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B8HM05 (SYL_CYAP4) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 41. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Leucine--tRNA ligase

EC=6.1.1.4
Alternative name(s):
Leucyl-tRNA synthetase
Short name=LeuRS
Gene names
Name:leuS
Ordered Locus Names:Cyan7425_3031
OrganismCyanothece sp. (strain PCC 7425 / ATCC 29141) [Complete proteome] [HAMAP]
Taxonomic identifier395961 [NCBI]
Taxonomic lineageBacteriaCyanobacteriaOscillatoriophycideaeChroococcalesCyanothece

Protein attributes

Sequence length858 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu). HAMAP-Rule MF_00049

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00049.

Sequence similarities

Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
   Biological processProtein biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionAminoacyl-tRNA synthetase
Ligase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processleucyl-tRNA aminoacylation

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

aminoacyl-tRNA editing activity

Inferred from electronic annotation. Source: InterPro

leucine-tRNA ligase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 858858Leucine--tRNA ligase HAMAP-Rule MF_00049
PRO_1000199190

Regions

Motif42 – 5211"HIGH" region HAMAP-Rule MF_00049
Motif619 – 6235"KMSKS" region HAMAP-Rule MF_00049

Sites

Binding site6221ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
B8HM05 [UniParc].

Last modified March 3, 2009. Version 1.
Checksum: 0DAA53022B113E82

FASTA85897,261
        10         20         30         40         50         60 
MESRYQPTAI EEKWQQTWAE VGIDQTPTDR SKPKFYALSM FPYPSGNLHM GHVRNYTITD 

        70         80         90        100        110        120 
VIARVRRMQG YRVLHPMGWD AFGLPAENAA IKRGIHPAKW TADNITQMRS ELKRLGLSYD 

       130        140        150        160        170        180 
WNCELATCSP DYYRWTQWIF LQFYQAGLAY QKEAAVNWDP IDQTVLANEQ VDSEGRSWRS 

       190        200        210        220        230        240 
GAKVERKLLR QWFLKITDYA EELLQDLDQL TGWPEKVRKL QANWIGKSTG AYLEFPIVGR 

       250        260        270        280        290        300 
DEKISVYTTR PDTVYGVSYV VLAPEHPLTL QVTTKKQLKA VKSFIQEVTA ASEIERTAED 

       310        320        330        340        350        360 
QPKRGIATGG KAINPFTGAE IPIWIADYVL YEYGTGAVMG VPAHDSRDFK FAQTYQLPIR 

       370        380        390        400        410        420 
QVIIPPDPDL NEQPILQEAY TEPGLLINSG EFDGMPSTDA KAAIVAKAEA TGWGQARVQY 

       430        440        450        460        470        480 
RLRDWLISRQ RYWGAPIPVI HCPQCGIVPV PEADLPVVLP DDVQFSGRGP SPLAQLESWV 

       490        500        510        520        530        540 
KVNCPTCNTP ARRETDTMDT FIDSSWYYLR YPDAQNDQQV FDPAKTNDWL PVDQYVGGIE 

       550        560        570        580        590        600 
HAILHLLYSR FFTKVLRDRG LLNFDEPFQR LLTQGMVQGL TYTNPNRSDS SRYIPSNLVD 

       610        620        630        640        650        660 
PNDPKDPETG EPLEVSYQTM SKSKYNGVAP EEVINKYGAD TARMFILFKA PPEKDLEWDD 

       670        680        690        700        710        720 
ADVEGQFRFL NRVWRLVTQY PVIEPTTKQG DALAKEEKEL RRAIHTAIKE ITADLGEEYQ 

       730        740        750        760        770        780 
LNTAVSELMK LSNALTDASC KDSVIYTEGI ETLLLLLAPF APHISEELWQ QLGHTTSVHQ 

       790        800        810        820        830        840 
QSWPQVDPSA LIVDEITIVI QILGKTRGTI QVPASSSREE LEEYARQTPV AQRYLEGKTI 

       850 
KKVIVVPGKL VNFVVADA 

« Hide

References

[1]"Novel metabolic attributes of the genus Cyanothece, comprising a group of unicellular nitrogen-fixing Cyanobacteria."
Bandyopadhyay A., Elvitigala T., Welsh E., Stockel J., Liberton M., Min H., Sherman L.A., Pakrasi H.B.
MBio 2:E214-E214(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: PCC 7425 / ATCC 29141.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP001344 Genomic DNA. Translation: ACL45365.1.
RefSeqYP_002483726.1. NC_011884.1.

3D structure databases

ProteinModelPortalB8HM05.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING395961.Cyan7425_3031.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaACL45365; ACL45365; Cyan7425_3031.
GeneID7288964.
KEGGcyn:Cyan7425_3031.
PATRIC21567025. VBICyaSp30657_3020.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0495.
HOGENOMHOG000200747.
KOK01869.
OMAHISHELW.
OrthoDBEOG63Z74X.

Enzyme and pathway databases

BioCycCSP395961:GJDE-2858-MONOMER.

Family and domain databases

Gene3D1.10.730.10. 1 hit.
3.40.50.620. 3 hits.
3.90.740.10. 1 hit.
HAMAPMF_00049_B. Leu_tRNA_synth_B.
InterProIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase.
IPR025709. Leu_tRNA-synth_edit.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERPTHR11946:SF7. PTHR11946:SF7. 1 hit.
PfamPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 3 hits.
PF13603. tRNA-synt_1_2. 1 hit.
[Graphical view]
PRINTSPR00985. TRNASYNTHLEU.
SUPFAMSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsTIGR00396. leuS_bact. 1 hit.
PROSITEPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameSYL_CYAP4
AccessionPrimary (citable) accession number: B8HM05
Entry history
Integrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: March 3, 2009
Last modified: May 14, 2014
This is version 41 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries