B8HH36 (DXS_ARTCA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
1-deoxy-D-xylulose-5-phosphate synthase
EC=2.2.1.7

Alternative name(s):
1-deoxyxylulose-5-phosphate synthase
Short name=DXP synthase
Short name=DXPS

Gene names

Name:	dxs
Ordered Locus Names:	Achl_1638

Organism

Arthrobacter chlorophenolicus (strain A6 / ATCC 700700 / DSM 12829 / JCM 12360) [Complete proteome] [HAMAP]

Taxonomic identifier

452863 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Micrococcineae › Micrococcaceae › Arthrobacter

Protein attributes

Sequence length	657 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the acyloin condensation reaction between C atoms 2 and 3 of pyruvate and glyceraldehyde 3-phosphate to yield 1-deoxy-D-xylulose-5-phosphate (DXP) By similarity. HAMAP MF_00315
Catalytic activity	Pyruvate + D-glyceraldehyde 3-phosphate = 1-deoxy-D-xylulose 5-phosphate + CO2. HAMAP MF_00315
Cofactor	Binds 1 thiamine pyrophosphate per subunit By similarity.
Pathway	Metabolic intermediate biosynthesis; 1-deoxy-D-xylulose 5-phosphate biosynthesis; 1-deoxy-D-xylulose 5-phosphate from D-glyceraldehyde 3-phosphate and pyruvate: step 1/1. HAMAP MF_00315
Subunit structure	Homodimer By similarity. HAMAP MF_00315
Sequence similarities	Belongs to the transketolase family. DXPS subfamily.

Ontologies

Keywords
Biological process	Isoprene biosynthesis Thiamine biosynthesis
Ligand	Thiamine pyrophosphate
Molecular function	Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	terpenoid biosynthetic process Inferred from electronic annotation. Source: InterPro thiamine biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	1-deoxy-D-xylulose-5-phosphate synthase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 657	657	1-deoxy-D-xylulose-5-phosphate synthase HAMAP MF_00315		PRO_1000132919

Sequences

	Sequence		Length	Mass (Da)
	B8HH36 [UniParc]. Last modified March 3, 2009. Version 1. Checksum: EE3BBF08E19F395D	FASTA	657	70,312
	10 20 30 40 50 60 MGILDTIRNP QDLNDLSEEQ LEQLASEIRE FLITNVSQTG GHLGPNLGVV ELTLAVHRIF 70 80 90 100 110 120 DSPRDSIVFD TGHQSYVHKL LTGRQDFSTL RQQGGLSGYP SRAESEHDIV ESSHASSSLS 130 140 150 160 170 180 WADGISRARQ LTGEGDRHVV AVVGDGALTG GMAWEAINNI AADKKRRVVI VVNDNGRSYA 190 200 210 220 230 240 PTVGGFADYL ASLRPTIDSF RAAPAYEVAL DWWKKKLQNG GPAGQFTYKS LHAMKKGVKD 250 260 270 280 290 300 WWAPQGMFED LGMKYIGPVD GHNLQALEHA LATARNYHGP VIVHAMTEKG HGYAPARAHE 310 320 330 340 350 360 ADQFHAVGII DPETGEPTGT AGAKSWTSVF ADEIAAIADE RKDVVGITGA MLIPVGLHKF 370 380 390 400 410 420 AAKHPDRVID VGIAEQHALT SAAGMAFGGL HPVVAVYATF LNRAFDQLLM DVALHKAGVT 430 440 450 460 470 480 IVLDRAGVTG PDGASHHGMW DMSMVQIVPG LHLAAPRDAS RLREELREAV AINDAPTVVR 490 500 510 520 530 540 FSKGSVGAEI EALERLSDGV DVLARRPAGS TANDVLIVSV GAMSELALDV ANRLGAQGIS 550 560 570 580 590 600 TTVVDPRWVL PVRRSIVALA SHHRLVICIE DGVRAGGVGS RIRQEMRAAG VDTALNEVGL 610 620 630 640 650 PVEFLDHGTR SEVLERVGLT AQQITHDVVA QVLGTKVPFA RPLPGQQHPT TGSLPIL

References

[1]

"Complete sequence of chromosome of Arthrobacter chlorophenolicus A6." US DOE Joint Genome Institute Lucas S., Copeland A., Lapidus A., Glavina del Rio T., Tice H., Bruce D., Goodwin L., Pitluck S., Goltsman E., Clum A., Larimer F., Land M., Hauser L., Kyrpides N., Mikhailova N., Jansson J., Richardson P. Submitted (JAN-2009) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: A6 / ATCC 700700 / DSM 12829 / JCM 12360.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP001341 Genomic DNA. Translation: ACL39625.1.
RefSeq	YP_002487714.1. NC_011886.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	B8HH36.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	7293091.
GenomeReviews	Gene locus Achl_1638 in contig CP001341_GR.
KEGG	ach:Achl_1638.
PATRIC	20989845. VBIArtChl38304_2313.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG571647.
OMA	DPILYHG.
ProtClustDB	PRK05444.
Family and domain databases
HAMAP	MF_00315. DXP_synth. [Tree]
InterPro	IPR005477. Dxylulose-5-P_synthase. IPR009014. Transketo_C/Pyr-ferredox_oxred. IPR015941. Transketolase-like_C. IPR005475. Transketolase-like_Pyr-bd. IPR020826. Transketolase_BS. IPR005476. Transketolase_C. IPR005474. Transketolase_N. [Graphical view]
Gene3D	G3DSA:3.40.50.920. Transketo_C_like. 1 hit.
KO	K01662.
Pfam	PF02779. Transket_pyr. 1 hit. PF02780. Transketolase_C. 1 hit. [Graphical view]
SMART	SM00861. Transket_pyr. 1 hit. [Graphical view]
SUPFAM	SSF52922. Transketo_C_like. 1 hit.
TIGRFAMs	TIGR00204. Dxs. 1 hit.
PROSITE	PS00801. TRANSKETOLASE_1. 1 hit. PS00802. TRANSKETOLASE_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DXS_ARTCA

Accession

Primary (citable) accession number: B8HH36

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 28, 2009
Last sequence update:	March 3, 2009
Last modified:	January 25, 2012
This is version 20 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program