of chromosome Cyanothece sp. PCC 7425." US DOE Joint Genome Institute Lucas S., Copeland A., Lapidus A., Glavina del Rio T., Dalin E., Tice H., Bruce D., Goodwin L., Pitluck S., Sims D., Meineke L., Brettin T., Detter J.C., Han C., Larimer F., Land M., Hauser L., Kyrpides N. , Ovchinnikova G., Liberton M., Stoeckel J., Banerjee A., Singh A., Page L., Sato H., Zhao L., Sherman L., Pakrasi H.B., Richardson P. Submitted (JAN-2009) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: PCC 7425 / ATCC 29141.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP001344 Genomic DNA. Translation: ACL42961.1.
RefSeq	YP_002481322.1. NC_011884.1.
3D structure databases
ProteinModelPortal	B8HU41.
ModBase	Search...
Protein-protein interaction databases
STRING	B8HU41.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	7286479.
GenomeReviews	Gene locus Cyan7425_0570 in contig CP001344_GR.
KEGG	cyn:Cyan7425_0570.
PATRIC	21562045. VBICyaSp30657_0557.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG734593.
OMA	QLCFGRG.
PhylomeDB	B8HU41.
ProtClustDB	PRK02534.
Family and domain databases
HAMAP	MF_00061. IspE. [Tree]
InterPro	IPR006204. GHMP_kinase. IPR013750. GHMP_kinase_C. IPR004424. IspE. IPR020568. Ribosomal_S5_D2-typ_fold. IPR014721. Ribosomal_S5_D2-typ_fold_subgr. [Graphical view]
Gene3D	G3DSA:3.30.230.10. Ribosomal_S5_D2-type_fold. 1 hit.
KO	K00919.
PANTHER	PTHR20861:SF2. IspE. 1 hit.
Pfam	PF08544. GHMP_kinases_C. 1 hit. PF00288. GHMP_kinases_N. 1 hit. [Graphical view]
PIRSF	PIRSF010376. IspE. 1 hit.
SUPFAM	SSF54211. Ribosomal_S5_D2-typ_fold. 1 hit.
TIGRFAMs	TIGR00154. IspE. 1 hit.
ProtoNet	Search...

Entry information

Entry name

ISPE_CYAP4

Accession

Primary (citable) accession number: B8HU41

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 28, 2009
Last sequence update:	March 3, 2009
Last modified:	January 25, 2012
This is version 22 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program