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Protein

Photosystem I P700 chlorophyll a apoprotein A2

Gene

psaB

Organism
Cyanothece sp. (strain PCC 7425 / ATCC 29141)
Status
Unreviewed-Annotation score: Annotation score: 4 out of 5-Protein inferred from homologyi

Functioni

PsaA and PsaB bind P700, the primary electron donor of photosystem I (PSI), as well as the electron acceptors A0, A1 and FX. PSI is a plastocyanin/cytochrome c6-ferredoxin oxidoreductase, converting photonic excitation into a charge separation, which transfers an electron from the donor P700 chlorophyll pair to the spectroscopically characterized acceptors A0, A1, FX, FA and FB in turn. Oxidized P700 is reduced on the lumenal side of the thylakoid membrane by plastocyanin or cytochrome c6.UniRule annotation
PsaA and psaB bind P700, the primary electron donor of photosystem I (PSI), as well as the electron acceptors A0, A1 and FX.UniRule annotation

Catalytic activityi

Reduced plastocyanin + oxidized ferredoxin + light = oxidized plastocyanin + reduced ferredoxin.UniRule annotation

Cofactori

Note: PSI electron transfer chain: 5 chlorophyll a, 1 chlorophyll a', 2 phylloquinones and 3 4Fe-4S clusters. PSI core antenna: 90 chlorophyll a, 22 carotenoids, 3 phospholipids and 1 galactolipid. P700 is a chlorophyll a/chlorophyll a' dimer, A0 is one or more chlorophyll a, A1 is one or both phylloquinones and FX is a shared 4Fe-4S iron-sulfur center.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi567 – 5671Iron-sulfur (4Fe-4S); shared with dimeric partnerUniRule annotation
Metal bindingi576 – 5761Iron-sulfur (4Fe-4S); shared with dimeric partnerUniRule annotation
Metal bindingi662 – 6621Magnesium (chlorophyll-a B1 axial ligand; P700 special pair)UniRule annotation
Metal bindingi670 – 6701Magnesium (chlorophyll-a B3 axial ligand)UniRule annotation
Binding sitei678 – 6781Chlorophyll-a B3UniRule annotation
Binding sitei679 – 6791Phylloquinone BUniRule annotation

GO - Molecular functioni

  1. 4 iron, 4 sulfur cluster binding Source: UniProtKB-KW
  2. chlorophyll binding Source: UniProtKB-KW
  3. electron carrier activity Source: UniProtKB-HAMAP
  4. magnesium ion binding Source: UniProtKB-HAMAP
  5. oxidoreductase activity Source: UniProtKB-KW

GO - Biological processi

  1. photosynthesis Source: UniProtKB-HAMAP
  2. protein-chromophore linkage Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

OxidoreductaseUniRule annotation

Keywords - Biological processi

Electron transportUniRule annotation, Photosynthesis, Transport

Keywords - Ligandi

4Fe-4SUniRule annotation, ChlorophyllUniRule annotation, Chromophore, Iron, Iron-sulfur, MagnesiumUniRule annotation, Metal-binding

Enzyme and pathway databases

BioCyciCSP395961:GJDE-4516-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Photosystem I P700 chlorophyll a apoprotein A2UniRule annotation (EC:1.97.1.12UniRule annotation)
Alternative name(s):
PsaBUniRule annotation
Gene namesi
Name:psaBUniRule annotation
Ordered Locus Names:Cyan7425_4670Imported
OrganismiCyanothece sp. (strain PCC 7425 / ATCC 29141)Imported
Taxonomic identifieri395961 [NCBI]
Taxonomic lineageiBacteriaCyanobacteriaOscillatoriophycideaeChroococcalesCyanothece
ProteomesiUP000002511: Chromosome

Subcellular locationi

Cellular thylakoid membrane UniRule annotation; Multi-pass membrane protein UniRule annotation
Membrane UniRule annotation; Multi-pass membrane protein UniRule annotation

