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Protein

Biotin synthase

Gene

bioB

Organism
Cyanothece sp. (strain PCC 7425 / ATCC 29141)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the conversion of dethiobiotin (DTB) to biotin by the insertion of a sulfur atom into dethiobiotin via a radical-based mechanism.UniRule annotation

Catalytic activityi

Dethiobiotin + sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine + 2 reduced [2Fe-2S] ferredoxin = biotin + (sulfur carrier) + 2 L-methionine + 2 5'-deoxyadenosine + 2 oxidized [2Fe-2S] ferredoxin.UniRule annotation

Cofactori

Protein has several cofactor binding sites:
  • [4Fe-4S] clusterUniRule annotationNote: Binds 1 [4Fe-4S] cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.UniRule annotation
  • [2Fe-2S] clusterUniRule annotationNote: Binds 1 [2Fe-2S] cluster. The cluster is coordinated with 3 cysteines and 1 arginine.UniRule annotation

Pathwayi: biotin biosynthesis

This protein is involved in step 2 of the subpathway that synthesizes biotin from 7,8-diaminononanoate.UniRule annotation
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. ATP-dependent dethiobiotin synthetase BioD (bioD)
  2. Biotin synthase (bioB)
This subpathway is part of the pathway biotin biosynthesis, which is itself part of Cofactor biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes biotin from 7,8-diaminononanoate, the pathway biotin biosynthesis and in Cofactor biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi91Iron-sulfur 1 (4Fe-4S-S-AdoMet)UniRule annotation1
Metal bindingi95Iron-sulfur 1 (4Fe-4S-S-AdoMet)UniRule annotation1
Metal bindingi98Iron-sulfur 1 (4Fe-4S-S-AdoMet)UniRule annotation1
Metal bindingi136Iron-sulfur 2 (2Fe-2S)UniRule annotation1
Metal bindingi173Iron-sulfur 2 (2Fe-2S)UniRule annotation1
Metal bindingi233Iron-sulfur 2 (2Fe-2S)UniRule annotation1
Metal bindingi303Iron-sulfur 2 (2Fe-2S)UniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionTransferase
Biological processBiotin biosynthesis
Ligand2Fe-2S, 4Fe-4S, Iron, Iron-sulfur, Metal-binding, S-adenosyl-L-methionine

Enzyme and pathway databases

UniPathwayiUPA00078; UER00162

Names & Taxonomyi

Protein namesi
Recommended name:
Biotin synthaseUniRule annotation (EC:2.8.1.6UniRule annotation)
Gene namesi
Name:bioBUniRule annotation
Ordered Locus Names:Cyan7425_1531
OrganismiCyanothece sp. (strain PCC 7425 / ATCC 29141)
Taxonomic identifieri395961 [NCBI]
Taxonomic lineageiBacteriaCyanobacteriaOscillatoriophycideaeOscillatorialesCyanothecaceaeCyanothece
Proteomesi
  • UP000002511 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003813381 – 372Biotin synthaseAdd BLAST372

Proteomic databases

PRIDEiB8HPP0

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Protein-protein interaction databases

STRINGi395961.Cyan7425_1531

Structurei

3D structure databases

ProteinModelPortaliB8HPP0
SMRiB8HPP0
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the radical SAM superfamily. Biotin synthase family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4107QSQ Bacteria
COG0502 LUCA
HOGENOMiHOG000239958
KOiK01012
OrthoDBiPOG091H01DF

Family and domain databases

Gene3Di3.20.20.70, 1 hit
HAMAPiMF_01694 BioB, 1 hit
InterProiView protein in InterPro
IPR013785 Aldolase_TIM
IPR010722 BATS_dom
IPR034416 BATS_domain_containing
IPR002684 Biotin_synth/BioAB
IPR024177 Biotin_synthase
IPR006638 Elp3/MiaB/NifB
IPR007197 rSAM
PANTHERiPTHR22976 PTHR22976, 1 hit
PfamiView protein in Pfam
PF06968 BATS, 1 hit
PF04055 Radical_SAM, 1 hit
PIRSFiPIRSF001619 Biotin_synth, 1 hit
SFLDiSFLDG01060 BATS_domain_containing, 1 hit
SFLDG01278 biotin_synthase_like, 1 hit
SFLDS00029 Radical_SAM, 1 hit
SMARTiView protein in SMART
SM00876 BATS, 1 hit
SM00729 Elp3, 1 hit
TIGRFAMsiTIGR00433 bioB, 1 hit

Sequencei

Sequence statusi: Complete.

B8HPP0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVATPLPPTQ SPRHPSLQEL SLSELQTWLD ELAERIITGE TLDRATAIAL
60 70 80 90 100
TEIEGEEKIL ALCAAADRVR QACCGNTVDL CSIVNIKSGN CSENCSFCAQ
110 120 130 140 150
SAHHPGQDSP IYGMKTPEDI LAQARAAAAA GAKRFCLVSQ GRGPKYNSPK
160 170 180 190 200
STEFEQILAT VRAIIAETNI KPCCALGEVT PEQAQALKEA GVSRYNHNLE
210 220 230 240 250
SSENFFPEVA TTHSWRDRVE TIKTLKAAGI QACTGGILGL GESWQDRVDL
260 270 280 290 300
ALALRELEVE SVPLNLLNPR AGTLLGEQSK LDPFTALKAI AIFRLILPQQ
310 320 330 340 350
IIRYAGGREA VMGELQALGL KAGINAMLVG HYLTTMGQPP EQDHAMLAGL
360 370
GLQGGEAPIP GEYVRASQVR EL
Length:372
Mass (Da):39,943
Last modified:July 28, 2009 - v2
Checksum:iBD917B3B95C63E26
GO

Sequence cautioni

The sequence ACL43901 differs from that shown. Reason: Erroneous initiation.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001344 Genomic DNA Translation: ACL43901.1 Different initiation.

Genome annotation databases

EnsemblBacteriaiACL43901; ACL43901; Cyan7425_1531
KEGGicyn:Cyan7425_1531

Similar proteinsi

Entry informationi

Entry nameiBIOB_CYAP4
AccessioniPrimary (citable) accession number: B8HPP0
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: July 28, 2009
Last modified: May 23, 2018
This is version 56 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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