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Protein

Bifunctional purine biosynthesis protein PurH

Gene

purH

Organism
Cyanothece sp. (strain PCC 7425 / ATCC 29141)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

10-formyltetrahydrofolate + 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide = tetrahydrofolate + 5-formamido-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide.UniRule annotation
IMP + H2O = 5-formamido-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide.UniRule annotation

Pathwayi

GO - Molecular functioni

  1. IMP cyclohydrolase activity Source: UniProtKB-HAMAP
  2. phosphoribosylaminoimidazolecarboxamide formyltransferase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. 'de novo' IMP biosynthetic process Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Transferase

Keywords - Biological processi

Purine biosynthesis

Enzyme and pathway databases

BioCyciCSP395961:GJDE-1272-MONOMER.
UniPathwayiUPA00074; UER00133.
UPA00074; UER00135.

Names & Taxonomyi

Protein namesi
Recommended name:
Bifunctional purine biosynthesis protein PurHUniRule annotation
Including the following 2 domains:
Phosphoribosylaminoimidazolecarboxamide formyltransferaseUniRule annotation (EC:2.1.2.3UniRule annotation)
Alternative name(s):
AICAR transformylaseUniRule annotation
IMP cyclohydrolaseUniRule annotation (EC:3.5.4.10UniRule annotation)
Alternative name(s):
ATICUniRule annotation
IMP synthaseUniRule annotation
InosinicaseUniRule annotation
Gene namesi
Name:purHUniRule annotation
Ordered Locus Names:Cyan7425_1454
OrganismiCyanothece sp. (strain PCC 7425 / ATCC 29141)
Taxonomic identifieri395961 [NCBI]
Taxonomic lineageiBacteriaCyanobacteriaOscillatoriophycideaeChroococcalesCyanothece
ProteomesiUP000002511: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 514514Bifunctional purine biosynthesis protein PurHPRO_1000122956Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi395961.Cyan7425_1454.

Structurei

3D structure databases

ProteinModelPortaliB8HPG4.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domaini

The IMP cyclohydrolase activity resides in the N-terminal region.UniRule annotation

Sequence similaritiesi

Belongs to the PurH family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0138.
HOGENOMiHOG000230373.
KOiK00602.
OMAiPCGVAEG.
OrthoDBiEOG6QCDFF.

Family and domain databases

Gene3Di3.40.140.20. 2 hits.
3.40.50.1380. 1 hit.
HAMAPiMF_00139. PurH.
InterProiIPR024051. AICAR_Tfase_dom.
IPR002695. AICARFT_IMPCHas.
IPR016193. Cytidine_deaminase-like.
IPR011607. MGS-like_dom.
[Graphical view]
PANTHERiPTHR11692. PTHR11692. 1 hit.
PfamiPF01808. AICARFT_IMPCHas. 1 hit.
PF02142. MGS. 1 hit.
[Graphical view]
PIRSFiPIRSF000414. AICARFT_IMPCHas. 1 hit.
SMARTiSM00798. AICARFT_IMPCHas. 1 hit.
SM00851. MGS. 1 hit.
[Graphical view]
SUPFAMiSSF52335. SSF52335. 1 hit.
SSF53927. SSF53927. 1 hit.
TIGRFAMsiTIGR00355. purH. 1 hit.

Sequencei

Sequence statusi: Complete.

B8HPG4-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MPPLALLSTS DKTGVVEFAR SLVNEFGFQL ISSGGTAKAL IAAGLPVTKV
60 70 80 90 100
ADYTGSPEIL GGRVKTLHPR IHGGILARRD LPTDLQDLAA TGIEPIDLVV
110 120 130 140 150
VNLYPFEQTI AQPDMTLSDA IEQIDIGGPA LLRASAKNFA HVTVLCSPEQ
160 170 180 190 200
YGPCLAELRQ QQGQTSLIFR QSCAQKAFWH TATYDQAIAT YLTSSDPTEI
210 220 230 240 250
SLPPRLSVTA TQFQSLRYGE NPHQAAAWYR TGTATGGWSG ATQLQGKELS
260 270 280 290 300
YNNLVDLEAA RAIVAEFTEQ PAAVITKHTN PCGVAEGDSL VEAYRRAFQA
310 320 330 340 350
DSTSAFGGIV ALNHPLDGET AKAMTETFLE CIVAPGCEPE AEAILKTKSN
360 370 380 390 400
LRVLVLPDLS QGASLEIKAI AGGFLVQQPD RQPIQPQSWE VVSQTKPTEE
410 420 430 440 450
QWQELIFAWK VAKHVKSNAI VVTADRVTLG VGAGQMNRVG SVQIALTQAG
460 470 480 490 500
EKAQGAVLAS DGFFPFDDSV RAAAAAGIGA IVQPGGSKRD ADSIQAADQL
510
GLVMVFTHTR HFLH
Length:514
Mass (Da):54,790
Last modified:March 3, 2009 - v1
Checksum:i8F2387A81B76FD3C
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001344 Genomic DNA. Translation: ACL43825.1.
RefSeqiWP_012626916.1. NC_011884.1.
YP_002482186.1. NC_011884.1.

Genome annotation databases

EnsemblBacteriaiACL43825; ACL43825; Cyan7425_1454.
GeneIDi7287376.
KEGGicyn:Cyan7425_1454.
PATRICi21563851. VBICyaSp30657_1447.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001344 Genomic DNA. Translation: ACL43825.1.
RefSeqiWP_012626916.1. NC_011884.1.
YP_002482186.1. NC_011884.1.

3D structure databases

ProteinModelPortaliB8HPG4.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi395961.Cyan7425_1454.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiACL43825; ACL43825; Cyan7425_1454.
GeneIDi7287376.
KEGGicyn:Cyan7425_1454.
PATRICi21563851. VBICyaSp30657_1447.

Phylogenomic databases

eggNOGiCOG0138.
HOGENOMiHOG000230373.
KOiK00602.
OMAiPCGVAEG.
OrthoDBiEOG6QCDFF.

Enzyme and pathway databases

UniPathwayiUPA00074; UER00133.
UPA00074; UER00135.
BioCyciCSP395961:GJDE-1272-MONOMER.

Family and domain databases

Gene3Di3.40.140.20. 2 hits.
3.40.50.1380. 1 hit.
HAMAPiMF_00139. PurH.
InterProiIPR024051. AICAR_Tfase_dom.
IPR002695. AICARFT_IMPCHas.
IPR016193. Cytidine_deaminase-like.
IPR011607. MGS-like_dom.
[Graphical view]
PANTHERiPTHR11692. PTHR11692. 1 hit.
PfamiPF01808. AICARFT_IMPCHas. 1 hit.
PF02142. MGS. 1 hit.
[Graphical view]
PIRSFiPIRSF000414. AICARFT_IMPCHas. 1 hit.
SMARTiSM00798. AICARFT_IMPCHas. 1 hit.
SM00851. MGS. 1 hit.
[Graphical view]
SUPFAMiSSF52335. SSF52335. 1 hit.
SSF53927. SSF53927. 1 hit.
TIGRFAMsiTIGR00355. purH. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. "Novel metabolic attributes of the genus Cyanothece, comprising a group of unicellular nitrogen-fixing Cyanobacteria."
    Bandyopadhyay A., Elvitigala T., Welsh E., Stockel J., Liberton M., Min H., Sherman L.A., Pakrasi H.B.
    MBio 2:E214-E214(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: PCC 7425 / ATCC 29141.

Entry informationi

Entry nameiPUR9_CYAP4
AccessioniPrimary (citable) accession number: B8HPG4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: March 3, 2009
Last modified: January 7, 2015
This is version 43 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Multifunctional enzyme, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.