B8HGW8 (B8HGW8_ARTCA) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names

Recommended name:
UDP-N-acetylmuramoylalanine--D-glutamate ligase HAMAP MF_00639
EC=6.3.2.9 HAMAP MF_00639

Alternative name(s):
D-glutamic acid-adding enzyme HAMAP MF_00639
UDP-N-acetylmuramoyl-L-alanyl-D-glutamate synthetase HAMAP MF_00639

Gene names

Name:	murD HAMAP MF_00639
Ordered Locus Names:	Achl_1569

Organism

Arthrobacter chlorophenolicus (strain A6 / ATCC 700700 / DSM 12829 / JCM 12360) [Complete proteome] [HAMAP]

Taxonomic identifier

452863 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Micrococcineae › Micrococcaceae › Arthrobacter

Protein attributes

Sequence length	525 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Cell wall formation. Catalyzes the addition of glutamate to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanine (UMA) By similarity. HAMAP MF_00639 SAAS SAAS005762
Catalytic activity	ATP + UDP-N-acetylmuramoyl-L-alanine + glutamate = ADP + phosphate + UDP-N-acetylmuramoyl-L-alanyl-D-glutamate. HAMAP MF_00639 SAAS SAAS005762
Pathway	Cell wall biogenesis; peptidoglycan biosynthesis. HAMAP MF_00639 SAAS SAAS005762
Subcellular location	Cytoplasm By similarity HAMAP MF_00639 SAAS SAAS005762.
Sequence similarities	Belongs to the MurCDEF family. HAMAP MF_00639

Ontologies

Keywords
Biological process	Cell cycle Cell division HAMAP MF_00639 SAAS SAAS005762 Cell shape Cell wall biogenesis/degradation HAMAP MF_00639 SAAS SAAS005762 Peptidoglycan synthesis HAMAP MF_00639 SAAS SAAS005762
Cellular component	Cytoplasm HAMAP MF_00639 SAAS SAAS005762
Ligand	ATP-binding HAMAP MF_00639 SAAS SAAS005762 Nucleotide-binding
Molecular function	Ligase HAMAP MF_00639 SAAS SAAS005762 EMBL ACL39557.1
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cell cycle Inferred from electronic annotation. Source: UniProtKB-KW cell division Inferred from electronic annotation. Source: UniProtKB-KW cellular cell wall organization Inferred from electronic annotation. Source: UniProtKB-KW peptidoglycan biosynthetic process Inferred from electronic annotation. Source: HAMAP regulation of cell shape Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP NADP binding Inferred from electronic annotation. Source: InterPro UDP-N-acetylmuramoylalanine-D-glutamate ligase activity Inferred from electronic annotation. Source: HAMAP flavin adenine dinucleotide binding Inferred from electronic annotation. Source: InterPro flavin-containing monooxygenase activity Inferred from electronic annotation. Source: InterPro tetrahydrofolylpolyglutamate synthase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Regions
	Nucleotide binding	146 – 152	7	ATP By similarity HAMAP MF_00639

Sequences

	Sequence		Length	Mass (Da)
	B8HGW8 [UniParc]. Last modified March 3, 2009. Version 1. Checksum: C552DC828D10FBB7	FASTA	525	55,326
	10 20 30 40 50 60 MGCAAVTVSP RLENLVTWDS DWSGLRVAVT GIGVSGFAAA DTLIELGARV VVVDAATSDT 70 80 90 100 110 120 AKASADTLKI VGAADVLLGE EAVTRIPRID GELPELIVTS PGWRPDQALL AAAARAHIPV 130 140 150 160 170 180 WGDVELAWRV RRREGRKTAD WLAITGTNGK TTTVGLTESM LRAAGLKAIA VGNVGKPILD 190 200 210 220 230 240 AIREPVEYDV FAVELSSFQL HWAESLSPLA SVCLNVAEDH VDWHGSYDSY LADKAKVYER 250 260 270 280 290 300 TQKACLYNAE QFETERMVEN ADVVEGCRAI GFTTVTPAIS MLGVVEGLLV DRAFIEERRD 310 320 330 340 350 360 SAAELAAMAD LGPIAPRHMV ANALAAAGLV RAYGVDAAAV RQGIRDYVPG DHRIQPVARH 370 380 390 400 410 420 EGVLWVNDSK ATNPHAAAAS LAAFTNVVWI AGGLSKGVTY DDLVREQAHR LKAVVLIGTD 430 440 450 460 470 480 STELAGALQR HAPDVPVIAA APGETEEVQT AAAEGATGTR FPLSGEQVMS QAVATAAQLA 490 500 510 520 GSGDTVLMAP AAASMDQFSS YAHRGNTFIE AVRGLVEGQA KTGEE

References

[1]

"Complete sequence of chromosome of Arthrobacter chlorophenolicus A6." Lucas S., Copeland A., Lapidus A., Glavina del Rio T., Tice H., Bruce D., Goodwin L., Pitluck S., Goltsman E., Clum A., Larimer F., Land M., Hauser L., Kyrpides N., Mikhailova N., Jansson J., Richardson P. Submitted (JAN-2009) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: A6 / ATCC 700700 / DSM 12829 / JCM 12360.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP001341 Genomic DNA. Translation: ACL39557.1.
RefSeq	YP_002487646.1. NC_011886.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	B8HGW8.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	7293022.
GenomeReviews	Gene locus Achl_1569 in contig CP001341_GR.
KEGG	ach:Achl_1569.
PATRIC	20989699. VBIArtChl38304_2240.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG750024.
OMA	ACASWDM.
ProtClustDB	CLSK959755.
Family and domain databases
HAMAP	MF_00639. MurD. [Tree]
InterPro	IPR000960. Flavin_mOase. IPR018109. Folylpolyglutamate_synth_CS. IPR004101. Mur_ligase_C. IPR013221. Mur_ligase_cen. IPR016040. NAD(P)-bd_dom. IPR005762. UDP-N-AcMur-Glu_ligase. [Graphical view]
Gene3D	G3DSA:3.90.190.20. Mur_ligase_C. 1 hit. G3DSA:3.40.1190.10. Mur_ligase_cen. 1 hit. G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
KO	K01925.
PANTHER	PTHR23135:SF2. PTHR23135:SF2. 1 hit.
Pfam	PF02875. Mur_ligase_C. 1 hit. PF08245. Mur_ligase_M. 1 hit. [Graphical view]
PRINTS	PR00370. FMOXYGENASE.
SUPFAM	SSF53244. Mur_ligase_C. 1 hit. SSF53623. Mur_ligase_cen. 1 hit.
TIGRFAMs	TIGR01087. MurD. 1 hit.
PROSITE	PS01011. FOLYLPOLYGLU_SYNT_1. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

B8HGW8_ARTCA

Accession

Primary (citable) accession number: B8HGW8

Entry history

Integrated into UniProtKB/TrEMBL:	March 3, 2009
Last sequence update:	March 3, 2009
Last modified:	December 14, 2011
This is version 21 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)