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B8HMX3

- SYI_CYAP4

UniProt

B8HMX3 - SYI_CYAP4

Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Cyanothece sp. (strain PCC 7425 / ATCC 29141)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 41 (01 Oct 2014)
      Sequence version 1 (03 Mar 2009)
      Previous versions | rss
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    Functioni

    Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

    Catalytic activityi

    ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

    Cofactori

    Binds 1 zinc ion per subunit.UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei563 – 5631Aminoacyl-adenylateUniRule annotation
    Binding sitei607 – 6071ATPUniRule annotation
    Metal bindingi926 – 9261ZincUniRule annotation
    Metal bindingi929 – 9291ZincUniRule annotation
    Metal bindingi946 – 9461ZincUniRule annotation
    Metal bindingi949 – 9491ZincUniRule annotation

    GO - Molecular functioni

    1. aminoacyl-tRNA editing activity Source: InterPro
    2. ATP binding Source: UniProtKB-HAMAP
    3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
    4. zinc ion binding Source: UniProtKB-HAMAP

    GO - Biological processi

    1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Aminoacyl-tRNA synthetase, Ligase

    Keywords - Biological processi

    Protein biosynthesis

    Keywords - Ligandi

    ATP-binding, Metal-binding, Nucleotide-binding, Zinc

    Enzyme and pathway databases

    BioCyciCSP395961:GJDE-2942-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
    Alternative name(s):
    Isoleucyl-tRNA synthetaseUniRule annotation
    Short name:
    IleRSUniRule annotation
    Gene namesi
    Name:ileSUniRule annotation
    Ordered Locus Names:Cyan7425_3114
    OrganismiCyanothece sp. (strain PCC 7425 / ATCC 29141)
    Taxonomic identifieri395961 [NCBI]
    Taxonomic lineageiBacteriaCyanobacteriaOscillatoriophycideaeChroococcalesCyanothece
    ProteomesiUP000002511: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 955955Isoleucine--tRNA ligasePRO_1000189146Add
    BLAST

    Interactioni

    Subunit structurei

    Monomer.UniRule annotation

    Protein-protein interaction databases

    STRINGi395961.Cyan7425_3114.

    Structurei

    3D structure databases

    ProteinModelPortaliB8HMX3.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi60 – 7011"HIGH" regionAdd
    BLAST
    Motifi604 – 6085"KMSKS" region

    Domaini

    IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

    Sequence similaritiesi

    Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 1 subfamily.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0060.
    HOGENOMiHOG000246402.
    KOiK01870.
    OMAiKPVHWCL.
    OrthoDBiEOG644ZM1.

    Family and domain databases

    Gene3Di1.10.730.10. 1 hit.
    3.40.50.620. 2 hits.
    3.90.740.10. 1 hit.
    HAMAPiMF_02002. Ile_tRNA_synth_type1.
    InterProiIPR001412. aa-tRNA-synth_I_CS.
    IPR002300. aa-tRNA-synth_Ia.
    IPR002301. Ile-tRNA-ligase.
    IPR023585. Ile-tRNA-ligase_type1.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR009080. tRNAsynth_1a_anticodon-bd.
    IPR013155. V/L/I-tRNA-synth_anticodon-bd.
    IPR009008. Val/Leu/Ile-tRNA-synth_edit.
    IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
    [Graphical view]
    PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
    PfamiPF08264. Anticodon_1. 1 hit.
    PF00133. tRNA-synt_1. 1 hit.
    PF06827. zf-FPG_IleRS. 1 hit.
    [Graphical view]
    PRINTSiPR00984. TRNASYNTHILE.
    SUPFAMiSSF47323. SSF47323. 1 hit.
    SSF50677. SSF50677. 1 hit.
    TIGRFAMsiTIGR00392. ileS. 1 hit.
    PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    B8HMX3-1 [UniParc]FASTAAdd to Basket

