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B8HU20

- DAPF_CYAP4

UniProt

B8HU20 - DAPF_CYAP4

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Protein
Diaminopimelate epimerase
Gene
dapF, Cyan7425_0549
Organism
Cyanothece sp. (strain PCC 7425 / ATCC 29141)
Status
Reviewed - Annotation score: 3 out of 5 - Experimental evidence at protein leveli

Functioni

Catalyzes the stereoinversion of LL-2,6-diaminoheptanedioate (L,L-DAP) to meso-diaminoheptanedioate (meso-DAP), a precursor of L-lysine and an essential component of the bacterial peptidoglycan By similarity.UniRule annotation

Catalytic activityi

LL-2,6-diaminoheptanedioate = meso-diaminoheptanedioate.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei13 – 131Substrate By similarity
Binding sitei47 – 471Substrate By similarity
Binding sitei66 – 661Substrate By similarity
Active sitei75 – 751Proton donor/acceptor By similarity
Binding sitei165 – 1651Substrate By similarity
Sitei167 – 1671Important for catalytic activity By similarity
Binding sitei198 – 1981Substrate By similarity
Sitei216 – 2161Important for catalytic activity By similarity
Active sitei225 – 2251Proton donor/acceptor By similarity

GO - Molecular functioni

  1. diaminopimelate epimerase activity Source: UniProtKB-HAMAP
Complete GO annotation...

GO - Biological processi

  1. lysine biosynthetic process via diaminopimelate Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Isomerase

Keywords - Biological processi

Amino-acid biosynthesis, Lysine biosynthesis

Enzyme and pathway databases

BioCyciCSP395961:GJDE-356-MONOMER.
UniPathwayiUPA00034; UER00025.

Names & Taxonomyi

Protein namesi
Recommended name:
Diaminopimelate epimerase (EC:5.1.1.7)
Short name:
DAP epimerase
Gene namesi
Name:dapF
Ordered Locus Names:Cyan7425_0549
OrganismiCyanothece sp. (strain PCC 7425 / ATCC 29141)
Taxonomic identifieri395961 [NCBI]
Taxonomic lineageiBacteriaCyanobacteriaOscillatoriophycideaeChroococcalesCyanothece
ProteomesiUP000002511: Chromosome

Subcellular locationi

Cytoplasm By similarity UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 280280Diaminopimelate epimeraseUniRule annotation
PRO_1000124409Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi75 ↔ 225UniRule annotation

Keywords - PTMi

Disulfide bond

Interactioni

Protein-protein interaction databases

STRINGi395961.Cyan7425_0549.

Structurei

3D structure databases

ProteinModelPortaliB8HU20.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni10 – 112Substrate binding By similarity
Regioni75 – 773Substrate binding By similarity
Regioni216 – 2172Substrate binding By similarity
Regioni226 – 2272Substrate binding By similarity

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0253.
HOGENOMiHOG000220467.
KOiK01778.
OMAiLIVEPPY.
OrthoDBiEOG6ND0M5.

Family and domain databases

HAMAPiMF_00197. DAP_epimerase.
InterProiIPR018510. DAP_epimerase_AS.
IPR001653. DAP_epimerase_DapF.
[Graphical view]
PANTHERiPTHR31689. PTHR31689. 1 hit.
PfamiPF01678. DAP_epimerase. 2 hits.
[Graphical view]
TIGRFAMsiTIGR00652. DapF. 1 hit.
PROSITEiPS01326. DAP_EPIMERASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

B8HU20-1 [UniParc]FASTAAdd to Basket

« Hide

MSLQFTKYQG LGNDFILIDN RNEATPLLTP EQAVDICDRH FGVGADGVIF    50
LLPATGETDY TMRIFNSDGS EPEMCGNGIR CLARFAADLE GHALTATPRY 100
RIHTLAGTIT PQVRSDGQVT VDMGQPRLLA GEIPTTLKPA AEKVIAQPLQ 150
VGGRSWSVTC VSMGNPHCIT FVENVAAIDL HTLGPQFEHH PAFPQRINTE 200
FIEVIRPDYL KMRVWERGAG MTLACGTGAC AALVAAVLND LSEPRATVEL 250
PGGPLEIEWS APGQAVLMTG PAEQVFQGVL 280
Length:280
Mass (Da):30,164
Last modified:March 3, 2009 - v1
Checksum:iA8333703E11C34B9
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP001344 Genomic DNA. Translation: ACL42940.1.
RefSeqiWP_012626041.1. NC_011884.1.
YP_002481301.1. NC_011884.1.

Genome annotation databases

EnsemblBacteriaiACL42940; ACL42940; Cyan7425_0549.
GeneIDi7286458.
KEGGicyn:Cyan7425_0549.
PATRICi21562001. VBICyaSp30657_0535.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP001344 Genomic DNA. Translation: ACL42940.1 .
RefSeqi WP_012626041.1. NC_011884.1.
YP_002481301.1. NC_011884.1.

3D structure databases

ProteinModelPortali B8HU20.
ModBasei Search...

Protein-protein interaction databases

STRINGi 395961.Cyan7425_0549.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ACL42940 ; ACL42940 ; Cyan7425_0549 .
GeneIDi 7286458.
KEGGi cyn:Cyan7425_0549.
PATRICi 21562001. VBICyaSp30657_0535.

Phylogenomic databases

eggNOGi COG0253.
HOGENOMi HOG000220467.
KOi K01778.
OMAi LIVEPPY.
OrthoDBi EOG6ND0M5.

Enzyme and pathway databases

UniPathwayi UPA00034 ; UER00025 .
BioCyci CSP395961:GJDE-356-MONOMER.

Family and domain databases

HAMAPi MF_00197. DAP_epimerase.
InterProi IPR018510. DAP_epimerase_AS.
IPR001653. DAP_epimerase_DapF.
[Graphical view ]
PANTHERi PTHR31689. PTHR31689. 1 hit.
Pfami PF01678. DAP_epimerase. 2 hits.
[Graphical view ]
TIGRFAMsi TIGR00652. DapF. 1 hit.
PROSITEi PS01326. DAP_EPIMERASE. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Novel metabolic attributes of the genus Cyanothece, comprising a group of unicellular nitrogen-fixing Cyanobacteria."
    Bandyopadhyay A., Elvitigala T., Welsh E., Stockel J., Liberton M., Min H., Sherman L.A., Pakrasi H.B.
    MBio 2:E214-E214(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: PCC 7425 / ATCC 29141.

Entry informationi

Entry nameiDAPF_CYAP4
AccessioniPrimary (citable) accession number: B8HU20
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: March 3, 2009
Last modified: September 3, 2014
This is version 39 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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