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B8HPG4 (PUR9_CYAP4) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 39. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Bifunctional purine biosynthesis protein PurH

Including the following 2 domains:

  1. Phosphoribosylaminoimidazolecarboxamide formyltransferase
    EC=2.1.2.3
    Alternative name(s):
    AICAR transformylase
  2. IMP cyclohydrolase
    EC=3.5.4.10
    Alternative name(s):
    ATIC
    IMP synthase
    Inosinicase
Gene names
Name:purH
Ordered Locus Names:Cyan7425_1454
OrganismCyanothece sp. (strain PCC 7425 / ATCC 29141) [Complete proteome] [HAMAP]
Taxonomic identifier395961 [NCBI]
Taxonomic lineageBacteriaCyanobacteriaOscillatoriophycideaeChroococcalesCyanothece

Protein attributes

Sequence length514 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

10-formyltetrahydrofolate + 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide = tetrahydrofolate + 5-formamido-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide. HAMAP-Rule MF_00139

IMP + H2O = 5-formamido-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide. HAMAP-Rule MF_00139

Pathway

Purine metabolism; IMP biosynthesis via de novo pathway; 5-formamido-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide from 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide (10-formyl THF route): step 1/1. HAMAP-Rule MF_00139

Purine metabolism; IMP biosynthesis via de novo pathway; IMP from 5-formamido-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide: step 1/1.

Domain

The IMP cyclohydrolase activity resides in the N-terminal region By similarity. HAMAP-Rule MF_00139

Sequence similarities

Belongs to the PurH family.

Ontologies

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 514514Bifunctional purine biosynthesis protein PurH HAMAP-Rule MF_00139
PRO_1000122956

Sequences

Sequence LengthMass (Da)Tools
B8HPG4 [UniParc].

Last modified March 3, 2009. Version 1.
Checksum: 8F2387A81B76FD3C

FASTA51454,790
        10         20         30         40         50         60 
MPPLALLSTS DKTGVVEFAR SLVNEFGFQL ISSGGTAKAL IAAGLPVTKV ADYTGSPEIL 

        70         80         90        100        110        120 
GGRVKTLHPR IHGGILARRD LPTDLQDLAA TGIEPIDLVV VNLYPFEQTI AQPDMTLSDA 

       130        140        150        160        170        180 
IEQIDIGGPA LLRASAKNFA HVTVLCSPEQ YGPCLAELRQ QQGQTSLIFR QSCAQKAFWH 

       190        200        210        220        230        240 
TATYDQAIAT YLTSSDPTEI SLPPRLSVTA TQFQSLRYGE NPHQAAAWYR TGTATGGWSG 

       250        260        270        280        290        300 
ATQLQGKELS YNNLVDLEAA RAIVAEFTEQ PAAVITKHTN PCGVAEGDSL VEAYRRAFQA 

       310        320        330        340        350        360 
DSTSAFGGIV ALNHPLDGET AKAMTETFLE CIVAPGCEPE AEAILKTKSN LRVLVLPDLS 

       370        380        390        400        410        420 
QGASLEIKAI AGGFLVQQPD RQPIQPQSWE VVSQTKPTEE QWQELIFAWK VAKHVKSNAI 

       430        440        450        460        470        480 
VVTADRVTLG VGAGQMNRVG SVQIALTQAG EKAQGAVLAS DGFFPFDDSV RAAAAAGIGA 

       490        500        510 
IVQPGGSKRD ADSIQAADQL GLVMVFTHTR HFLH 

« Hide

References

[1]"Novel metabolic attributes of the genus Cyanothece, comprising a group of unicellular nitrogen-fixing Cyanobacteria."
Bandyopadhyay A., Elvitigala T., Welsh E., Stockel J., Liberton M., Min H., Sherman L.A., Pakrasi H.B.
MBio 2:E214-E214(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: PCC 7425 / ATCC 29141.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP001344 Genomic DNA. Translation: ACL43825.1.
RefSeqYP_002482186.1. NC_011884.1.

3D structure databases

ProteinModelPortalB8HPG4.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING395961.Cyan7425_1454.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaACL43825; ACL43825; Cyan7425_1454.
GeneID7287376.
KEGGcyn:Cyan7425_1454.
PATRIC21563851. VBICyaSp30657_1447.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0138.
HOGENOMHOG000230373.
KOK00602.
OMADLLFAWK.
OrthoDBEOG6QCDFF.
ProtClustDBPRK00881.

Enzyme and pathway databases

BioCycCSP395961:GJDE-1272-MONOMER.
UniPathwayUPA00074; UER00133.
UPA00074; UER00135.

Family and domain databases

Gene3D3.40.140.20. 2 hits.
3.40.50.1380. 1 hit.
HAMAPMF_00139. PurH.
InterProIPR024051. AICAR_Tfase_dom.
IPR002695. AICARFT_IMPCHas.
IPR016193. Cytidine_deaminase-like.
IPR011607. MGS-like_dom.
[Graphical view]
PANTHERPTHR11692. PTHR11692. 1 hit.
PfamPF01808. AICARFT_IMPCHas. 1 hit.
PF02142. MGS. 1 hit.
[Graphical view]
PIRSFPIRSF000414. AICARFT_IMPCHas. 1 hit.
SMARTSM00798. AICARFT_IMPCHas. 1 hit.
SM00851. MGS. 1 hit.
[Graphical view]
SUPFAMSSF52335. SSF52335. 1 hit.
SSF53927. SSF53927. 1 hit.
TIGRFAMsTIGR00355. purH. 1 hit.
ProtoNetSearch...

Entry information

Entry namePUR9_CYAP4
AccessionPrimary (citable) accession number: B8HPG4
Entry history
Integrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: March 3, 2009
Last modified: February 19, 2014
This is version 39 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways