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B8HU20

- DAPF_CYAP4

UniProt

B8HU20 - DAPF_CYAP4

Protein

Diaminopimelate epimerase

Gene

dapF

Organism
Cyanothece sp. (strain PCC 7425 / ATCC 29141)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 40 (01 Oct 2014)
      Sequence version 1 (03 Mar 2009)
      Previous versions | rss
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    Functioni

    Catalyzes the stereoinversion of LL-2,6-diaminoheptanedioate (L,L-DAP) to meso-diaminoheptanedioate (meso-DAP), a precursor of L-lysine and an essential component of the bacterial peptidoglycan.UniRule annotation

    Catalytic activityi

    LL-2,6-diaminoheptanedioate = meso-diaminoheptanedioate.UniRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei13 – 131SubstrateUniRule annotation
    Binding sitei47 – 471SubstrateUniRule annotation
    Binding sitei66 – 661SubstrateUniRule annotation
    Active sitei75 – 751Proton donor/acceptorUniRule annotation
    Binding sitei165 – 1651SubstrateUniRule annotation
    Sitei167 – 1671Important for catalytic activityUniRule annotation
    Binding sitei198 – 1981SubstrateUniRule annotation
    Sitei216 – 2161Important for catalytic activityUniRule annotation
    Active sitei225 – 2251Proton donor/acceptorUniRule annotation

    GO - Molecular functioni

    1. diaminopimelate epimerase activity Source: UniProtKB-HAMAP

    GO - Biological processi

    1. lysine biosynthetic process via diaminopimelate Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Isomerase

    Keywords - Biological processi

    Amino-acid biosynthesis, Lysine biosynthesis

    Enzyme and pathway databases

    BioCyciCSP395961:GJDE-356-MONOMER.
    UniPathwayiUPA00034; UER00025.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Diaminopimelate epimeraseUniRule annotation (EC:5.1.1.7UniRule annotation)
    Short name:
    DAP epimeraseUniRule annotation
    Gene namesi
    Name:dapFUniRule annotation
    Ordered Locus Names:Cyan7425_0549
    OrganismiCyanothece sp. (strain PCC 7425 / ATCC 29141)
    Taxonomic identifieri395961 [NCBI]
    Taxonomic lineageiBacteriaCyanobacteriaOscillatoriophycideaeChroococcalesCyanothece
    ProteomesiUP000002511: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 280280Diaminopimelate epimerasePRO_1000124409Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Disulfide bondi75 ↔ 225

    Keywords - PTMi

    Disulfide bond

    Interactioni

    Protein-protein interaction databases

    STRINGi395961.Cyan7425_0549.

    Structurei

    3D structure databases

    ProteinModelPortaliB8HU20.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni10 – 112Substrate bindingUniRule annotation
    Regioni75 – 773Substrate bindingUniRule annotation
    Regioni216 – 2172Substrate bindingUniRule annotation
    Regioni226 – 2272Substrate bindingUniRule annotation

    Sequence similaritiesi

    Belongs to the diaminopimelate epimerase family.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0253.
    HOGENOMiHOG000220467.
    KOiK01778.
    OMAiLIVEPPY.
    OrthoDBiEOG6ND0M5.

    Family and domain databases

    HAMAPiMF_00197. DAP_epimerase.
    InterProiIPR018510. DAP_epimerase_AS.
    IPR001653. DAP_epimerase_DapF.
    [Graphical view]
    PANTHERiPTHR31689. PTHR31689. 1 hit.
    PfamiPF01678. DAP_epimerase. 2 hits.
    [Graphical view]
    TIGRFAMsiTIGR00652. DapF. 1 hit.
    PROSITEiPS01326. DAP_EPIMERASE. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    B8HU20-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSLQFTKYQG LGNDFILIDN RNEATPLLTP EQAVDICDRH FGVGADGVIF    50
    LLPATGETDY TMRIFNSDGS EPEMCGNGIR CLARFAADLE GHALTATPRY 100
    RIHTLAGTIT PQVRSDGQVT VDMGQPRLLA GEIPTTLKPA AEKVIAQPLQ 150
    VGGRSWSVTC VSMGNPHCIT FVENVAAIDL HTLGPQFEHH PAFPQRINTE 200
    FIEVIRPDYL KMRVWERGAG MTLACGTGAC AALVAAVLND LSEPRATVEL 250
    PGGPLEIEWS APGQAVLMTG PAEQVFQGVL 280
    Length:280
    Mass (Da):30,164
    Last modified:March 3, 2009 - v1
    Checksum:iA8333703E11C34B9
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP001344 Genomic DNA. Translation: ACL42940.1.
    RefSeqiWP_012626041.1. NC_011884.1.
    YP_002481301.1. NC_011884.1.

    Genome annotation databases

    EnsemblBacteriaiACL42940; ACL42940; Cyan7425_0549.
    GeneIDi7286458.
    KEGGicyn:Cyan7425_0549.
    PATRICi21562001. VBICyaSp30657_0535.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP001344 Genomic DNA. Translation: ACL42940.1 .
    RefSeqi WP_012626041.1. NC_011884.1.
    YP_002481301.1. NC_011884.1.

    3D structure databases

    ProteinModelPortali B8HU20.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 395961.Cyan7425_0549.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai ACL42940 ; ACL42940 ; Cyan7425_0549 .
    GeneIDi 7286458.
    KEGGi cyn:Cyan7425_0549.
    PATRICi 21562001. VBICyaSp30657_0535.

    Phylogenomic databases

    eggNOGi COG0253.
    HOGENOMi HOG000220467.
    KOi K01778.
    OMAi LIVEPPY.
    OrthoDBi EOG6ND0M5.

    Enzyme and pathway databases

    UniPathwayi UPA00034 ; UER00025 .
    BioCyci CSP395961:GJDE-356-MONOMER.

    Family and domain databases

    HAMAPi MF_00197. DAP_epimerase.
    InterProi IPR018510. DAP_epimerase_AS.
    IPR001653. DAP_epimerase_DapF.
    [Graphical view ]
    PANTHERi PTHR31689. PTHR31689. 1 hit.
    Pfami PF01678. DAP_epimerase. 2 hits.
    [Graphical view ]
    TIGRFAMsi TIGR00652. DapF. 1 hit.
    PROSITEi PS01326. DAP_EPIMERASE. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Novel metabolic attributes of the genus Cyanothece, comprising a group of unicellular nitrogen-fixing Cyanobacteria."
      Bandyopadhyay A., Elvitigala T., Welsh E., Stockel J., Liberton M., Min H., Sherman L.A., Pakrasi H.B.
      MBio 2:E214-E214(2011) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: PCC 7425 / ATCC 29141.

    Entry informationi

    Entry nameiDAPF_CYAP4
    AccessioniPrimary (citable) accession number: B8HU20
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 28, 2009
    Last sequence update: March 3, 2009
    Last modified: October 1, 2014
    This is version 40 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3