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Protein

Phosphoribosylformylglycinamidine cyclo-ligase

Gene

purM

Organism
Cyanothece sp. (strain PCC 7425 / ATCC 29141)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

ATP + 2-(formamido)-N(1)-(5-phospho-D-ribosyl)acetamidine = ADP + phosphate + 5-amino-1-(5-phospho-D-ribosyl)imidazole.UniRule annotation

Pathway:iIMP biosynthesis via de novo pathway

This protein is involved in step 2 of the subpathway that synthesizes 5-amino-1-(5-phospho-D-ribosyl)imidazole from N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide.UniRule annotation
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. Phosphoribosylformylglycinamidine synthase subunit PurL (purL), Phosphoribosylformylglycinamidine synthase subunit PurS (purS), Phosphoribosylformylglycinamidine synthase subunit PurQ (purQ)
  2. Phosphoribosylformylglycinamidine cyclo-ligase (purM)
This subpathway is part of the pathway IMP biosynthesis via de novo pathway, which is itself part of Purine metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes 5-amino-1-(5-phospho-D-ribosyl)imidazole from N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide, the pathway IMP biosynthesis via de novo pathway and in Purine metabolism.

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

Purine biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciCSP395961:GJDE-3171-MONOMER.
UniPathwayiUPA00074; UER00129.

Names & Taxonomyi

Protein namesi
Recommended name:
Phosphoribosylformylglycinamidine cyclo-ligaseUniRule annotation (EC:6.3.3.1UniRule annotation)
Alternative name(s):
AIR synthaseUniRule annotation
AIRSUniRule annotation
Phosphoribosyl-aminoimidazole synthetaseUniRule annotation
Gene namesi
Name:purMUniRule annotation
Ordered Locus Names:Cyan7425_3341
OrganismiCyanothece sp. (strain PCC 7425 / ATCC 29141)
Taxonomic identifieri395961 [NCBI]
Taxonomic lineageiBacteriaCyanobacteriaOscillatoriophycideaeChroococcalesCyanothece
ProteomesiUP000002511 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 367367Phosphoribosylformylglycinamidine cyclo-ligasePRO_1000148276Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi395961.Cyan7425_3341.

Structurei

3D structure databases

ProteinModelPortaliB8HPV7.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the AIR synthase family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0150.
HOGENOMiHOG000229090.
KOiK01933.
OMAiNHCVNDI.
OrthoDBiEOG61CM1V.

Family and domain databases

Gene3Di3.30.1330.10. 1 hit.
3.90.650.10. 1 hit.
HAMAPiMF_00741. AIRS.
InterProiIPR010918. AIR_synth_C_dom.
IPR000728. AIR_synth_N_dom.
IPR004733. PurM_cligase.
IPR016188. PurM_N-like.
[Graphical view]
PfamiPF00586. AIRS. 1 hit.
PF02769. AIRS_C. 1 hit.
[Graphical view]
SUPFAMiSSF55326. SSF55326. 1 hit.
SSF56042. SSF56042. 2 hits.
TIGRFAMsiTIGR00878. purM. 1 hit.

Sequencei

Sequence statusi: Complete.

B8HPV7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDYRDAGVDI QAGRDFVNRI KALVQQTTRS EVLGGLGGFG GCFQLPEGYR
60 70 80 90 100
QPVLVSGADG VGTKLKIAQA LNQHHTIGID LVAMCVNDVL TCGAEPLFFL
110 120 130 140 150
DYLATGHLEP EALTDVIAGI AQGCQQAGCA LLGGETAEMP GFYPVGEYDL
160 170 180 190 200
AGFCVGIVEK AEMLNGSQVR MGDRAIGLAS SGVHSNGYSL VRKIVDEFIG
210 220 230 240 250
PAAREQSEAG ATEALVSPWS ITPWAEREDL PPVDRQQSLG AALLTPTQIY
260 270 280 290 300
VKPVLAAQRS GLTLHGMAHI TGGGLPENLP RCLGEGQSVQ LDPQSWPAPP
310 320 330 340 350
IFDWIASTGS VPTAAMFDTF NMGIGFVLIV PPDEVESALR WFNTQQCPAY
360
GIGEVVAGAG EVLGLPG
Length:367
Mass (Da):38,605
Last modified:March 3, 2009 - v1
Checksum:i49D02BCD86FDD178
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001344 Genomic DNA. Translation: ACL45666.1.
RefSeqiWP_012628727.1. NC_011884.1.

Genome annotation databases

EnsemblBacteriaiACL45666; ACL45666; Cyan7425_3341.
KEGGicyn:Cyan7425_3341.
PATRICi21567639. VBICyaSp30657_3323.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001344 Genomic DNA. Translation: ACL45666.1.
RefSeqiWP_012628727.1. NC_011884.1.

3D structure databases

ProteinModelPortaliB8HPV7.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi395961.Cyan7425_3341.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiACL45666; ACL45666; Cyan7425_3341.
KEGGicyn:Cyan7425_3341.
PATRICi21567639. VBICyaSp30657_3323.

Phylogenomic databases

eggNOGiCOG0150.
HOGENOMiHOG000229090.
KOiK01933.
OMAiNHCVNDI.
OrthoDBiEOG61CM1V.

Enzyme and pathway databases

UniPathwayiUPA00074; UER00129.
BioCyciCSP395961:GJDE-3171-MONOMER.

Family and domain databases

Gene3Di3.30.1330.10. 1 hit.
3.90.650.10. 1 hit.
HAMAPiMF_00741. AIRS.
InterProiIPR010918. AIR_synth_C_dom.
IPR000728. AIR_synth_N_dom.
IPR004733. PurM_cligase.
IPR016188. PurM_N-like.
[Graphical view]
PfamiPF00586. AIRS. 1 hit.
PF02769. AIRS_C. 1 hit.
[Graphical view]
SUPFAMiSSF55326. SSF55326. 1 hit.
SSF56042. SSF56042. 2 hits.
TIGRFAMsiTIGR00878. purM. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. "Novel metabolic attributes of the genus Cyanothece, comprising a group of unicellular nitrogen-fixing Cyanobacteria."
    Bandyopadhyay A., Elvitigala T., Welsh E., Stockel J., Liberton M., Min H., Sherman L.A., Pakrasi H.B.
    MBio 2:E214-E214(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: PCC 7425 / ATCC 29141.

Entry informationi

Entry nameiPUR5_CYAP4
AccessioniPrimary (citable) accession number: B8HPV7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: March 3, 2009
Last modified: July 22, 2015
This is version 46 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.