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B8HMX3

- SYI_CYAP4

UniProt

B8HMX3 - SYI_CYAP4

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Protein
Isoleucine--tRNA ligase
Gene
ileS, Cyan7425_3114
Organism
Cyanothece sp. (strain PCC 7425 / ATCC 29141)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile) By similarity.UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Cofactori

Binds 1 zinc ion per subunit By similarity.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei563 – 5631Aminoacyl-adenylate By similarity
Binding sitei607 – 6071ATP By similarity
Metal bindingi926 – 9261Zinc By similarity
Metal bindingi929 – 9291Zinc By similarity
Metal bindingi946 – 9461Zinc By similarity
Metal bindingi949 – 9491Zinc By similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-HAMAP
  2. aminoacyl-tRNA editing activity Source: InterPro
  3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
  4. zinc ion binding Source: UniProtKB-HAMAP
Complete GO annotation...

GO - Biological processi

  1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciCSP395961:GJDE-2942-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligase (EC:6.1.1.5)
Alternative name(s):
Isoleucyl-tRNA synthetase
Short name:
IleRS
Gene namesi
Name:ileS
Ordered Locus Names:Cyan7425_3114
OrganismiCyanothece sp. (strain PCC 7425 / ATCC 29141)
Taxonomic identifieri395961 [NCBI]
Taxonomic lineageiBacteriaCyanobacteriaOscillatoriophycideaeChroococcalesCyanothece
ProteomesiUP000002511: Chromosome

Subcellular locationi

Cytoplasm By similarity UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 955955Isoleucine--tRNA ligaseUniRule annotation
PRO_1000189146Add
BLAST

Interactioni

Subunit structurei

Monomer By similarity.UniRule annotation

Protein-protein interaction databases

STRINGi395961.Cyan7425_3114.

Structurei

3D structure databases

ProteinModelPortaliB8HMX3.

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi60 – 7011"HIGH" regionUniRule annotation
Add
BLAST
Motifi604 – 6085"KMSKS" regionUniRule annotation

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)) By similarity.UniRule annotation

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0060.
HOGENOMiHOG000246402.
KOiK01870.
OMAiKPVHWCL.
OrthoDBiEOG644ZM1.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02002. Ile_tRNA_synth_type1.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view]
PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

B8HMX3-1 [UniParc]FASTAAdd to Basket

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MTEPGSYKET VNLPQTAFDM RANAVKREPE LQKFWAENQI YENLSQENPG    50
DPFILHDGPP YANGDLHIGH ALNKILKDII NKYWMLQGRK VRYVPGWDCH 100
GLPIELKVLQ GMKSEQRAAL TPLSLRQAAK EFALQTVERQ KQSFKRYGVW 150
GEWDNPYLTL KPEYEAAQIG VFGQMVLKGY IYRGLKPVYW SPSSQTALAE 200
AELEYPEGHT SRSIYVSFAL TSLSATAEAV LKDYLPNLKV AIWTTTPWTI 250
PGNLAVALNP DLTYAVVEKD GSYLLVANEL VERLETTLGV SLPIKTTIAG 300
QALEHSTYQH PLFDRQGPLV MGDYVTTDSG TGLVHTAPGH GQEDYVVGQH 350
YGLPILSPVD GNGRFTEEAG QFAGLKVLDE GNEAVIQALQ QVGALLKEEA 400
YSHKYPYDWR TKKPVILRAT EQWFASVEGF REAALQAIAQ VQWIPAQGEN 450
RITAMVSERS DWCISRQRNW GVPIPVFYDE ETGEPLLNAE TIAHVQAIVA 500
EKGSDAWWEL SIEELLPQAY RNNGRRYRKG TDTMDVWFDS GSSWAAVLEQ 550
REQLRYPAEM YLEGSDQHRG WFQSSLLTSV ATHGIAPYKT VLTHGFVLDE 600
QGRKMSKSLG NVVDPAIVIE GGKNQKEDPP YGADILRLWV SSVDYSSDVP 650
LGKNILKQMA DIYRKIRNTA RFLLGNLHDF DPANNKVEYD RLPQLDRYML 700
HRIVEVFTEV NEAFTSYQFF RFFQTVQNFC VVDLSNFYLD IAKDRLYISA 750
PDDFRRRSCQ TVLWIALENL AKAIAPVVPH LAEDIWQFLP YPTPYKSVFE 800
AGWVSLDATW TNPDPQLLET FEQVRLLRFK VNECLEKLRV NKVIGASLEA 850
KVLLYIADPE KRQQWQNLNP PALAAISGVD ELRYFFLTSQ VELLNSPDQL 900
SELKSEYRFE FGDSSGGVLP ADGQKCDRCW NYSTYVGKDD KHLLLCERCV 950
AVMER 955
Length:955
Mass (Da):108,361
Last modified:March 3, 2009 - v1
Checksum:iD41AC42C142D7C1A
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP001344 Genomic DNA. Translation: ACL45442.1.
RefSeqiWP_012628505.1. NC_011884.1.
YP_002483803.1. NC_011884.1.

Genome annotation databases

EnsemblBacteriaiACL45442; ACL45442; Cyan7425_3114.
GeneIDi7289048.
KEGGicyn:Cyan7425_3114.
PATRICi21567193. VBICyaSp30657_3102.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP001344 Genomic DNA. Translation: ACL45442.1 .
RefSeqi WP_012628505.1. NC_011884.1.
YP_002483803.1. NC_011884.1.

3D structure databases

ProteinModelPortali B8HMX3.
ModBasei Search...

Protein-protein interaction databases

STRINGi 395961.Cyan7425_3114.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ACL45442 ; ACL45442 ; Cyan7425_3114 .
GeneIDi 7289048.
KEGGi cyn:Cyan7425_3114.
PATRICi 21567193. VBICyaSp30657_3102.

Phylogenomic databases

eggNOGi COG0060.
HOGENOMi HOG000246402.
KOi K01870.
OMAi KPVHWCL.
OrthoDBi EOG644ZM1.

Enzyme and pathway databases

BioCyci CSP395961:GJDE-2942-MONOMER.

Family and domain databases

Gene3Di 1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPi MF_02002. Ile_tRNA_synth_type1.
InterProi IPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view ]
PANTHERi PTHR11946:SF9. PTHR11946:SF9. 1 hit.
Pfami PF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view ]
PRINTSi PR00984. TRNASYNTHILE.
SUPFAMi SSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsi TIGR00392. ileS. 1 hit.
PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Novel metabolic attributes of the genus Cyanothece, comprising a group of unicellular nitrogen-fixing Cyanobacteria."
    Bandyopadhyay A., Elvitigala T., Welsh E., Stockel J., Liberton M., Min H., Sherman L.A., Pakrasi H.B.
    MBio 2:E214-E214(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: PCC 7425 / ATCC 29141.

Entry informationi

Entry nameiSYI_CYAP4
AccessioniPrimary (citable) accession number: B8HMX3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: March 3, 2009
Last modified: September 3, 2014
This is version 40 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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