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Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Cyanothece sp. (strain PCC 7425 / ATCC 29141)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Cofactori

Zn2+UniRule annotationNote: Binds 1 zinc ion per subunit.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei563Aminoacyl-adenylateUniRule annotation1
Binding sitei607ATPUniRule annotation1
Metal bindingi926ZincUniRule annotation1
Metal bindingi929ZincUniRule annotation1
Metal bindingi946ZincUniRule annotation1
Metal bindingi949ZincUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
Alternative name(s):
Isoleucyl-tRNA synthetaseUniRule annotation
Short name:
IleRSUniRule annotation
Gene namesi
Name:ileSUniRule annotation
Ordered Locus Names:Cyan7425_3114
OrganismiCyanothece sp. (strain PCC 7425 / ATCC 29141)
Taxonomic identifieri395961 [NCBI]
Taxonomic lineageiBacteriaCyanobacteriaOscillatoriophycideaeOscillatorialesCyanothecaceaeCyanothece
Proteomesi
  • UP000002511 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_10001891461 – 955Isoleucine--tRNA ligaseAdd BLAST955

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi395961.Cyan7425_3114.

Structurei

3D structure databases

ProteinModelPortaliB8HMX3.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi60 – 70"HIGH" regionAdd BLAST11
Motifi604 – 608"KMSKS" region5

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 1 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105C07. Bacteria.
COG0060. LUCA.
HOGENOMiHOG000246402.
KOiK01870.
OMAiTHGFIVD.
OrthoDBiPOG091H028I.

Family and domain databases

CDDicd07960. Anticodon_Ia_Ile_BEm. 1 hit.
Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02002. Ile_tRNA_synth_type1. 1 hit.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR033708. Anticodon_Ile_BEm.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_Ia_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_FPG/IleRS.
[Graphical view]
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

B8HMX3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTEPGSYKET VNLPQTAFDM RANAVKREPE LQKFWAENQI YENLSQENPG
60 70 80 90 100
DPFILHDGPP YANGDLHIGH ALNKILKDII NKYWMLQGRK VRYVPGWDCH
110 120 130 140 150
GLPIELKVLQ GMKSEQRAAL TPLSLRQAAK EFALQTVERQ KQSFKRYGVW
160 170 180 190 200
GEWDNPYLTL KPEYEAAQIG VFGQMVLKGY IYRGLKPVYW SPSSQTALAE
210 220 230 240 250
AELEYPEGHT SRSIYVSFAL TSLSATAEAV LKDYLPNLKV AIWTTTPWTI
260 270 280 290 300
PGNLAVALNP DLTYAVVEKD GSYLLVANEL VERLETTLGV SLPIKTTIAG
310 320 330 340 350
QALEHSTYQH PLFDRQGPLV MGDYVTTDSG TGLVHTAPGH GQEDYVVGQH
360 370 380 390 400
YGLPILSPVD GNGRFTEEAG QFAGLKVLDE GNEAVIQALQ QVGALLKEEA
410 420 430 440 450
YSHKYPYDWR TKKPVILRAT EQWFASVEGF REAALQAIAQ VQWIPAQGEN
460 470 480 490 500
RITAMVSERS DWCISRQRNW GVPIPVFYDE ETGEPLLNAE TIAHVQAIVA
510 520 530 540 550
EKGSDAWWEL SIEELLPQAY RNNGRRYRKG TDTMDVWFDS GSSWAAVLEQ
560 570 580 590 600
REQLRYPAEM YLEGSDQHRG WFQSSLLTSV ATHGIAPYKT VLTHGFVLDE
610 620 630 640 650
QGRKMSKSLG NVVDPAIVIE GGKNQKEDPP YGADILRLWV SSVDYSSDVP
660 670 680 690 700
LGKNILKQMA DIYRKIRNTA RFLLGNLHDF DPANNKVEYD RLPQLDRYML
710 720 730 740 750
HRIVEVFTEV NEAFTSYQFF RFFQTVQNFC VVDLSNFYLD IAKDRLYISA
760 770 780 790 800
PDDFRRRSCQ TVLWIALENL AKAIAPVVPH LAEDIWQFLP YPTPYKSVFE
810 820 830 840 850
AGWVSLDATW TNPDPQLLET FEQVRLLRFK VNECLEKLRV NKVIGASLEA
860 870 880 890 900
KVLLYIADPE KRQQWQNLNP PALAAISGVD ELRYFFLTSQ VELLNSPDQL
910 920 930 940 950
SELKSEYRFE FGDSSGGVLP ADGQKCDRCW NYSTYVGKDD KHLLLCERCV

AVMER
Length:955
Mass (Da):108,361
Last modified:March 3, 2009 - v1
Checksum:iD41AC42C142D7C1A
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001344 Genomic DNA. Translation: ACL45442.1.
RefSeqiWP_012628505.1. NC_011884.1.

Genome annotation databases

EnsemblBacteriaiACL45442; ACL45442; Cyan7425_3114.
KEGGicyn:Cyan7425_3114.
PATRICi21567193. VBICyaSp30657_3102.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001344 Genomic DNA. Translation: ACL45442.1.
RefSeqiWP_012628505.1. NC_011884.1.

3D structure databases

ProteinModelPortaliB8HMX3.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi395961.Cyan7425_3114.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiACL45442; ACL45442; Cyan7425_3114.
KEGGicyn:Cyan7425_3114.
PATRICi21567193. VBICyaSp30657_3102.

Phylogenomic databases

eggNOGiENOG4105C07. Bacteria.
COG0060. LUCA.
HOGENOMiHOG000246402.
KOiK01870.
OMAiTHGFIVD.
OrthoDBiPOG091H028I.

Family and domain databases

CDDicd07960. Anticodon_Ia_Ile_BEm. 1 hit.
Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02002. Ile_tRNA_synth_type1. 1 hit.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR033708. Anticodon_Ile_BEm.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_Ia_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_FPG/IleRS.
[Graphical view]
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiSYI_CYAP4
AccessioniPrimary (citable) accession number: B8HMX3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: March 3, 2009
Last modified: November 2, 2016
This is version 52 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.