B8HRT5 (DNAA_CYAP4) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Chromosomal replication initiator protein DnaA

Gene names

Name:	dnaA
Ordered Locus Names:	Cyan7425_0198

Organism

Cyanothece sp. (strain PCC 7425 / ATCC 29141) [Complete proteome] [HAMAP]

Taxonomic identifier

395961 [NCBI]

Taxonomic lineage

Bacteria › Cyanobacteria › Chroococcales › Cyanothece

Protein attributes

Sequence length	460 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Plays an important role in the initiation and regulation of chromosomal replication. Binds to the origin of replication; it binds specifically double-stranded DNA at a 9 bp consensus (dnaA box): 5'-TTATC[CA]A[CA]A-3'. DnaA binds to ATP and to acidic phospholipids By similarity. HAMAP MF_00377
Subcellular location	Cytoplasm By similarity HAMAP MF_00377.
Sequence similarities	Belongs to the DnaA family.

Ontologies

Keywords
Biological process	DNA replication
Cellular component	Cytoplasm
Ligand	ATP-binding DNA-binding Nucleotide-binding
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	DNA-dependent DNA replication initiation Inferred from electronic annotation. Source: InterPro regulation of DNA replication Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW DNA replication origin binding Inferred from electronic annotation. Source: InterPro nucleoside-triphosphatase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 460	460	Chromosomal replication initiator protein DnaA HAMAP MF_00377		PRO_1000189794
Regions
	Nucleotide binding	160 – 167	8	ATP Potential

Sequences

	Sequence		Length	Mass (Da)
	B8HRT5 [UniParc]. Last modified March 3, 2009. Version 1. Checksum: 3410CEC1DF011BA7	FASTA	460	51,998
	10 20 30 40 50 60 MDISLESLWN QILERLQLQL SRPTFETWIK TATAEAFTED RLTIRTPNPF ARNWIQKYYL 70 80 90 100 110 120 KTIADVAHDI LGHPVDVQLV IAEGEETPSS EETDLGFAFP PLFRNPSPAP APQRERMSDL 130 140 150 160 170 180 NPKYVFSRYV VGPNNRMAHA ACLAVAESPG REFNPLFLCG GVGLGKTHLM QAIGHYRLEI 190 200 210 220 230 240 CPDSKIFYVS TEQFTNDLIA AIRKDSMQSF REHYRAVDVM LVDDIQFIEG KEYTQEEFFH 250 260 270 280 290 300 TFNTLHEAGK QVVLASDRPP SQIPRLQERL CSRFSMGLIA DIQPPDLETR MAILQKKAEY 310 320 330 340 350 360 ENIRLPREVI EYIASSYTSN IRELEGALIR AVAYISISGL PMTVENIAPV LTPTTAKLEA 370 380 390 400 410 420 SPQTILLAVS DTFGIPIEDL KGNSRRREIS VARQVGMYLM RQHTGLSLPK IGEEFGGKDH 430 440 450 460 TTVLYSCEKV ADLQRTDPEI AQTLRQLSDR INLASRQTEN

References

[1]

"Complete sequence of chromosome Cyanothece sp. PCC 7425." US DOE Joint Genome Institute Lucas S., Copeland A., Lapidus A., Glavina del Rio T., Dalin E., Tice H., Bruce D., Goodwin L., Pitluck S., Sims D., Meineke L., Brettin T., Detter J.C., Han C., Larimer F., Land M., Hauser L., Kyrpides N. , Ovchinnikova G., Liberton M., Stoeckel J., Banerjee A., Singh A., Page L., Sato H., Zhao L., Sherman L., Pakrasi H.B., Richardson P. Submitted (JAN-2009) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: PCC 7425 / ATCC 29141.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP001344 Genomic DNA. Translation: ACL42594.1.
RefSeq	YP_002480955.1. NC_011884.1.
3D structure databases
ProteinModelPortal	B8HRT5.
ModBase	Search...
Protein-protein interaction databases
STRING	B8HRT5.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	7291224.
GenomeReviews	Gene locus Cyan7425_0198 in contig CP001344_GR.
KEGG	cyn:Cyan7425_0198.
PATRIC	21561299. VBICyaSp30657_0188.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG695666.
OMA	CHTLNAL.
PhylomeDB	B8HRT5.
ProtClustDB	PRK00149.
Family and domain databases
HAMAP	MF_00377. DnaA_bact. [Tree]
InterPro	IPR003593. ATPase_AAA+_core. IPR001957. Chromosome_initiator_DnaA. IPR020591. Chromosome_initiator_DnaA-like. IPR018312. Chromosome_initiator_DnaA_CS. IPR013159. DnaA_C. IPR013317. DnaA_Hda. IPR010921. Trp_repressor/repl_initiator. [Graphical view]
Gene3D	G3DSA:1.10.1750.10. DnaA_C. 1 hit.
KO	K02313.
Pfam	PF00308. Bac_DnaA. 1 hit. PF08299. Bac_DnaA_C. 1 hit. [Graphical view]
PRINTS	PR00051. DNAA.
SMART	SM00382. AAA. 1 hit. SM00760. Bac_DnaA_C. 1 hit. [Graphical view]
SUPFAM	SSF48295. Trp_repress_rep. 1 hit.
TIGRFAMs	TIGR00362. DnaA. 1 hit.
PROSITE	PS01008. DNAA. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DNAA_CYAP4

Accession

Primary (citable) accession number: B8HRT5

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 28, 2009
Last sequence update:	March 3, 2009
Last modified:	January 25, 2012
This is version 21 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program