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Protein

Leucine--tRNA ligase

Gene

leuS

Organism
Cyanothece sp. (strain PCC 7425 / ATCC 29141)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu).UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei622 – 6221ATPUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciCSP395961:GJDE-2858-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Leucine--tRNA ligaseUniRule annotation (EC:6.1.1.4UniRule annotation)
Alternative name(s):
Leucyl-tRNA synthetaseUniRule annotation
Short name:
LeuRSUniRule annotation
Gene namesi
Name:leuSUniRule annotation
Ordered Locus Names:Cyan7425_3031
OrganismiCyanothece sp. (strain PCC 7425 / ATCC 29141)
Taxonomic identifieri395961 [NCBI]
Taxonomic lineageiBacteriaCyanobacteriaOscillatoriophycideaeChroococcalesCyanothece
ProteomesiUP000002511 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 858858Leucine--tRNA ligasePRO_1000199190Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi395961.Cyan7425_3031.

Structurei

3D structure databases

ProteinModelPortaliB8HM05.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi42 – 5211"HIGH" regionAdd
BLAST
Motifi619 – 6235"KMSKS" region

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0495.
HOGENOMiHOG000200747.
KOiK01869.
OMAiQSSWYFL.
OrthoDBiEOG63Z74X.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 3 hits.
3.90.740.10. 1 hit.
HAMAPiMF_00049_B. Leu_tRNA_synth_B.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase.
IPR025709. Leu_tRNA-synth_edit.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERiPTHR11946:SF7. PTHR11946:SF7. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 3 hits.
PF13603. tRNA-synt_1_2. 1 hit.
[Graphical view]
PRINTSiPR00985. TRNASYNTHLEU.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00396. leuS_bact. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

B8HM05-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MESRYQPTAI EEKWQQTWAE VGIDQTPTDR SKPKFYALSM FPYPSGNLHM
60 70 80 90 100
GHVRNYTITD VIARVRRMQG YRVLHPMGWD AFGLPAENAA IKRGIHPAKW
110 120 130 140 150
TADNITQMRS ELKRLGLSYD WNCELATCSP DYYRWTQWIF LQFYQAGLAY
160 170 180 190 200
QKEAAVNWDP IDQTVLANEQ VDSEGRSWRS GAKVERKLLR QWFLKITDYA
210 220 230 240 250
EELLQDLDQL TGWPEKVRKL QANWIGKSTG AYLEFPIVGR DEKISVYTTR
260 270 280 290 300
PDTVYGVSYV VLAPEHPLTL QVTTKKQLKA VKSFIQEVTA ASEIERTAED
310 320 330 340 350
QPKRGIATGG KAINPFTGAE IPIWIADYVL YEYGTGAVMG VPAHDSRDFK
360 370 380 390 400
FAQTYQLPIR QVIIPPDPDL NEQPILQEAY TEPGLLINSG EFDGMPSTDA
410 420 430 440 450
KAAIVAKAEA TGWGQARVQY RLRDWLISRQ RYWGAPIPVI HCPQCGIVPV
460 470 480 490 500
PEADLPVVLP DDVQFSGRGP SPLAQLESWV KVNCPTCNTP ARRETDTMDT
510 520 530 540 550
FIDSSWYYLR YPDAQNDQQV FDPAKTNDWL PVDQYVGGIE HAILHLLYSR
560 570 580 590 600
FFTKVLRDRG LLNFDEPFQR LLTQGMVQGL TYTNPNRSDS SRYIPSNLVD
610 620 630 640 650
PNDPKDPETG EPLEVSYQTM SKSKYNGVAP EEVINKYGAD TARMFILFKA
660 670 680 690 700
PPEKDLEWDD ADVEGQFRFL NRVWRLVTQY PVIEPTTKQG DALAKEEKEL
710 720 730 740 750
RRAIHTAIKE ITADLGEEYQ LNTAVSELMK LSNALTDASC KDSVIYTEGI
760 770 780 790 800
ETLLLLLAPF APHISEELWQ QLGHTTSVHQ QSWPQVDPSA LIVDEITIVI
810 820 830 840 850
QILGKTRGTI QVPASSSREE LEEYARQTPV AQRYLEGKTI KKVIVVPGKL

VNFVVADA
Length:858
Mass (Da):97,261
Last modified:March 3, 2009 - v1
Checksum:i0DAA53022B113E82
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001344 Genomic DNA. Translation: ACL45365.1.
RefSeqiWP_012628431.1. NC_011884.1.
YP_002483726.1. NC_011884.1.

Genome annotation databases

EnsemblBacteriaiACL45365; ACL45365; Cyan7425_3031.
KEGGicyn:Cyan7425_3031.
PATRICi21567025. VBICyaSp30657_3020.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001344 Genomic DNA. Translation: ACL45365.1.
RefSeqiWP_012628431.1. NC_011884.1.
YP_002483726.1. NC_011884.1.

3D structure databases

ProteinModelPortaliB8HM05.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi395961.Cyan7425_3031.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiACL45365; ACL45365; Cyan7425_3031.
KEGGicyn:Cyan7425_3031.
PATRICi21567025. VBICyaSp30657_3020.

Phylogenomic databases

eggNOGiCOG0495.
HOGENOMiHOG000200747.
KOiK01869.
OMAiQSSWYFL.
OrthoDBiEOG63Z74X.

Enzyme and pathway databases

BioCyciCSP395961:GJDE-2858-MONOMER.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 3 hits.
3.90.740.10. 1 hit.
HAMAPiMF_00049_B. Leu_tRNA_synth_B.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase.
IPR025709. Leu_tRNA-synth_edit.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERiPTHR11946:SF7. PTHR11946:SF7. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 3 hits.
PF13603. tRNA-synt_1_2. 1 hit.
[Graphical view]
PRINTSiPR00985. TRNASYNTHLEU.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00396. leuS_bact. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Novel metabolic attributes of the genus Cyanothece, comprising a group of unicellular nitrogen-fixing Cyanobacteria."
    Bandyopadhyay A., Elvitigala T., Welsh E., Stockel J., Liberton M., Min H., Sherman L.A., Pakrasi H.B.
    MBio 2:E214-E214(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: PCC 7425 / ATCC 29141.

Entry informationi

Entry nameiSYL_CYAP4
AccessioniPrimary (citable) accession number: B8HM05
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: March 3, 2009
Last modified: April 1, 2015
This is version 46 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.