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B8HNL6 (BIOD_CYAP4) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 25. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
ATP-dependent dethiobiotin synthetase BioD
EC=6.3.3.3
Alternative name(s):
DTB synthetase
Short name=DTBS
Dethiobiotin synthase
Gene names
Name:bioD
Ordered Locus Names:Cyan7425_1373
OrganismCyanothece sp. (strain PCC 7425 / ATCC 29141) [Complete proteome] [HAMAP]
Taxonomic identifier395961 [NCBI]
Taxonomic lineageBacteria › Cyanobacteria › Chroococcales › Cyanothece

Protein attributes

Sequence length226 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes a mechanistically unusual reaction, the ATP-dependent insertion of CO2 between the N7 and N8 nitrogen atoms of 7,8-diaminopelargonic acid (DAPA) to form an ureido ring By similarity. HAMAP MF_00336

Catalytic activity

ATP + 7,8-diaminononanoate + CO2 = ADP + phosphate + dethiobiotin. HAMAP MF_00336

Cofactor

Magnesium By similarity. HAMAP MF_00336

Pathway

Cofactor biosynthesis; biotin biosynthesis; biotin from 7,8-diaminononanoate: step 1/2. HAMAP MF_00336

Subcellular location

Cytoplasm By similarity HAMAP MF_00336.

Sequence similarities

Belongs to the dethiobiotin synthetase family.

Ontologies

Keywords
   Biological processBiotin biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Magnesium
Metal-binding
Nucleotide-binding
   Molecular functionLigase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological processbiotin biosynthetic process

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular functionATP binding

Inferred from electronic annotation. Source: UniProtKB-KW

dethiobiotin synthase activity

Inferred from electronic annotation. Source: EC

magnesium ion binding

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 226226ATP-dependent dethiobiotin synthetase BioD HAMAP MF_00336
PRO_1000133213

Regions

Nucleotide binding12 – 176ATP By similarity
Nucleotide binding108 – 1114ATP By similarity
Nucleotide binding168 – 1692ATP By similarity
Nucleotide binding200 – 2023ATP By similarity

Sites

Metal binding121Magnesium 1 By similarity
Metal binding161Magnesium 2 By similarity
Metal binding471Magnesium 2 By similarity
Metal binding1081Magnesium 2 By similarity
Binding site431Substrate By similarity
Binding site471ATP By similarity
Binding site2071ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
B8HNL6 [UniParc].

Last modified March 3, 2009. Version 1.
Checksum: 64C9EA922398E36A

FASTA22624,327
        10         20         30         40         50         60 
MFTLLITGTD TDAGKTVLTT ALAAYWRTYF PSQSVALLKP LQSGVGDREL YRELFALNQP 

        70         80         90        100        110        120 
PEEINPLYFQ APLAPPLAAE LEQRSIDLSL PWQTLVKLRQ SYDRVLVEGV GGLGSPITWE 

       130        140        150        160        170        180 
LTVADLARDW GLATLLVVPV KLGAIGQAVA NVALARQAGV NLKGIVLNCV QPRSETEIAQ 

       190        200        210        220 
WAPADLITNL TQIPVLGVLP YLGDRTNLAQ LAAAAAQLDL EAFLSA

« Hide

References

[1]"Complete sequence of chromosome Cyanothece sp. PCC 7425."
US DOE Joint Genome Institute
Lucas S., Copeland A., Lapidus A., Glavina del Rio T., Dalin E., Tice H., Bruce D., Goodwin L., Pitluck S., Sims D., Meineke L., Brettin T., Detter J.C., Han C., Larimer F., Land M., Hauser L., Kyrpides N. expand/collapse author list , Ovchinnikova G., Liberton M., Stoeckel J., Banerjee A., Singh A., Page L., Sato H., Zhao L., Sherman L., Pakrasi H.B., Richardson P.
Submitted (JAN-2009) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: PCC 7425 / ATCC 29141.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		CP001344 Genomic DNA. Translation: ACL43747.1.
RefSeqYP_002482108.1. NC_011884.1.

3D structure databases

ProteinModelPortalB8HNL6.
ModBaseSearch...

Protein-protein interaction databases

STRINGB8HNL6.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID7287293.
GenomeReviewsGene locus Cyan7425_1373 in contig CP001344_GR.
KEGGcyn:Cyan7425_1373.
PATRIC21563683. VBICyaSp30657_1365.

Organism-specific databases

CMRSearch...

Phylogenomic databases

HOGENOMHBG650065.
OMAALAAYWQ.
PhylomeDBB8HNL6.
ProtClustDBPRK00090.

Family and domain databases

HAMAPMF_00336. BioD.
[Tree]
InterProIPR002586. CbiA_P_synth.
IPR004472. DTB_synth_BioD.
[Graphical view]
KOK01935.
PfamPF01656. CbiA. 1 hit.
[Graphical view]
PIRSFPIRSF006755. DTB_synth. 1 hit.
TIGRFAMsTIGR00347. BioD. 1 hit.
ProtoNetSearch...

Entry information

Entry nameBIOD_CYAP4
AccessionPrimary (citable) accession number: B8HNL6
Entry history
Integrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: March 3, 2009
Last modified: January 25, 2012
This is version 25 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

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