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B8HPP0

- BIOB_CYAP4

UniProt

B8HPP0 - BIOB_CYAP4

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Protein
Biotin synthase
Gene
bioB, Cyan7425_1531
Organism
Cyanothece sp. (strain PCC 7425 / ATCC 29141)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes the conversion of dethiobiotin (DTB) to biotin by the insertion of a sulfur atom into dethiobiotin via a radical-based mechanism By similarity.UniRule annotation

Catalytic activityi

Dethiobiotin + sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine = biotin + (sulfur carrier) + 2 L-methionine + 2 5'-deoxyadenosine.UniRule annotation

Cofactori

Binds 1 4Fe-4S cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine By similarity.
Binds 1 2Fe-2S cluster. The cluster is coordinated with 3 cysteines and 1 arginine By similarity.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi91 – 911Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
Metal bindingi95 – 951Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
Metal bindingi98 – 981Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
Metal bindingi136 – 1361Iron-sulfur 2 (2Fe-2S) By similarity
Metal bindingi173 – 1731Iron-sulfur 2 (2Fe-2S) By similarity
Metal bindingi233 – 2331Iron-sulfur 2 (2Fe-2S) By similarity
Metal bindingi303 – 3031Iron-sulfur 2 (2Fe-2S) By similarity

GO - Molecular functioni

  1. 2 iron, 2 sulfur cluster binding Source: UniProtKB-KW
  2. 4 iron, 4 sulfur cluster binding Source: UniProtKB-KW
  3. biotin synthase activity Source: UniProtKB-HAMAP
  4. iron ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. biotin biosynthetic process Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Biotin biosynthesis

Keywords - Ligandi

2Fe-2S, 4Fe-4S, Iron, Iron-sulfur, Metal-binding, S-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciCSP395961:GJDE-1350-MONOMER.
UniPathwayiUPA00078; UER00162.

Names & Taxonomyi

Protein namesi
Recommended name:
Biotin synthase (EC:2.8.1.6)
Gene namesi
Name:bioB
Ordered Locus Names:Cyan7425_1531
OrganismiCyanothece sp. (strain PCC 7425 / ATCC 29141)
Taxonomic identifieri395961 [NCBI]
Taxonomic lineageiBacteriaCyanobacteriaOscillatoriophycideaeChroococcalesCyanothece
ProteomesiUP000002511: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 372372Biotin synthaseUniRule annotation
PRO_0000381338Add
BLAST

Interactioni

Subunit structurei

Homodimer By similarity.UniRule annotation

Protein-protein interaction databases

STRINGi395961.Cyan7425_1531.

Structurei

3D structure databases

ProteinModelPortaliB8HPP0.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0502.
HOGENOMiHOG000239958.
KOiK01012.
OrthoDBiEOG622PMP.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
HAMAPiMF_01694. BioB.
InterProiIPR013785. Aldolase_TIM.
IPR010722. BATS_dom.
IPR002684. Biotin_synth/BioAB.
IPR024177. Biotin_synthase.
IPR006638. Elp3/MiaB/NifB.
IPR007197. rSAM.
[Graphical view]
PfamiPF06968. BATS. 1 hit.
PF04055. Radical_SAM. 1 hit.
[Graphical view]
PIRSFiPIRSF001619. Biotin_synth. 1 hit.
SMARTiSM00876. BATS. 1 hit.
SM00729. Elp3. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00433. bioB. 1 hit.

Sequencei

Sequence statusi: Complete.

B8HPP0-1 [UniParc]FASTAAdd to Basket

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MVATPLPPTQ SPRHPSLQEL SLSELQTWLD ELAERIITGE TLDRATAIAL    50
TEIEGEEKIL ALCAAADRVR QACCGNTVDL CSIVNIKSGN CSENCSFCAQ 100
SAHHPGQDSP IYGMKTPEDI LAQARAAAAA GAKRFCLVSQ GRGPKYNSPK 150
STEFEQILAT VRAIIAETNI KPCCALGEVT PEQAQALKEA GVSRYNHNLE 200
SSENFFPEVA TTHSWRDRVE TIKTLKAAGI QACTGGILGL GESWQDRVDL 250
ALALRELEVE SVPLNLLNPR AGTLLGEQSK LDPFTALKAI AIFRLILPQQ 300
IIRYAGGREA VMGELQALGL KAGINAMLVG HYLTTMGQPP EQDHAMLAGL 350
GLQGGEAPIP GEYVRASQVR EL 372
Length:372
Mass (Da):39,943
Last modified:July 28, 2009 - v2
Checksum:iBD917B3B95C63E26
GO

Sequence cautioni

The sequence ACL43901.1 differs from that shown. Reason: Erroneous initiation.

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP001344 Genomic DNA. Translation: ACL43901.1. Different initiation.
RefSeqiWP_012626992.1. NC_011884.1.
YP_002482262.1. NC_011884.1.

Genome annotation databases

EnsemblBacteriaiACL43901; ACL43901; Cyan7425_1531.
GeneIDi7287454.
KEGGicyn:Cyan7425_1531.
PATRICi21564015. VBICyaSp30657_1528.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP001344 Genomic DNA. Translation: ACL43901.1 . Different initiation.
RefSeqi WP_012626992.1. NC_011884.1.
YP_002482262.1. NC_011884.1.

3D structure databases

ProteinModelPortali B8HPP0.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 395961.Cyan7425_1531.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ACL43901 ; ACL43901 ; Cyan7425_1531 .
GeneIDi 7287454.
KEGGi cyn:Cyan7425_1531.
PATRICi 21564015. VBICyaSp30657_1528.

Phylogenomic databases

eggNOGi COG0502.
HOGENOMi HOG000239958.
KOi K01012.
OrthoDBi EOG622PMP.

Enzyme and pathway databases

UniPathwayi UPA00078 ; UER00162 .
BioCyci CSP395961:GJDE-1350-MONOMER.

Family and domain databases

Gene3Di 3.20.20.70. 1 hit.
HAMAPi MF_01694. BioB.
InterProi IPR013785. Aldolase_TIM.
IPR010722. BATS_dom.
IPR002684. Biotin_synth/BioAB.
IPR024177. Biotin_synthase.
IPR006638. Elp3/MiaB/NifB.
IPR007197. rSAM.
[Graphical view ]
Pfami PF06968. BATS. 1 hit.
PF04055. Radical_SAM. 1 hit.
[Graphical view ]
PIRSFi PIRSF001619. Biotin_synth. 1 hit.
SMARTi SM00876. BATS. 1 hit.
SM00729. Elp3. 1 hit.
[Graphical view ]
TIGRFAMsi TIGR00433. bioB. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "Novel metabolic attributes of the genus Cyanothece, comprising a group of unicellular nitrogen-fixing Cyanobacteria."
    Bandyopadhyay A., Elvitigala T., Welsh E., Stockel J., Liberton M., Min H., Sherman L.A., Pakrasi H.B.
    MBio 2:E214-E214(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: PCC 7425 / ATCC 29141.

Entry informationi

Entry nameiBIOB_CYAP4
AccessioniPrimary (citable) accession number: B8HPP0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: July 28, 2009
Last modified: September 3, 2014
This is version 39 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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