    « Hide

    MTEPGSYKET VNLPQTAFDM RANAVKREPE LQKFWAENQI YENLSQENPG    50
    DPFILHDGPP YANGDLHIGH ALNKILKDII NKYWMLQGRK VRYVPGWDCH 100
    GLPIELKVLQ GMKSEQRAAL TPLSLRQAAK EFALQTVERQ KQSFKRYGVW 150
    GEWDNPYLTL KPEYEAAQIG VFGQMVLKGY IYRGLKPVYW SPSSQTALAE 200
    AELEYPEGHT SRSIYVSFAL TSLSATAEAV LKDYLPNLKV AIWTTTPWTI 250
    PGNLAVALNP DLTYAVVEKD GSYLLVANEL VERLETTLGV SLPIKTTIAG 300
    QALEHSTYQH PLFDRQGPLV MGDYVTTDSG TGLVHTAPGH GQEDYVVGQH 350
    YGLPILSPVD GNGRFTEEAG QFAGLKVLDE GNEAVIQALQ QVGALLKEEA 400
    YSHKYPYDWR TKKPVILRAT EQWFASVEGF REAALQAIAQ VQWIPAQGEN 450
    RITAMVSERS DWCISRQRNW GVPIPVFYDE ETGEPLLNAE TIAHVQAIVA 500
    EKGSDAWWEL SIEELLPQAY RNNGRRYRKG TDTMDVWFDS GSSWAAVLEQ 550
    REQLRYPAEM YLEGSDQHRG WFQSSLLTSV ATHGIAPYKT VLTHGFVLDE 600
    QGRKMSKSLG NVVDPAIVIE GGKNQKEDPP YGADILRLWV SSVDYSSDVP 650
    LGKNILKQMA DIYRKIRNTA RFLLGNLHDF DPANNKVEYD RLPQLDRYML 700
    HRIVEVFTEV NEAFTSYQFF RFFQTVQNFC VVDLSNFYLD IAKDRLYISA 750
    PDDFRRRSCQ TVLWIALENL AKAIAPVVPH LAEDIWQFLP YPTPYKSVFE 800
    AGWVSLDATW TNPDPQLLET FEQVRLLRFK VNECLEKLRV NKVIGASLEA 850
    KVLLYIADPE KRQQWQNLNP PALAAISGVD ELRYFFLTSQ VELLNSPDQL 900
    SELKSEYRFE FGDSSGGVLP ADGQKCDRCW NYSTYVGKDD KHLLLCERCV 950
    AVMER 955
    Length:955
    Mass (Da):108,361
    Last modified:March 3, 2009 - v1
    Checksum:iD41AC42C142D7C1A
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP001344 Genomic DNA. Translation: ACL45442.1.
    RefSeqiWP_012628505.1. NC_011884.1.
    YP_002483803.1. NC_011884.1.

    Genome annotation databases

    EnsemblBacteriaiACL45442; ACL45442; Cyan7425_3114.
    GeneIDi7289048.
    KEGGicyn:Cyan7425_3114.
    PATRICi21567193. VBICyaSp30657_3102.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP001344 Genomic DNA. Translation: ACL45442.1 .
    RefSeqi WP_012628505.1. NC_011884.1.
    YP_002483803.1. NC_011884.1.

    3D structure databases

    ProteinModelPortali B8HMX3.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 395961.Cyan7425_3114.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai ACL45442 ; ACL45442 ; Cyan7425_3114 .
    GeneIDi 7289048.
    KEGGi cyn:Cyan7425_3114.
    PATRICi 21567193. VBICyaSp30657_3102.

    Phylogenomic databases

    eggNOGi COG0060.
    HOGENOMi HOG000246402.
    KOi K01870.
    OMAi KPVHWCL.
    OrthoDBi EOG644ZM1.

    Enzyme and pathway databases

    BioCyci CSP395961:GJDE-2942-MONOMER.

    Family and domain databases

    Gene3Di 1.10.730.10. 1 hit.
    3.40.50.620. 2 hits.
    3.90.740.10. 1 hit.
    HAMAPi MF_02002. Ile_tRNA_synth_type1.
    InterProi IPR001412. aa-tRNA-synth_I_CS.
    IPR002300. aa-tRNA-synth_Ia.
    IPR002301. Ile-tRNA-ligase.
    IPR023585. Ile-tRNA-ligase_type1.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR009080. tRNAsynth_1a_anticodon-bd.
    IPR013155. V/L/I-tRNA-synth_anticodon-bd.
    IPR009008. Val/Leu/Ile-tRNA-synth_edit.
    IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
    [Graphical view ]
    PANTHERi PTHR11946:SF9. PTHR11946:SF9. 1 hit.
    Pfami PF08264. Anticodon_1. 1 hit.
    PF00133. tRNA-synt_1. 1 hit.
    PF06827. zf-FPG_IleRS. 1 hit.
    [Graphical view ]
    PRINTSi PR00984. TRNASYNTHILE.
    SUPFAMi SSF47323. SSF47323. 1 hit.
    SSF50677. SSF50677. 1 hit.
    TIGRFAMsi TIGR00392. ileS. 1 hit.
    PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Novel metabolic attributes of the genus Cyanothece, comprising a group of unicellular nitrogen-fixing Cyanobacteria."
      Bandyopadhyay A., Elvitigala T., Welsh E., Stockel J., Liberton M., Min H., Sherman L.A., Pakrasi H.B.
      MBio 2:E214-E214(2011) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: PCC 7425 / ATCC 29141.

    Entry informationi

    Entry nameiSYI_CYAP4
    AccessioniPrimary (citable) accession number: B8HMX3
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 28, 2009
    Last sequence update: March 3, 2009
    Last modified: October 1, 2014
    This is version 41 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Aminoacyl-tRNA synthetases
      List of aminoacyl-tRNA synthetase entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3