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Protein

Biotin synthase

Gene

bioB

Organism
Cyanothece sp. (strain PCC 7425 / ATCC 29141)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the conversion of dethiobiotin (DTB) to biotin by the insertion of a sulfur atom into dethiobiotin via a radical-based mechanism.UniRule annotation

Catalytic activityi

Dethiobiotin + sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine = biotin + (sulfur carrier) + 2 L-methionine + 2 5'-deoxyadenosine.UniRule annotation

Cofactori

Protein has several cofactor binding sites:
  • [4Fe-4S] clusterUniRule annotationNote: Binds 1 [4Fe-4S] cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.UniRule annotation
  • [2Fe-2S] clusterUniRule annotationNote: Binds 1 [2Fe-2S] cluster. The cluster is coordinated with 3 cysteines and 1 arginine.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi91 – 911Iron-sulfur 1 (4Fe-4S-S-AdoMet)UniRule annotation
Metal bindingi95 – 951Iron-sulfur 1 (4Fe-4S-S-AdoMet)UniRule annotation
Metal bindingi98 – 981Iron-sulfur 1 (4Fe-4S-S-AdoMet)UniRule annotation
Metal bindingi136 – 1361Iron-sulfur 2 (2Fe-2S)UniRule annotation
Metal bindingi173 – 1731Iron-sulfur 2 (2Fe-2S)UniRule annotation
Metal bindingi233 – 2331Iron-sulfur 2 (2Fe-2S)UniRule annotation
Metal bindingi303 – 3031Iron-sulfur 2 (2Fe-2S)UniRule annotation

GO - Molecular functioni

  1. 2 iron, 2 sulfur cluster binding Source: UniProtKB-KW
  2. 4 iron, 4 sulfur cluster binding Source: UniProtKB-KW
  3. biotin synthase activity Source: UniProtKB-HAMAP
  4. iron ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. biotin biosynthetic process Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Biotin biosynthesis

Keywords - Ligandi

2Fe-2S, 4Fe-4S, Iron, Iron-sulfur, Metal-binding, S-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciCSP395961:GJDE-1350-MONOMER.
UniPathwayiUPA00078; UER00162.

Names & Taxonomyi

Protein namesi
Recommended name:
Biotin synthaseUniRule annotation (EC:2.8.1.6UniRule annotation)
Gene namesi
Name:bioBUniRule annotation
Ordered Locus Names:Cyan7425_1531
OrganismiCyanothece sp. (strain PCC 7425 / ATCC 29141)
Taxonomic identifieri395961 [NCBI]
Taxonomic lineageiBacteriaCyanobacteriaOscillatoriophycideaeChroococcalesCyanothece
ProteomesiUP000002511: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 372372Biotin synthasePRO_0000381338Add
BLAST

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Protein-protein interaction databases

STRINGi395961.Cyan7425_1531.

Structurei

3D structure databases

ProteinModelPortaliB8HPP0.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the radical SAM superfamily. Biotin synthase family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0502.
HOGENOMiHOG000239958.
KOiK01012.
OrthoDBiEOG622PMP.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
HAMAPiMF_01694. BioB.
InterProiIPR013785. Aldolase_TIM.
IPR010722. BATS_dom.
IPR002684. Biotin_synth/BioAB.
IPR024177. Biotin_synthase.
IPR006638. Elp3/MiaB/NifB.
IPR007197. rSAM.
[Graphical view]
PfamiPF06968. BATS. 1 hit.
PF04055. Radical_SAM. 1 hit.
[Graphical view]
PIRSFiPIRSF001619. Biotin_synth. 1 hit.
SMARTiSM00876. BATS. 1 hit.
SM00729. Elp3. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00433. bioB. 1 hit.

Sequencei

Sequence statusi: Complete.

B8HPP0-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MVATPLPPTQ SPRHPSLQEL SLSELQTWLD ELAERIITGE TLDRATAIAL
60 70 80 90 100
TEIEGEEKIL ALCAAADRVR QACCGNTVDL CSIVNIKSGN CSENCSFCAQ
110 120 130 140 150
SAHHPGQDSP IYGMKTPEDI LAQARAAAAA GAKRFCLVSQ GRGPKYNSPK
160 170 180 190 200
STEFEQILAT VRAIIAETNI KPCCALGEVT PEQAQALKEA GVSRYNHNLE
210 220 230 240 250
SSENFFPEVA TTHSWRDRVE TIKTLKAAGI QACTGGILGL GESWQDRVDL
260 270 280 290 300
ALALRELEVE SVPLNLLNPR AGTLLGEQSK LDPFTALKAI AIFRLILPQQ
310 320 330 340 350
IIRYAGGREA VMGELQALGL KAGINAMLVG HYLTTMGQPP EQDHAMLAGL
360 370
GLQGGEAPIP GEYVRASQVR EL
Length:372
Mass (Da):39,943
Last modified:July 28, 2009 - v2
Checksum:iBD917B3B95C63E26
GO

Sequence cautioni

The sequence ACL43901.1 differs from that shown. Reason: Erroneous initiation. Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001344 Genomic DNA. Translation: ACL43901.1. Different initiation.
RefSeqiWP_012626992.1. NC_011884.1.
YP_002482262.1. NC_011884.1.

Genome annotation databases

EnsemblBacteriaiACL43901; ACL43901; Cyan7425_1531.
GeneIDi7287454.
KEGGicyn:Cyan7425_1531.
PATRICi21564015. VBICyaSp30657_1528.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001344 Genomic DNA. Translation: ACL43901.1. Different initiation.
RefSeqiWP_012626992.1. NC_011884.1.
YP_002482262.1. NC_011884.1.

3D structure databases

ProteinModelPortaliB8HPP0.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi395961.Cyan7425_1531.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiACL43901; ACL43901; Cyan7425_1531.
GeneIDi7287454.
KEGGicyn:Cyan7425_1531.
PATRICi21564015. VBICyaSp30657_1528.

Phylogenomic databases

eggNOGiCOG0502.
HOGENOMiHOG000239958.
KOiK01012.
OrthoDBiEOG622PMP.

Enzyme and pathway databases

UniPathwayiUPA00078; UER00162.
BioCyciCSP395961:GJDE-1350-MONOMER.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
HAMAPiMF_01694. BioB.
InterProiIPR013785. Aldolase_TIM.
IPR010722. BATS_dom.
IPR002684. Biotin_synth/BioAB.
IPR024177. Biotin_synthase.
IPR006638. Elp3/MiaB/NifB.
IPR007197. rSAM.
[Graphical view]
PfamiPF06968. BATS. 1 hit.
PF04055. Radical_SAM. 1 hit.
[Graphical view]
PIRSFiPIRSF001619. Biotin_synth. 1 hit.
SMARTiSM00876. BATS. 1 hit.
SM00729. Elp3. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00433. bioB. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. "Novel metabolic attributes of the genus Cyanothece, comprising a group of unicellular nitrogen-fixing Cyanobacteria."
    Bandyopadhyay A., Elvitigala T., Welsh E., Stockel J., Liberton M., Min H., Sherman L.A., Pakrasi H.B.
    MBio 2:E214-E214(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: PCC 7425 / ATCC 29141.

Entry informationi

Entry nameiBIOB_CYAP4
AccessioniPrimary (citable) accession number: B8HPP0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: July 28, 2009
Last modified: January 7, 2015
This is version 42 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.