B8HMJ9 (GLO2_CYAP4) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Hydroxyacylglutathione hydrolase
EC=3.1.2.6

Alternative name(s):
Glyoxalase II
Short name=Glx II

Gene names

Name:	gloB
Ordered Locus Names:	Cyan7425_1235

Organism

Cyanothece sp. (strain PCC 7425 / ATCC 29141) [Complete proteome] [HAMAP]

Taxonomic identifier

395961 [NCBI]

Taxonomic lineage

Bacteria › Cyanobacteria › Chroococcales › Cyanothece

Protein attributes

Sequence length	256 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Thiolesterase that catalyzes the hydrolysis of S-D-lactoyl-glutathione to form glutathione and D-lactic acid By similarity. HAMAP MF_01374
Catalytic activity	S-(2-hydroxyacyl)glutathione + H2O = glutathione + a 2-hydroxy carboxylate. HAMAP MF_01374
Cofactor	Binds 2 zinc ions per subunit By similarity. HAMAP MF_01374
Pathway	Secondary metabolite metabolism; methylglyoxal degradation; (R)-lactate from methylglyoxal: step 2/2. HAMAP MF_01374
Subunit structure	Monomer By similarity. HAMAP MF_01374
Sequence similarities	Belongs to the metallo-beta-lactamase superfamily. Glyoxalase II family.

Ontologies

Keywords
Ligand	Metal-binding Zinc
Molecular function	Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Molecular function	hydroxyacylglutathione hydrolase activity Inferred from electronic annotation. Source: EC zinc ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 256	256	Hydroxyacylglutathione hydrolase HAMAP MF_01374		PRO_1000184176
Sites
	Metal binding	54	1	Zinc 1 By similarity
	Metal binding	56	1	Zinc 1 By similarity
	Metal binding	58	1	Zinc 2 By similarity
	Metal binding	59	1	Zinc 2 By similarity
	Metal binding	113	1	Zinc 1 By similarity
	Metal binding	136	1	Zinc 1 By similarity
	Metal binding	136	1	Zinc 2 By similarity
	Metal binding	174	1	Zinc 2 By similarity

Sequences

	Sequence		Length	Mass (Da)
	B8HMJ9 [UniParc]. Last modified March 3, 2009. Version 1. Checksum: 7BFDA5C8EFEEEEAC	FASTA	256	29,054
	10 20 30 40 50 60 MQIDRLPVLS DNYIFLVLDP ERRQAAVVDP AVAAPVLERL RSLQFQLVAI FNTHHHHDHV 70 80 90 100 110 120 GGNQELLQHF PTAVVYGGAE DRGRIPGQQV FLQPGDRVNF AQRTAEVLFV PGHTRAHIAY 130 140 150 160 170 180 YFPPVNHSSG ELFCGDTLFA GGCGRLFEGT PAQMVNSLNQ LRQLPDNTRV WCAHEYTLKN 190 200 210 220 230 240 LQFALTIEPE NVELQTRLAQ VYLARQHFQP TVPSELGLEK RTNPFLRWDV PQVQQSVKGQ 250 DGIQTFTRLR GRKDQF

References

[1]

"Complete sequence of chromosome Cyanothece sp. PCC 7425." US DOE Joint Genome Institute Lucas S., Copeland A., Lapidus A., Glavina del Rio T., Dalin E., Tice H., Bruce D., Goodwin L., Pitluck S., Sims D., Meineke L., Brettin T., Detter J.C., Han C., Larimer F., Land M., Hauser L., Kyrpides N. , Ovchinnikova G., Liberton M., Stoeckel J., Banerjee A., Singh A., Page L., Sato H., Zhao L., Sherman L., Pakrasi H.B., Richardson P. Submitted (JAN-2009) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: PCC 7425 / ATCC 29141.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP001344 Genomic DNA. Translation: ACL43614.1.
RefSeq	YP_002481975.1. NC_011884.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	B8HMJ9.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	7287156.
GenomeReviews	Gene locus Cyan7425_1235 in contig CP001344_GR.
KEGG	cyn:Cyan7425_1235.
PATRIC	21563399. VBICyaSp30657_1223.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG753931.
OMA	IGCGRVF.
PhylomeDB	B8HMJ9.
Family and domain databases
HAMAP	MF_01374. Glyoxalase_2. [Tree]
InterPro	IPR001279. Beta-lactamas-like. IPR017782. Hydroxyacylglutathione_Hdrlase. [Graphical view]
KO	K01069.
PANTHER	PTHR11935:SF7. PTHR11935:SF7. 1 hit.
Pfam	PF00753. Lactamase_B. 1 hit. [Graphical view]
SMART	SM00849. Lactamase_B. 1 hit. [Graphical view]
TIGRFAMs	TIGR03413. GSH_gloB. 1 hit.
ProtoNet	Search...

Entry information

Entry name

GLO2_CYAP4

Accession

Primary (citable) accession number: B8HMJ9

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 28, 2009
Last sequence update:	March 3, 2009
Last modified:	January 25, 2012
This is version 22 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program