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B8HR59 (GLYA_CYAP4) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 23. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Pyridoxal-phosphate-dependent serine hydroxymethyltransferase
Short name=SHMT
Short name=Serine methylase
EC=2.1.2.1
Gene names
Name:glyA
Ordered Locus Names:Cyan7425_1678
OrganismCyanothece sp. (strain PCC 7425 / ATCC 29141) [Complete proteome] [HAMAP]
Taxonomic identifier395961 [NCBI]
Taxonomic lineageBacteria › Cyanobacteria › Chroococcales › Cyanothece

Protein attributes

Sequence length426 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the reversible interconversion of serine and glycine with tetrahydrofolate serving as the one-carbon carrier By similarity.

Catalytic activity

5,10-methylenetetrahydrofolate + glycine + H2O = tetrahydrofolate + L-serine. HAMAP MF_00051

Cofactor

Pyridoxal phosphate By similarity. HAMAP MF_00051

Pathway

One-carbon metabolism; tetrahydrofolate interconversion. HAMAP MF_00051

Amino-acid biosynthesis; glycine biosynthesis; glycine from L-serine: step 1/1.

Subunit structure

Homodimer By similarity.

Subcellular location

Cytoplasm By similarity HAMAP MF_00051.

Sequence similarities

Belongs to the SHMT family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 426426Pyridoxal-phosphate-dependent serine hydroxymethyltransferase
PRO_1000195441

Regions

Region126 – 1283Substrate binding By similarity

Sites

Binding site361Pyridoxal phosphate By similarity
Binding site561Pyridoxal phosphate By similarity
Binding site581Substrate By similarity
Binding site651Substrate binding By similarity
Binding site661Pyridoxal phosphate By similarity
Binding site1221Substrate By similarity
Binding site1771Pyridoxal phosphate By similarity
Binding site2051Pyridoxal phosphate By similarity
Binding site2301Pyridoxal phosphate By similarity
Binding site2371Pyridoxal phosphate By similarity
Binding site2631Pyridoxal phosphate; via amide nitrogen and carbonyl oxygen By similarity
Binding site3631Pyridoxal phosphate By similarity

Amino acid modifications

Modified residue2311N6-(pyridoxal phosphate)lysine By similarity

Sequences

Sequence LengthMass (Da)Tools
B8HR59 [UniParc].

Last modified March 3, 2009. Version 1.
Checksum: E0A4846DC00FE859

FASTA42646,515
        10         20         30         40         50         60 
MTRTNLDFLT ETDPAIAGLL QQELQRQQDH LELIASENFT SAAVLAAQGS VLTNKYAEGL 

        70         80         90        100        110        120 
PGKRYYGGCE YIDKVEQLAI DRAKDLFQAA HANVQPHSGA QANFAVFLAL LQPGDTIMGM 

       130        140        150        160        170        180 
DLSHGGHLTH GSPVNVSGKW FRVVHYGVDP VTEQLDYEKI RQLAHQHRPK LIICGYSAYP 

       190        200        210        220        230        240 
RIIQFDQFRA IADEVGAYLL ADMAHIAGLV ATGHHPSPIP VCDVVTTTTH KTLRGPRGGL 

       250        260        270        280        290        300 
ILTRDPELGK KLDKAVFPGN QGGPLEHVIA AKAVAFGEAL RPEFKTYSAQ VIKNAQALSQ 

       310        320        330        340        350        360 
QLQQRGFKIV SNGTDNHLLL VDLRSIGMTG KLADQLVSEV NITANKNTVP FDPESPFVTS 

       370        380        390        400        410        420 
GLRLGSAAMT TRGMGTPEFI EIANIIADRL LNPEDLAIVQ ECRQRVAQLC DRFPLYPHLS 


RVPVLS

« Hide

References

[1]"Complete sequence of chromosome Cyanothece sp. PCC 7425."
US DOE Joint Genome Institute
Lucas S., Copeland A., Lapidus A., Glavina del Rio T., Dalin E., Tice H., Bruce D., Goodwin L., Pitluck S., Sims D., Meineke L., Brettin T., Detter J.C., Han C., Larimer F., Land M., Hauser L., Kyrpides N. expand/collapse author list , Ovchinnikova G., Liberton M., Stoeckel J., Banerjee A., Singh A., Page L., Sato H., Zhao L., Sherman L., Pakrasi H.B., Richardson P.
Submitted (JAN-2009) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: PCC 7425 / ATCC 29141.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		CP001344 Genomic DNA. Translation: ACL44047.1.
RefSeqYP_002482408.1. NC_011884.1.

3D structure databases

ProteinModelPortalB8HR59.
ModBaseSearch...

Protein-protein interaction databases

STRINGB8HR59.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID7287601.
GenomeReviewsGene locus Cyan7425_1678 in contig CP001344_GR.
KEGGcyn:Cyan7425_1678.
PATRIC21564309. VBICyaSp30657_1675.

Organism-specific databases

CMRSearch...

Phylogenomic databases

HOGENOMHBG301263.
OMARIDFERI.
PhylomeDBB8HR59.
ProtClustDBPRK00011.

Family and domain databases

HAMAPMF_00051. SHMT.
[Tree]
InterProIPR015424. PyrdxlP-dep_Trfase_major_dom.
IPR015421. PyrdxlP-dep_Trfase_major_sub1.
IPR015422. PyrdxlP-dep_Trfase_major_sub2.
IPR001085. Ser_HO-MeTrfase.
IPR019798. Ser_HO-MeTrfase_PLP_BS.
[Graphical view]
Gene3DG3DSA:3.40.640.10. PyrdxlP-dep_Trfase_major_sub1. 1 hit.
G3DSA:3.90.1150.10. PyrdxlP-dep_Trfase_major_sub2. 1 hit.
KOK00600.
PANTHERPTHR11680. Gly_HO-Metrfase. 1 hit.
PfamPF00464. SHMT. 1 hit.
[Graphical view]
PIRSFPIRSF000412. SHMT. 1 hit.
SUPFAMSSF53383. PyrdxlP-dep_Trfase_major. 1 hit.
PROSITEPS00096. SHMT. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameGLYA_CYAP4
AccessionPrimary (citable) accession number: B8HR59
Entry history
Integrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: March 3, 2009
Last modified: January 25, 2012
This is version 23 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

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