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei39 – 7032Helical; Name=IUniRule annotationAdd
BLAST
Transmembranei132 – 15625Helical; Name=IIUniRule annotationAdd
BLAST
Transmembranei173 – 19523Helical; Name=IIIUniRule annotationAdd
BLAST
Transmembranei270 – 28718Helical; Name=IVUniRule annotationAdd
BLAST
Transmembranei336 – 35924Helical; Name=VUniRule annotationAdd
BLAST
Transmembranei370 – 40132Helical; Name=VIUniRule annotationAdd
BLAST
Transmembranei421 – 45030Helical; Name=VIIUniRule annotationAdd
BLAST
Transmembranei522 – 54625Helical; Name=VIIIUniRule annotationAdd
BLAST
Transmembranei580 – 61132Helical; Name=IXUniRule annotationAdd
BLAST
Transmembranei652 – 67322Helical; Name=XUniRule annotationAdd
BLAST
Transmembranei710 – 73829Helical; Name=XIUniRule annotationAdd
BLAST

GO - Cellular componenti

  1. integral component of membrane Source: UniProtKB-KW
  2. photosystem I Source: UniProtKB-KW
  3. thylakoid membrane Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Membrane, Photosystem IUniRule annotation, ThylakoidUniRule annotation

Interactioni

Subunit structurei

The PsaA/B heterodimer binds the P700 chlorophyll special pair and subsequent electron acceptors. PSI consists of a core antenna complex that captures photons, and an electron transfer chain that converts photonic excitation into a charge separation. The cyanobacterial PSI reaction center is composed of one copy each of PsaA,B,C,D,E,F,I,J,K,L,M and X, and forms trimeric complexes.UniRule annotation

Protein-protein interaction databases

STRINGi395961.Cyan7425_4670.

Family & Domainsi

Sequence similaritiesi

Belongs to the PsaA/PsaB family.UniRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helixUniRule annotation

Phylogenomic databases

eggNOGiNOG04867.
HOGENOMiHOG000276868.
KOiK02690.
OMAiAQFNESS.
OrthoDBiEOG60W7PS.

Family and domain databases

Gene3Di1.20.1130.10. 1 hit.
HAMAPiMF_00482. PSI_PsaB.
InterProiIPR001280. PSI_PsaA/B.
IPR020586. PSI_PsaA/B_CS.
IPR006244. PSI_PsaB.
[Graphical view]
PfamiPF00223. PsaA_PsaB. 1 hit.
[Graphical view]
PIRSFiPIRSF002905. PSI_A. 1 hit.
PRINTSiPR00257. PHOTSYSPSAAB.
SUPFAMiSSF81558. SSF81558. 1 hit.
TIGRFAMsiTIGR01336. psaB. 1 hit.
PROSITEiPS00419. PHOTOSYSTEM_I_PSAAB. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

