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B8HPP0

- BIOB_CYAP4

UniProt

B8HPP0 - BIOB_CYAP4

Protein

Biotin synthase

Gene

bioB

Organism
Cyanothece sp. (strain PCC 7425 / ATCC 29141)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 40 (01 Oct 2014)
      Sequence version 2 (28 Jul 2009)
      Previous versions | rss
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    Functioni

    Catalyzes the conversion of dethiobiotin (DTB) to biotin by the insertion of a sulfur atom into dethiobiotin via a radical-based mechanism.UniRule annotation

    Catalytic activityi

    Dethiobiotin + sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine = biotin + (sulfur carrier) + 2 L-methionine + 2 5'-deoxyadenosine.UniRule annotation

    Cofactori

    Binds 1 4Fe-4S cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.UniRule annotation
    Binds 1 2Fe-2S cluster. The cluster is coordinated with 3 cysteines and 1 arginine.UniRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi91 – 911Iron-sulfur 1 (4Fe-4S-S-AdoMet)UniRule annotation
    Metal bindingi95 – 951Iron-sulfur 1 (4Fe-4S-S-AdoMet)UniRule annotation
    Metal bindingi98 – 981Iron-sulfur 1 (4Fe-4S-S-AdoMet)UniRule annotation
    Metal bindingi136 – 1361Iron-sulfur 2 (2Fe-2S)UniRule annotation
    Metal bindingi173 – 1731Iron-sulfur 2 (2Fe-2S)UniRule annotation
    Metal bindingi233 – 2331Iron-sulfur 2 (2Fe-2S)UniRule annotation
    Metal bindingi303 – 3031Iron-sulfur 2 (2Fe-2S)UniRule annotation

    GO - Molecular functioni

    1. 2 iron, 2 sulfur cluster binding Source: UniProtKB-KW
    2. 4 iron, 4 sulfur cluster binding Source: UniProtKB-KW
    3. biotin synthase activity Source: UniProtKB-HAMAP
    4. iron ion binding Source: UniProtKB-HAMAP

    GO - Biological processi

    1. biotin biosynthetic process Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Transferase

    Keywords - Biological processi

    Biotin biosynthesis

    Keywords - Ligandi

    2Fe-2S, 4Fe-4S, Iron, Iron-sulfur, Metal-binding, S-adenosyl-L-methionine

    Enzyme and pathway databases

    BioCyciCSP395961:GJDE-1350-MONOMER.
    UniPathwayiUPA00078; UER00162.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Biotin synthaseUniRule annotation (EC:2.8.1.6UniRule annotation)
    Gene namesi
    Name:bioBUniRule annotation
    Ordered Locus Names:Cyan7425_1531
    OrganismiCyanothece sp. (strain PCC 7425 / ATCC 29141)
    Taxonomic identifieri395961 [NCBI]
    Taxonomic lineageiBacteriaCyanobacteriaOscillatoriophycideaeChroococcalesCyanothece
    ProteomesiUP000002511: Chromosome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 372372Biotin synthasePRO_0000381338Add
    BLAST

    Interactioni

    Subunit structurei

    Homodimer.UniRule annotation

    Protein-protein interaction databases

    STRINGi395961.Cyan7425_1531.

    Structurei

    3D structure databases

    ProteinModelPortaliB8HPP0.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the radical SAM superfamily. Biotin synthase family.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0502.
    HOGENOMiHOG000239958.
    KOiK01012.
    OrthoDBiEOG622PMP.

    Family and domain databases

    Gene3Di3.20.20.70. 1 hit.
    HAMAPiMF_01694. BioB.
    InterProiIPR013785. Aldolase_TIM.
    IPR010722. BATS_dom.
    IPR002684. Biotin_synth/BioAB.
    IPR024177. Biotin_synthase.
    IPR006638. Elp3/MiaB/NifB.
    IPR007197. rSAM.
    [Graphical view]
    PfamiPF06968. BATS. 1 hit.
    PF04055. Radical_SAM. 1 hit.
    [Graphical view]
    PIRSFiPIRSF001619. Biotin_synth. 1 hit.
    SMARTiSM00876. BATS. 1 hit.
    SM00729. Elp3. 1 hit.
    [Graphical view]
    TIGRFAMsiTIGR00433. bioB. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    B8HPP0-1 [UniParc]FASTAAdd to Basket

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    MVATPLPPTQ SPRHPSLQEL SLSELQTWLD ELAERIITGE TLDRATAIAL    50
    TEIEGEEKIL ALCAAADRVR QACCGNTVDL CSIVNIKSGN CSENCSFCAQ 100
    SAHHPGQDSP IYGMKTPEDI LAQARAAAAA GAKRFCLVSQ GRGPKYNSPK 150
    STEFEQILAT VRAIIAETNI KPCCALGEVT PEQAQALKEA GVSRYNHNLE 200
    SSENFFPEVA TTHSWRDRVE TIKTLKAAGI QACTGGILGL GESWQDRVDL 250
    ALALRELEVE SVPLNLLNPR AGTLLGEQSK LDPFTALKAI AIFRLILPQQ 300
    IIRYAGGREA VMGELQALGL KAGINAMLVG HYLTTMGQPP EQDHAMLAGL 350
    GLQGGEAPIP GEYVRASQVR EL 372
    Length:372
    Mass (Da):39,943
    Last modified:July 28, 2009 - v2
    Checksum:iBD917B3B95C63E26
    GO

    Sequence cautioni

    The sequence ACL43901.1 differs from that shown. Reason: Erroneous initiation.

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP001344 Genomic DNA. Translation: ACL43901.1. Different initiation.
    RefSeqiWP_012626992.1. NC_011884.1.
    YP_002482262.1. NC_011884.1.

    Genome annotation databases

    EnsemblBacteriaiACL43901; ACL43901; Cyan7425_1531.
    GeneIDi7287454.
    KEGGicyn:Cyan7425_1531.
    PATRICi21564015. VBICyaSp30657_1528.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP001344 Genomic DNA. Translation: ACL43901.1 . Different initiation.
    RefSeqi WP_012626992.1. NC_011884.1.
    YP_002482262.1. NC_011884.1.

    3D structure databases

    ProteinModelPortali B8HPP0.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 395961.Cyan7425_1531.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai ACL43901 ; ACL43901 ; Cyan7425_1531 .
    GeneIDi 7287454.
    KEGGi cyn:Cyan7425_1531.
    PATRICi 21564015. VBICyaSp30657_1528.

    Phylogenomic databases

    eggNOGi COG0502.
    HOGENOMi HOG000239958.
    KOi K01012.
    OrthoDBi EOG622PMP.

    Enzyme and pathway databases

    UniPathwayi UPA00078 ; UER00162 .
    BioCyci CSP395961:GJDE-1350-MONOMER.

    Family and domain databases

    Gene3Di 3.20.20.70. 1 hit.
    HAMAPi MF_01694. BioB.
    InterProi IPR013785. Aldolase_TIM.
    IPR010722. BATS_dom.
    IPR002684. Biotin_synth/BioAB.
    IPR024177. Biotin_synthase.
    IPR006638. Elp3/MiaB/NifB.
    IPR007197. rSAM.
    [Graphical view ]
    Pfami PF06968. BATS. 1 hit.
    PF04055. Radical_SAM. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF001619. Biotin_synth. 1 hit.
    SMARTi SM00876. BATS. 1 hit.
    SM00729. Elp3. 1 hit.
    [Graphical view ]
    TIGRFAMsi TIGR00433. bioB. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Novel metabolic attributes of the genus Cyanothece, comprising a group of unicellular nitrogen-fixing Cyanobacteria."
      Bandyopadhyay A., Elvitigala T., Welsh E., Stockel J., Liberton M., Min H., Sherman L.A., Pakrasi H.B.
      MBio 2:E214-E214(2011) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: PCC 7425 / ATCC 29141.

    Entry informationi

    Entry nameiBIOB_CYAP4
    AccessioniPrimary (citable) accession number: B8HPP0
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 28, 2009
    Last sequence update: July 28, 2009
    Last modified: October 1, 2014
    This is version 40 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3