B8HLB1-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MATRFPKFSQ DLAQDPTTRR IWYAIATAHD FESHDGMTEE NLYQKIFASH
60 70 80 90 100
FGHLAIIFLW TSGSLFHVAW QGNFEQWIKD PLNIRPIAHA IWDPQFGKPA
110 120 130 140 150
VDAFTQAGAS SPVDIAYSGV YHWWYTIGMR TNSDLYMGAI TLLLFAAVFL
160 170 180 190 200
FAGWLHLQPK FRPSLAWFKN AESRLNHHLA GLFGVSSLAW AGHLIHVAIP
210 220 230 240 250
EARGQHVGWD NFLSTMPHPA GLAPFFTGNW GVYAQNPDTA GHVFGTSEGA
260 270 280 290 300
GTAILTFLGG FHPQTESLWL TDIAHHHLAI AVIFIIAGHM YRTNFGIGHS
310 320 330 340 350
MKEIMNAHNP PQGTPFGGML GAGHQGMYDT YNNSLHFQLG WHLACLGVVT
360 370 380 390 400
SLVAQHMYSL PPYAFIAQDH TTMAALYTHH QYIAGFLMVG AFAHGAIFLV
410 420 430 440 450
RDYDPAANKN NVLARVLEHK EAIISHLSWV SLFLGFHTLG LYVHNDVVVA
460 470 480 490 500
FGTPEKQILI EPVFAQFIQA AHGKVLYGFD TLLSNPDSIA TTAWPNYGNV
510 520 530 540 550
WLPGWLDAIN SGTNSLFLTI GPGDFLVHHA IALGLHTTTL ILVKGALDAR
560 570 580 590 600
GSKLMPDKKD FGYAFPCDGP GRGGTCDISG WDAFYLSMFW MLNTIGWVTF
610 620 630 640 650
YWHWKHLGIW EGNVAQFNES STYLMGWLRD YLWLYSGPLI NGYNPFGMNN
660 670 680 690 700
LAVWSWMFLF GHLVWATGFM FLISWRGYWQ ELIETLVWAH ERTPLANLVR
710 720 730 740
WKDKPVALSI VQARLVGLAH FTVGYIVTYA AFLIASTAAK YG
Length:742
Mass (Da):82,907
Last modified:March 3, 2009 - v1
Checksum:i96B36B749D0A0B07
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001344 Genomic DNA. Translation: ACL46976.1.
RefSeqiWP_012630018.1. NC_011884.1.
YP_002485337.1. NC_011884.1.

Genome annotation databases

EnsemblBacteriaiACL46976; ACL46976; Cyan7425_4670.
GeneIDi7290623.
KEGGicyn:Cyan7425_4670.
PATRICi21570339. VBICyaSp30657_4659.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001344 Genomic DNA. Translation: ACL46976.1.
RefSeqiWP_012630018.1. NC_011884.1.
YP_002485337.1. NC_011884.1.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi395961.Cyan7425_4670.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiACL46976; ACL46976; Cyan7425_4670.
GeneIDi7290623.
KEGGicyn:Cyan7425_4670.
PATRICi21570339. VBICyaSp30657_4659.

Phylogenomic databases

eggNOGiNOG04867.
HOGENOMiHOG000276868.
KOiK02690.
OMAiAQFNESS.
OrthoDBiEOG60W7PS.

Enzyme and pathway databases

BioCyciCSP395961:GJDE-4516-MONOMER.

Family and domain databases

Gene3Di1.20.1130.10. 1 hit.
HAMAPiMF_00482. PSI_PsaB.
InterProiIPR001280. PSI_PsaA/B.
IPR020586. PSI_PsaA/B_CS.
IPR006244. PSI_PsaB.
[Graphical view]
PfamiPF00223. PsaA_PsaB. 1 hit.
[Graphical view]
PIRSFiPIRSF002905. PSI_A. 1 hit.
PRINTSiPR00257. PHOTSYSPSAAB.
SUPFAMiSSF81558. SSF81558. 1 hit.
TIGRFAMsiTIGR01336. psaB. 1 hit.
PROSITEiPS00419. PHOTOSYSTEM_I_PSAAB. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Novel metabolic attributes of the genus Cyanothece, comprising a group of unicellular nitrogen-fixing Cyanobacteria."
    Bandyopadhyay A., Elvitigala T., Welsh E., Stockel J., Liberton M., Min H., Sherman L.A., Pakrasi H.B.
    MBio 2:E214-E214(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: PCC 7425 / ATCC 29141Imported.

Entry informationi

Entry nameiB8HLB1_CYAP4
AccessioniPrimary (citable) accession number: B8HLB1
Entry historyi
Integrated into UniProtKB/TrEMBL: March 3, 2009
Last sequence update: March 3, 2009
Last modified: January 7, 2015
This is version 47 of the entry and version 1 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteomeImported

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.